BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0018.p (451 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 826 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 107 3e-24 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 100 3e-22 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 92 8e-20 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 84 2e-17 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 826 bits (2134), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/408 (95%), Positives = 401/408 (98%) Query: 43 VRPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR 102 +RPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR Sbjct: 1 MRPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR 60 Query: 103 NMGNASGYTDSKGLFAPRKSVMHYTQQKNIQGVAIDDIYLGNGASELIVMSVNALLNTGD 162 NMGNASGYTDSKGLFAPRKSVMHYTQQK I+GV IDDIYLGNGASELIVMSVNALLNTGD Sbjct: 61 NMGNASGYTDSKGLFAPRKSVMHYTQQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGD 120 Query: 163 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNP 222 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADI+KKITPNTKAIVVINPNNP Sbjct: 121 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNP 180 Query: 223 TGALYSTEVLKELVEVARQHQLIIFADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK 282 TGALYS EVLKE++E+ARQHQLII ADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK Sbjct: 181 TGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK 240 Query: 283 NYRSCGYRAGWMVVSGEKKHAKDYIEGLNMLASMRLCANAPGQYAIQTALGGYQSINDLV 342 NYRSCGYRAGWMVVSGEKKHA+DYIEGLNMLASMRLCANAPGQ+AIQTALGGYQSINDLV Sbjct: 241 NYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLV 300 Query: 343 APTGRLAKQRDLAHKLLTDIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK 402 P GRL KQRDLAH+LLTDIPGV+CVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK Sbjct: 301 GPQGRLTKQRDLAHQLLTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK 360 Query: 403 VLIVQGTGFNCPTPDHFRVVFLPNTDDLTESMGRIAHFLEGYRRRHGT 450 VLIVQGTGFNCPTPDHFRVVFLPNTDDLTESMGRIAHFLEGYRRRHGT Sbjct: 361 VLIVQGTGFNCPTPDHFRVVFLPNTDDLTESMGRIAHFLEGYRRRHGT 408 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 107 bits (266), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 97/392 (24%), Positives = 179/392 (45%), Gaps = 21/392 (5%) Query: 48 KSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR--NMG 105 + ++L ++I + AR+ G II +++GN P IV+ ++ Sbjct: 43 RIERLPPYVFNITAELKMAARR---RGEDIIDMSMGNPDG---ATPPHIVEKLVEVAQRP 96 Query: 106 NASGYTDSKGLFAPRKSVMH-YTQQKNIQGVAIDDIYLGNGASELIVMSVNALLNTGDEV 164 + GY+ SKG+ R+++ H Y + + + + G+ E + + A L+ GD V Sbjct: 97 DTHGYSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTV 156 Query: 165 LVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTG 224 LVP+P YP+ ++G + ++ ++ P K +++ P+NPT Sbjct: 157 LVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTA 216 Query: 225 ALYSTEVLKELVEVARQHQLIIFADEIYDKVLYDGNTHTSLASL--ADDVLFITFNGLSK 282 E + +V++AR+HQ+++ D Y + +DG S+ + A +V + F LSK Sbjct: 217 QCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVA-VEFFTLSK 275 Query: 283 NYRSCGYRAGWMVVSGEKKHAKDYIEGLNMLASMRLCANAPGQYAIQTALGGYQSINDLV 342 +Y G+R G+MV + A I+ + S P Q A AL G QS + + Sbjct: 276 SYNMAGWRIGFMVGNARLVAALARIKSYHDYGSF-----TPVQVAAIAALEGDQSCVEEI 330 Query: 343 APTGRLAKQRDLAHKLLTDIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK 402 ++R++ K L + G PK+++Y++ R+ PE Y EFA LL + K Sbjct: 331 --RANYERRRNVLVKGLHEA-GWMVDVPKASMYIWARI-PEPYRQFGSLEFARILLEQAK 386 Query: 403 VLIVQGTGFNCPTPDHFRVVFLPNTDDLTESM 434 V + G GF ++ R + N + +++ Sbjct: 387 VCVSPGIGFGEYGDEYVRFALIENESRIRQAI 418 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 100/387 (25%), Positives = 178/387 (45%), Gaps = 23/387 (5%) Query: 65 EKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNAS-GYTDSKGLFAPRKSV 123 +KA +E++G +I + IG F AP +V+ R M YT + GL R ++ Sbjct: 23 KKAAVLEQQGRHLIHMGIGEP---DFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAI 