BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0025.p (434 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 655 0.0 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 264 1e-71 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 67 3e-12 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 655 bits (1690), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/431 (74%), Positives = 368/431 (85%), Gaps = 2/431 (0%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 +HLAAFLIAGNAAHSQ LWRHP+S GGFL LDYY IA+TLERGKFDLLFFADRLAVST Sbjct: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTSRIGLGATRSTTYSQPYSLAREFSTLD 122 R+G SH +G+ GDQDATR+DPLP+LGA+AA TS IGLGATRSTTYSQPYSLAREF+TLD Sbjct: 61 RYGDSHLHGLYIGDQDATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLD 120 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 HLS GRAAWNVVTSVNQGEADNFGL+ETL HD RYDRADEFLEVTHKLW+SW AL L+ Sbjct: 121 HLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQ 180 Query: 183 AGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 RYAD D V AIDH+G HFSVRGPLNIPASPQG PVIIQAGSS RGQDFAARWAEVV Sbjct: 181 --QDRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVV 238 Query: 243 FGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDKRARHNAMVD 302 F IQPDL R+Q FY+DLK+R ++F R+P DI++L+A+MPFVG+S+ A + + + NA+ D Sbjct: 239 FNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALAD 298 Query: 303 PIVGLSTLSSHMNVDFSAHALDAPIDDIQVAGMQGLFGLVKELSSEGRLTLADIGRLYAG 362 P+VGLSTLSSHMNVDFS ALDAP+ D+QV+G+QGLF L++ +S+E +LTLAD GRLYA Sbjct: 299 PLVGLSTLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLRAISAERQLTLADAGRLYAQ 358 Query: 363 GVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRGQFRL 422 GVL PQ+AGTAAD+ADW++ I+ GADGFVI+P+HLP GFDDFVD V+PELQRRG FR Sbjct: 359 GVLTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQ 418 Query: 423 DYEGDTLRSYL 433 DY G LR +L Sbjct: 419 DYAGRQLRQHL 429 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 264 bits (674), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 167/444 (37%), Positives = 228/444 (51%), Gaps = 28/444 (6%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 I L AF + + + LW HPR + + Y+ +A+ LERGKFD LF AD + V Sbjct: 6 IRLNAFNMNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGV-- 63 Query: 63 RFGGSHRYGVAHGDQDATRM--------DPLPVLGAIAAVTSRIGLGATRSTTYSQPYSL 114 Y V G+ D T DPL ++ A+AAVT +G G T + TY P+ Sbjct: 64 -------YDVYQGNTDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLF 116 Query: 115 AREFSTLDHLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWN-S 173 AR STLDHLS GR WN+VT A GL E L HD+RYDRADEF+EV +KLW S Sbjct: 117 ARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGS 176 Query: 174 WDAQALLLEAGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQD 233 W+ A+L + YADP VH I H G ++ V G S Q PV+ QAGSS RGQ Sbjct: 177 WEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQA 236 Query: 234 FAARWAEVVFGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDK 293 F AR AE VF ++ Q ++ + R P IK+ L G+++ A+++ Sbjct: 237 FGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQER 296 Query: 294 RARHNAMVDPIVGLSTLSSHMNVDFSAHALDAPIDDIQVAGMQGLFGLVK--ELSSEGRL 351 + P GL+ S+ +DFS + LD PI + ++ L +V EL+ R Sbjct: 297 YREYARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRK 356 Query: 352 TLA--DIGRLYAGGVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDL 409 L ++G YA + G +AD +E VA G DG+ +T PE F+DFV+L Sbjct: 357 LLQQLELGGRYAS------IVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVEL 410 Query: 410 VVPELQRRGQFRLDYEGDTLRSYL 433 V+P LQ RG ++ +Y TLR L Sbjct: 411 VIPVLQERGSYKREYAQGTLREKL 434 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 67.0 bits (162), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 104/394 (26%), Positives = 152/394 (38%), Gaps = 80/394 (20%) Query: 33 TLDYYKRIAQTLERGKFDLLFFADRLAVSTRFGGSHRYGVAHGDQDATRMDPLPVLGAIA 92 TL+Y ++IA T E +D L A+ +DP V ++ Sbjct: 28 TLEYMRQIAVTSETLGYDGLLIP---------------------TGASCLDPWVVAASLV 66 Query: 93 AVTSRIGLGATRSTTYSQPYSLAREFSTLDHLSAGRAAWNVVTSVNQGEADNFGLRETLG 152 VT RI L T+ P AR+ +TLD GR NVV + E + G+ L Sbjct: 67 PVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV--FLK 124 Query: 153 HDERYDRADEFLEVTHKLWNSWDAQALLLEAGSGRYADPDHVHAIDHRGTHFSVR-GPLN 211 HDERY+ ADE+L + KL +++ G HF +R G L Sbjct: 125 HDERYEAADEYLTIWRKLLQG---------------------ETVNYEGKHFRIRNGQLF 163 Query: 212 IPASPQGGPVIIQAGSSHRGQDFAARW--AEVVFGIQPDLARMQRFYQDLKSRTEKFCRK 269 PA Q P + GSS D AA+ A + +G P R Q + E+ R+ Sbjct: 164 HPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVR-----QKIDDVRERAARQ 218 Query: 270 PADIKVLTALMPFVGKSKAAAEDKRARHNAMVDPIVGLSTLSSHMNVDFSAHALDAPIDD 329 ++ L V ++ A A R ++ + + N A +D Sbjct: 219 GRTVRFGVRLHAIVRETTAEAWTAAER---LISKLTDEDIARAQQNY--------ARMDS 267 Query: 330 IQVAGMQGLFGLVKELSSEGRLTLADIG-RLYAGGVLVPQVAGT-----AADIADWMEHI 383 + M L G GR +I L+AG LV AGT A +A+ ++ Sbjct: 268 VGQQRMAALHG--------GRRDKLEIAPNLWAGVGLVRGGAGTSLVGDATTVAERLQEY 319 Query: 384 VAQQGADGFVITP-AHLPEGFDDFVDLVVPELQR 416 A G D FV++ HL E F +LV P L + Sbjct: 320 -ADLGVDTFVLSGYPHLEEAI-RFAELVFPLLGK 351 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,516,428 Number of extensions: 69581 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 3 Length of query: 434 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 346 Effective length of database: 1,203,190 Effective search space: 416303740 Effective search space used: 416303740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)