BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0015.p (422 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313995|ref|YP_003778087.1| urea ABC transporter periplasmi... 822 0.0 gi|300311947|ref|YP_003776039.1| branched-chain amino acid ABC t... 272 5e-74 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 62 6e-11 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 58 1e-09 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 58 1e-09 >gi|300313995|ref|YP_003778087.1| urea ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 822 bits (2122), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/421 (95%), Positives = 408/421 (96%), Gaps = 5/421 (1%) Query: 1 MSRRTILKATALGAFALAASSWLPAAFAADTIKVGILHSLSGTMAISETSLKDVALMTID 60 MSRRT+LKATALGAFALAASSWLP+AFAADTIKVGILHSLSGTMAISETSLKDVALMTID Sbjct: 1 MSRRTVLKATALGAFALAASSWLPSAFAADTIKVGILHSLSGTMAISETSLKDVALMTID 60 Query: 61 EINANGGVNGKKLEPVIVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFK 120 EINA GGV GKKLEPV+VDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFK Sbjct: 61 EINAAGGVMGKKLEPVVVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFK 120 Query: 121 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPATEYLMSKEGGGAKRFVLLGTDYVYP 180 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPA EYLMSKEGGGAKRFVLLGTDYVYP Sbjct: 121 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFVLLGTDYVYP 180 Query: 181 RTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFAAGGKTAVISTINGDSN 240 RTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKF+AGGKTAVISTINGDSN Sbjct: 181 RTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFSAGGKTAVISTINGDSN 240 Query: 241 VPFYKELGNAGLKATDVPVVAFSVGEEELRGVDAXGVDAKPLVGHLAAWNYFESVKNPVN 300 VPFYKELGNAGLKATDVPVVAFSVGEEELR GVD KPLVGHLAAWNYFESVKNPVN Sbjct: 241 VPFYKELGNAGLKATDVPVVAFSVGEEELR-----GVDTKPLVGHLAAWNYFESVKNPVN 295 Query: 301 TAFIKKWKAYAKAKGLPNADTVVTNDPMEATYVGIYMWKQAVEKAKTTDTDKVIAAMGGQ 360 TAFIKKWK YAKAK LPNADTVVTNDPMEATYVGI+MW QAVEKAK+TDTDKVIAAMGGQ Sbjct: 296 TAFIKKWKEYAKAKNLPNADTVVTNDPMEATYVGIHMWAQAVEKAKSTDTDKVIAAMGGQ 355 Query: 361 TFKAPSGYTLEMDKTNHHLHKPVMIGEIKADGQFNVVNKTKTTIRAQPWSPYIPGNESKQ 420 T+KAPSGYTLEMDKTNHHLHKPVMIGEIKADGQFNVV KTKTTIRAQPWSPYIPGNESKQ Sbjct: 356 TYKAPSGYTLEMDKTNHHLHKPVMIGEIKADGQFNVVYKTKTTIRAQPWSPYIPGNESKQ 415 Query: 421 K 421 K Sbjct: 416 K 416 >gi|300311947|ref|YP_003776039.