BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0045.p (95 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311379|ref|YP_003775471.1| flagellar biosynthetic protein ... 164 2e-42 gi|300311341|ref|YP_003775433.1| flagellar biosynthesis FlhB tra... 53 4e-09 >gi|300311379|ref|YP_003775471.1| flagellar biosynthetic protein [Herbaspirillum seropedicae SmR1] Length = 114 Score = 164 bits (414), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 80/93 (86%), Positives = 86/93 (92%) Query: 1 MNKPKLPFQNAVALAYNTNQAAPKVVAKGSGLVAEAIIARARESGVFVHESKELVSLLMQ 60 M KP +P QNAVALAYN +QAAP+VVAKG GLVAEAIIARARESGV+VHESKELVSLLMQ Sbjct: 1 MEKPNIPLQNAVALAYNASQAAPQVVAKGRGLVAEAIIARARESGVYVHESKELVSLLMQ 60 Query: 61 VDLDQQIPPALYRAVAELLAWLYRIENMQLDRS 93 V+LDQQIPPALYR VAELLAWLYRIENMQL+ S Sbjct: 61 VELDQQIPPALYRTVAELLAWLYRIENMQLNSS 93 >gi|300311341|ref|YP_003775433.1| flagellar biosynthesis FlhB transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 391 Score = 53.1 bits (126), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 35/83 (42%), Positives = 50/83 (60%), Gaps = 2/83 (2%) Query: 7 PFQNAVALAYNTNQ-AAPKVVAKGSGLVAEAIIARARESGVFVHESKELV-SLLMQVDLD 64 P AVAL Y+ + APKVVAKGS +A I ARE V + E+ L +L ++ Sbjct: 270 PTHYAVALKYSEGKMGAPKVVAKGSDEIAAKIRELAREHRVPLLEAPPLARALHTHTEIG 329 Query: 65 QQIPPALYRAVAELLAWLYRIEN 87 +IP ALY AVAE+LA+++++ Sbjct: 330 DEIPEALYTAVAEVLAYVFQLRT 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 267,235 Number of extensions: 8666 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 34 Number of HSP's successfully gapped: 2 Length of query: 95 Length of database: 1,619,870 Length adjustment: 64 Effective length of query: 31 Effective length of database: 1,316,830 Effective search space: 40821730 Effective search space used: 40821730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)