BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0047.p (113 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311377|ref|YP_003775469.1| flagellar hook-basal body prote... 148 9e-38 >gi|300311377|ref|YP_003775469.1| flagellar hook-basal body protein [Herbaspirillum seropedicae SmR1] Length = 118 Score = 148 bits (374), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 81/113 (71%), Positives = 97/113 (85%) Query: 1 MIDTSRIEAMVSQLKAAAARAQGNVAPVESITGGEKPAQRVDFASVLKNSLDEVSASQEK 60 +IDTS+IEAMV+QLKAAAARAQG P + EK A RVDF+ VLK ++D+V+ SQ+K Sbjct: 6 VIDTSKIEAMVAQLKAAAARAQGVSEPAQIGLKVEKAAPRVDFSEVLKGAIDQVNDSQQK 65 Query: 61 SKKMSEAFSMGDDNINLSDVMIAMQKSSISFQTSLQVRNKLVSAYRDIMTMQV 113 + KMS+AF+MGDDN+NLSDVMIA QK+SISFQTS+QVRNKLVSAYRDIMTMQV Sbjct: 66 ASKMSQAFAMGDDNVNLSDVMIASQKASISFQTSIQVRNKLVSAYRDIMTMQV 118 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.121 0.300 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 250,480 Number of extensions: 7412 Number of successful extensions: 22 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 22 Number of HSP's successfully gapped: 1 Length of query: 113 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 40 Effective length of database: 1,274,215 Effective search space: 50968600 Effective search space used: 50968600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 124 (52.4 bits)