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 272 bits (695), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 155/371 (41%), Positives = 222/371 (59%), Gaps = 21/371 (5%) Query: 32 IKVGILHSLSGTMAISETSLKDVALMTIDEINANGGVNGKKLEPVIVDPASNWPLFAEKA 91 + VG LHS +GTMAISET + ID+INA GG+ G+K++ + D AS+WP FAEKA Sbjct: 56 VTVGQLHSATGTMAISETGSIQAERLAIDQINAMGGILGRKIKIIQEDGASDWPTFAEKA 115 Query: 92 RQLISQDKVSVVFGCWTSVSRKSVLPVFKELNSLLFYPVQYEGEELEKNVFYTGAAPNQQ 151 ++L+ D+V+ VFGCWTS SRK+VLPVF++ N LL+YP YEG E KNVFYTG QQ Sbjct: 116 KKLLENDRVAAVFGCWTSASRKAVLPVFEKDNGLLYYPTFYEGLEQSKNVFYTGQEATQQ 175 Query: 152 AIPATEYLMSKEGGGAKRFVLLGTDYVYPRTTNKILRAFLHSKGVKDSDIDEVYTPFGHS 211 + ++ ++ AK F L+G+DY++PRT+NKI R + + +K S + E Y P G++ Sbjct: 176 VLAGLNWVAKEK--KAKTFFLVGSDYIWPRTSNKIARKHIENV-LKGSVVGEEYYPLGNT 232 Query: 212 DYQTIVANIKKFAAGGKTAVISTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRG 271 + +++ IK + + + G SNV FYK+L AG+ A ++ SV E+E+ G Sbjct: 233 QFGSLINKIK---LKKPDVIFADVVGGSNVAFYKQLKAAGVTAKTQNLLTISVTEDEMLG 289 Query: 272 VDAXGVDAKPLVGHLAAWNYFESVKNPVNTAFIKKWKAYAKAKGLPNADTVVTNDPMEAT 331 + V+ G A+ YF+S+ N N F+ ++KA A V D +A Sbjct: 290 IGGENVE-----GFFASMKYFQSLDNANNKKFVAEFKAKFGANS-------VIGDVTQAA 337 Query: 332 YVGIYMWKQAVEKAKTTDTDKVIAAMGGQTFK-APSGYTLEMDKTNHHLHKPVMIGEIKA 390 Y+G ++WK AVEKA + D DKV+AA G K AP GY + NHHL IGE + Sbjct: 338 YLGPWLWKAAVEKAGSFDVDKVVAASPGIELKTAPEGYVKVHE--NHHLWSKTRIGEAQK 395 Query: 391 DGQFNVVNKTK 401 DGQF VV +++ Sbjct: 396 DGQFKVVYESE 406 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 62.4 bits (150), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 101/406 (24%), Positives = 168/406 (41%), Gaps = 65/406 (16%) Query: 9 ATALGAFALAASSWLPAAFAADTIKVGILHSLSGTMAISETSLKDVALMTIDEINANGGV 68 A AL + AL + AAD IK+G+ +G A S++D + + EINA GG+ Sbjct: 10 AAALMSLALTSQ-------AADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGL 62 Query: 69 NGKKLEPVIVDPASNWPLFAEKARQLISQDKVSVVFG-CWTSVS-------RKSVLPVFK 120 G++++ + D + + A++LI+++KV G T V+ +++ +PV Sbjct: 63 LGRQIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVIN 122 Query: 121 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPATEYLMSKEGGGAKRFV---LLGTDY 177 + + Q+E E+ + VF T AA QA +++ E ++F +L Sbjct: 123 NVATGTVITKQFEKEK-DNYVFRTSAADKIQA-----KMIADEAVDKQKFTKVAILADST 176 Query: 178 VYPRTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQT----IVANIKKFAAGGKTAVIS 233 Y + + L L K V TP Y + A + K GG AV++ Sbjct: 177 NYGQLGREDLEKVLADKKV---------TPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLT 227 Query: 234 TINGDSNVPFYKELGNAGLKAT-DVPVVA---FSVGEEELRGVDAXGVDAKPLVGHLAAW 289 G P ++ N K VP++ S+G +D G + G Sbjct: 228 YGIG----PELAQIANGMEKLNWHVPIIGSWPLSMG----NFIDNSGKNGN---GARMPQ 276 Query: 290 NYFESVKNPVNTAFIKKWKAYAKAKGLPNADTVVTNDPMEATYVGIYMWKQAVEKAKTTD 349 + + P AFI+ ++ K +P+A + Y I + A+++A TTD Sbjct: 277 TFIQDGNTPKRKAFIEAYQKAYKVDRMPSAVSAAQG------YDSILLLAAAIKQAGTTD 330 Query: 350 TDKVIAAMGGQTFKAPSGYTLEMDK----TNHHLHKPVMI--GEIK 389 DKV AA+ K G DK +H KP M+ GE+K Sbjct: 331 GDKVRAALENLNTKV-EGVVTTYDKPFTHDDHEAIKPAMVVMGEVK 375 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 91/356 (25%), Positives = 146/356 (41%), Gaps = 42/356 (11%) Query: 