BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0021.p (87 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309702|ref|YP_003773794.1| translation initiation factor I... 131 9e-33 gi|300309477|ref|YP_003773569.1| translation initiation factor I... 94 2e-21 >gi|300309702|ref|YP_003773794.1| translation initiation factor IF-1 protein [Herbaspirillum seropedicae SmR1] Length = 87 Score = 131 bits (330), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 62/79 (78%), Positives = 70/79 (88%) Query: 1 MAKEELLEMNGLVEEVLPDSRFRVNLENGHRLIAYTSGKMRQNHIRILAGDKVTLELSPY 60 MAKEEL+EM G V EVLPDSRFRV L+NGH LIAYT+G+M++N I+ILAGDKVTLELSPY Sbjct: 1 MAKEELIEMQGQVSEVLPDSRFRVALDNGHSLIAYTAGRMKKNFIKILAGDKVTLELSPY 60 Query: 61 DLSKGRITFRHLEKRGPVG 79 DL+KGRI FRHLE RGP G Sbjct: 61 DLNKGRIIFRHLENRGPTG 79 >gi|300309477|ref|YP_003773569.1| translation initiation factor IF-1 [Herbaspirillum seropedicae SmR1] Length = 72 Score = 94.4 bits (233), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 42/70 (60%), Positives = 57/70 (81%) Query: 1 MAKEELLEMNGLVEEVLPDSRFRVNLENGHRLIAYTSGKMRQNHIRILAGDKVTLELSPY 60 MAK+++++M G + E LP++ FRV LENGH ++ + SGKMR N+IRIL GDKVT+EL+PY Sbjct: 1 MAKDDVIQMQGEILENLPNATFRVKLENGHVVLGHISGKMRMNYIRILPGDKVTVELTPY 60 Query: 61 DLSKGRITFR 70 DLS+ RI FR Sbjct: 61 DLSRARIVFR 70 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 220,090 Number of extensions: 7136 Number of successful extensions: 20 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 20 Number of HSP's successfully gapped: 2 Length of query: 87 Length of database: 1,619,870 Length adjustment: 57 Effective length of query: 30 Effective length of database: 1,349,975 Effective search space: 40499250 Effective search space used: 40499250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)