79 Query: 124 M-HYTQQKNIQGVAIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSG 182 HY ++ +A + I + GAS ++++ AL+ EVL+P P YP V+ Sbjct: 80 SDHYRSVYGLE-IAPERIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFE 138 Query: 183 GTPVHYVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQH 242 G + +Q +R+ T+ +++ +P+NPTG + L+ +V RQ Sbjct: 139 GRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQR 198 Query: 243 QLIIFADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKH 302 DEIY + Y+G ++L SL DDV+ I N SK + G+R GW+V+ Sbjct: 199 GGFTIVDEIYQGLSYEGAPFSAL-SLGDDVVVI--NSFSKYFNMTGWRLGWLVLP----- 250 Query: 303 AKDYIEGLNMLA-SMRLCANAPGQYAIQTALGGYQSINDLVAPTGRLAKQRDLAHKLLTD 361 + + LA ++ +CA++ Q+A A Q++ ++RD L Sbjct: 251 -PALVPQVEKLAQNLLICASSIAQHA-AVACFTPQTLALYEERKAEFKRRRDYIVPALES 308 Query: 362 IPGVTCVKPKSALYMFPRLDPEIYPIKDD-QEFAYELLAEEKVLIVQGTGFNCPTPDHF- 419 + V P A Y++ + + DD + + ++L E V++V G F T + Sbjct: 309 LGFTVPVMPDGAFYVY----ADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARRYI 364 Query: 420 RVVFLPNTDDLTESMGRIAHFLEGYRR 446 R+ + + ++L E++ R+ F E R+ Sbjct: 365 RLSYATSMENLQEAVARLRVFFEKRRK 391 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 92.0 bits (227), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 59/197 (29%), Positives = 100/197 (50%), Gaps = 13/197 (6%) Query: 137 IDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDE--QQ 194 + +I + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV +V E Q Sbjct: 89 VSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPV-FVQMEVGAQ 147 Query: 195 GWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLIIFADEIYDK 254 G+ ++ +TP T+ I+V P+NPTG++ + L ++ R ++I +DE+Y+ Sbjct: 148 GYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEH 207 Query: 255 VLYDGNTHTSLA---SLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHAKDYIEGLN 311 ++YDG H SLA LA+ FI + K Y G++ G++ + N Sbjct: 208 MVYDGQPHESLARHPELAERS-FIN-SSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFN 265 Query: 312 MLASMRLCANAPGQYAI 328 + N P QY + Sbjct: 266 VFT-----VNTPVQYGL 277 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 86/387 (22%), Positives = 167/387 (43%), Gaps = 35/387 (9%) Query: 72 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQKN 131 + ++ I L IG P I Q +I N+ + Y + G A R ++ + Q++ Sbjct: 43 DPAYRPISLGIGEPK---HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRY 99 Query: 132 --IQGVAIDDIYLGNGASELIVMSVNALLNT---GDEVLVPSPDYPLWTAAVSLSGGTPV 186 + +I NG+ E + +++ G V+ P+P Y ++ + L+G P Sbjct: 100 DIPRPDPATEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPY 159 Query: 187 HYVCDEQQGWQPDIADIRKKITPNTKAIVVINPNNPTGALYSTEVLKELVEVARQHQLII 246 D ++ + PD + + + + + + V +P NPTGA+ + E KEL ++ ++ +I Sbjct: 160 FVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVI 219 Query: 247 FADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKK 301 +DE Y ++ ++ G + D I+F+ LSK G R+G+ V+G+ Sbjct: 220 ASDECYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGF--VAGDAA 277 Query: 302 HAKDYIEGLNMLASMRLCANAPGQYAIQTA-LGGYQSINDLVAPTGRLAKQRDLAHKLLT 360 K ++ + + A +P IQ A + +Q + + + LL Sbjct: 278 ILKKFL----LYRTYHGAAMSP---VIQAASVLAWQDETHVQGNIAKYVTKFSQVTPLLQ 330 Query: 361 DIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT-------GFNC 413 ++ + P +A Y++ ++D + D EFA L AE V ++ G+ G N Sbjct: 331 EV--LEVALPDAAFYLWAKVDKLVN--ISDTEFAQRLYAEYNVTVLPGSYLAREAHGVN- 385 Query: 414 PTPDHFRVVFLPNTDDLTESMGRIAHF 440 P R+ + ++ E+ RI F Sbjct: 386 PGAGRIRMALVAEVEECLEAAQRIVAF 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,337,779 Number of extensions: 54849 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 6 Length of query: 451 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 363 Effective length of database: 1,203,190 Effective search space: 436757970 Effective search space used: 436757970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)