29 ADTIKVGILHSLSGTMAISETSLKDVALMTIDEINANGGVNGKKLEPVIVDPASNWPLFA 88 A+T+K+ I ++G +A +K AL I++INA GG NG K E V++D A Sbjct: 26 AETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAV 85 Query: 89 EKARQLISQDKVSVVFGCWTSVSRKSVLPVFKELNSLLFYPV----QYEGEELEKNVFYT 144 A +++SQ + V G S S +++ ++ P Q + K +F T Sbjct: 86 AVANKIVSQG-IKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRT 144 Query: 145 GAAPNQQAIPATEYLMSKEGGGAKRFVLLGTDYVYPRTTNKILRAFLHSKGVKDSDIDEV 204 +QQ A Y++ K K+ +L Y + ++A L + V + + Sbjct: 145 IGRDDQQGPAAARYVIEKL--KPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGI 202 Query: 205 YTPFGHSDYQTIVANIKKFAA-----GGKTAVISTINGDSNVPFYKELGNAGLKATDVPV 259 G SDY IV +K GG ++ E+G +A + + Sbjct: 203 NA--GDSDYSAIVTKLKSQGVDFVYFGG---------------YHPEMGLIMRQAREQGI 245 Query: 260 VAFSVGEEELRGVDAXGVDAKPLVGHLAAWNYFESVKNPVNTAFIKKWKAYAKAKGLPNA 319 A +G E + D + G L + +P N A KA+A AK PN Sbjct: 246 KAVFMGPEGVGNKDITAIAGPASEGMLVTLPA-DFAADPANAA---LVKAFAAAKRDPNG 301 Query: 320 DTVVTNDPMEA-TYVGIYMWKQAVEKAKTTDTDKVIAAMGGQTFKAPSGYTLEMDK 374 P + Y G+ + A+ AK+TD +KV A + +FK P G +E DK Sbjct: 302 -------PFQMPAYSGVKIIADAIAGAKSTDPEKVAAYIHANSFKTPIG-NVEYDK 349 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 80/318 (25%), Positives = 128/318 (40%), Gaps = 62/318 (19%) Query: 17 LAASSWLPAAFAADTIKVGILHSLSGTMAISETSLKDVALMTIDEINANGGVNGKKLEPV 76 L ++ AA AAD IK+G+ +L+G A ++K+ + +EINA GGVNG KL V Sbjct: 14 LIGATLSQAALAAD-IKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDKLALV 72 Query: 77 IVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFKELNSLLF--------- 127 I D ++LI QDKV VFG S S + P+ ++F Sbjct: 73 IEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADGI 132 Query: 128 ---YPVQYEGEELEKNVFYTGAAPNQQAIPATEYLMSKEGGGAKRFVLLGTDYVYPRTTN 184 P + +E +V +P T K G K V+ G D + ++ Sbjct: 133 TAMGPFTFRNSVMEADV-----------LPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGY 181 Query: 185 KILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFAAGGKTAVISTINGD--SNV- 241 + +A L + + + E Y G D++ A + K AG A++ + + +N+ Sbjct: 182 DVFKATLEHQKIPVT-TTEAYAK-GDVDFK---AQLTKIKAGNPDAIVCSCLAEEAANII 236 Query: 242 ----------PFYKELGNAGLKATDVPVVAFSVGEEELRGVDAXGVDAKPLVGHLAAWNY 291 PF +G GL + + +A G+ L G + W+ Sbjct: 237 LQTRALGMKQPF---IGGNGLNSPKLFEIAKDAGDNTLMG---------------SPWS- 277 Query: 292 FESVKNPVNTAFIKKWKA 309 + P N AFI +KA Sbjct: 278 -AENQTPANKAFITAYKA 294 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,231,506 Number of extensions: 51529 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 7 Length of query: 422 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 334 Effective length of database: 1,203,190 Effective search space: 401865460 Effective search space used: 401865460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)