BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0035.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 505 e-144 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 240 1e-64 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 176 2e-45 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 145 4e-36 gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid... 139 4e-34 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 505 bits (1300), Expect = e-144, Method: Compositional matrix adjust. Identities = 251/319 (78%), Positives = 278/319 (87%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGASLPIGNDM 60 MK HVVVYKKLP+ L+QRL+ EF++T F+ + NRA F+AALATA GM+GASLPI N M Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAALATAHGMIGASLPITNAM 60 Query: 61 LEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVVE 120 LEAAP LKIVST+SVGVDNFDL+YFR+RGLMLAHTPGVL EATAD IF+LILASARRVVE Sbjct: 61 LEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVE 120 Query: 121 LAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRPN 180 LAE+VKAG+WKGSIGE+ FGVNVHGKTLG++GMGRIGSAVARRAHHGFGM I+YHN RP+ Sbjct: 121 LAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPD 180 Query: 181 PEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRGRI 240 PEAE L A YVS+++L QADFVC MLPLT QTER GA EF LMK AIFINASRGRI Sbjct: 181 PEAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRI 240 Query: 241 VDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMAEL 300 VDE+ALI ALQ KTI+GAGLDVFE EPLP+ SPLL+LPNVVALPHIGSATHETR AMAEL Sbjct: 241 VDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMAEL 300 Query: 301 AVDNLIAGLRGAQPAHLAV 319 AV NLIAGLRG AHLAV Sbjct: 301 AVTNLIAGLRGEPVAHLAV 319 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 240 bits (613), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 147/324 (45%), Positives = 194/324 (59%), Gaps = 13/324 (4%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAAL-ATAQGMLG----ASLP 55 MK + V + + ++L RL FEV N +R A L A AQG G S P Sbjct: 1 MKPKIFVARAVFPEVLARLEQHFEVES----NQDDRIYSAAELQAKAQGKDGLFTTPSEP 56 Query: 56 IGNDMLEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASA 115 + + A P LK V ++VG +N D+ + G+M +TP VLNE TAD ++L++A+A Sbjct: 57 VTAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAA 116 Query: 116 RRVVELAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYH 175 RR+ E F++AGKWK +S G ++HG TLG++GMGRIG A+ARR+ GF M+++YH Sbjct: 117 RRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRS-LGFDMQVLYH 175 Query: 176 N-TRPNPEAEALLA-ATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFI 233 N +R PE EA A YVS++EL +QAD V +LP + T TI A E LMKP A Sbjct: 176 NRSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLT 235 Query: 234 NASRGRIVDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHET 293 N +RG IVD++ALI AL+ I AG+DVFE EP L L NVV PHI SA+ T Sbjct: 236 NIARGGIVDDAALIAALREGRIAAAGVDVFENEP-AFKPEFLDLSNVVLTPHIASASTPT 294 Query: 294 RCAMAELAVDNLIAGLRGAQPAHL 317 R AMA A DNLIA L G +P +L Sbjct: 295 RLAMANCAADNLIAALSGQRPPNL 318 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 176 bits (446), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 121/314 (38%), Positives = 169/314 (53%), Gaps = 14/314 (4%) Query: 2 KQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGAS--LPIGND 59 + HV++ +LP LL L+ + T + + LAA+A + A+ + + Sbjct: 6 RPHVLIVARLPQHLLDLLQQHY--TCHNLILQPHSEAELAAIAPQIRGIAANGEAKVSRE 63 Query: 60 MLEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVV 119 + P L+IVS VG D D+ R+RG+ + HTP VLN+ AD +L+LA+AR VV Sbjct: 64 FMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNVV 123 Query: 120 ELAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRP 179 F ++G+WK G F V G LG++G+GRIG A+A+RA F M+I YHN Sbjct: 124 RADRFARSGEWKK--GPFPFTTKVSGARLGIVGLGRIGQAIAKRA-AAFDMQISYHN--- 177 Query: 180 NPEAEALLAATYV-SRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRG 238 + + TYV S L ++ DF+ + P A T + A + P IN +RG Sbjct: 178 --RSRKDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 Query: 239 RIVDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMA 298 +VDE+ALI AL+ I GAGLDVF EP V + L L NVV PH+ S T TR AMA Sbjct: 236 SVVDEAALIAALKTGVIAGAGLDVFADEP-NVPAELAALDNVVLTPHMASGTLVTRTAMA 294 Query: 299 ELAVDNLIAGLRGA 312 +LA +NL A GA Sbjct: 295 DLAFNNLQAHFSGA 308 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 145 bits (366), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 94/251 (37%), Positives = 140/251 (55%), Gaps = 9/251 (3%) Query: 60 MLEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVV 119 +++AAP+L+++S G+D+ D + ++RG+ + G A A+ ++LI+A A+ VV Sbjct: 64 VMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAKNVV 123 Query: 120 ELAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRP 179 L + ++ G W S +SL + G+TLG++G+G IG VA A GM ++ H+ Sbjct: 124 GLDQRMREGHWDKSTHKSL---ELQGRTLGLVGLGAIGRRVAAVAAA-LGMPVLAHD--- 176 Query: 180 NPEA-EALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRG 238 P A EA V D LF Q+D V PLTA+ + I A+ MK GAI +N +RG Sbjct: 177 -PYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARG 235 Query: 239 RIVDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMA 298 ++DE ALI AL + AGLD FE+EP PL ++ N V PHIG T + AM Sbjct: 236 GLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTSDAYIAMG 295 Query: 299 ELAVDNLIAGL 309 A N++A L Sbjct: 296 TGAASNVLAVL 306 >gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 348 Score = 139 bits (349), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 113/325 (34%), Positives = 166/325 (51%), Gaps = 25/325 (7%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTY-----FEAVNAGNRAQFLAALATAQGML-GASL 54 MK+HV+ +L L+ + D+ E Y FE + A A +G++ G Sbjct: 32 MKRHVL---QLNPILVPAINDKLESLYHMHRLFEQED--KEAYIARHGAVIEGVITGGHT 86 Query: 55 PIGNDMLEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILAS 114 I N ++++ P LK+V+ VG D DL + R +GL + T G L E AD L+LA Sbjct: 87 GISNALMDSLPALKVVAVNGVGTDAVDLNHARSKGLPVTGTFGALTEDVADLALGLMLAV 146 Query: 115 ARRVVELAEFVKAGKWKGSIGESLFGVN--VHGKTLGMLGMGRIGSAVARRAHHGFGMKI 172 +R++ FV+ G W + ++ + GK +G++GMG++G A+A+RA FG I Sbjct: 147 SRQICPGHAFVQRGDWVRHPSPTAIPLSRRLSGKRVGIVGMGKVGRAIAQRA-AAFGCPI 205 Query: 173 VYHNTRPNPEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERT---IGAREFGLMKPG 229 Y + R A+ Y + EL Q A +L L A ++ I A + Sbjct: 206 TYTDLR------AMEDLPYAFQPELLQLARE-SDVLVLAAAADKAQGIINAAVLDALGRE 258 Query: 230 AIFINASRGRIVDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSA 289 I IN +RG++V E L+DALQR I GAGLDVF EP V + LLQ+ VV H SA Sbjct: 259 GILINIARGKLVVEEDLVDALQRGVIAGAGLDVFVDEP-NVPAALLQMDQVVLQAHRASA 317 Query: 290 THETRCAMAELAVDNLIAGLRGAQP 314 T E+R AM E+ + +L GL G +P Sbjct: 318 TVESRTAMGEMVLASLAQGLAGQRP 342 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 994,494 Number of extensions: 41553 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 11 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)