BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0028.p (139 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312030|ref|YP_003776122.1| alkylphosphonate uptake protein... 167 3e-43 >gi|300312030|ref|YP_003776122.1| alkylphosphonate uptake protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 167 bits (423), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 91/112 (81%), Positives = 99/112 (88%), Gaps = 1/112 (0%) Query: 27 MSTLPPCPQCQSEFTYEDGALYICPECAHEWPTQPAEAVEESGKVWRDAAGNILQDGDTV 86 MSTLP CPQCQSE+TYEDG Y+CPECAHEW + A E +VWRDA+GN+LQDGDTV Sbjct: 1 MSTLPACPQCQSEYTYEDGGQYVCPECAHEW-SAQAAPAAEQARVWRDASGNVLQDGDTV 59 Query: 87 TVIKDLKLKGGGGVVKIGTKVKNIRLVDGDHDIDCKIDGFGQMGLKTEFVKK 138 TVIKDLKLKGGGGVVK+GTKVKNIRLVDGDHDIDCKIDGFG MGLKTEFV+K Sbjct: 60 TVIKDLKLKGGGGVVKMGTKVKNIRLVDGDHDIDCKIDGFGSMGLKTEFVRK 111 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 355,262 Number of extensions: 9851 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 1 Length of query: 139 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 63 Effective length of database: 1,260,010 Effective search space: 79380630 Effective search space used: 79380630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)