BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0018.0018.p
         (547 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310805|ref|YP_003774897.1| major facilitator superfamily p...   942   0.0  

>gi|300310805|ref|YP_003774897.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 580

 Score =  942 bits (2436), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 474/548 (86%), Positives = 510/548 (93%), Gaps = 1/548 (0%)

Query: 1   MAFDFLQKEHTIAKPGFNRWLVPPAALAIHLCIGMAYGFSVFWLPLSRALGIKESIACAP 60
           MAFDFL KEHT+AKPGFNRWLVPPAALAIHLCIGMAYGFSVFWLPLS+ALG+   IAC  
Sbjct: 33  MAFDFLNKEHTVAKPGFNRWLVPPAALAIHLCIGMAYGFSVFWLPLSKALGVTAPIACGA 92

Query: 61  DTGFFAQVFSTTCDWKISMLGWMYTMFFVLLGISAAIWGGWLERAGPRKAGVVSALCWCG 120
           D GFFAQVFS++CDWKISMLGWMYTMFFVLLGISAA+WGGWLE AGPRKAGVV+A+CWCG
Sbjct: 93  DVGFFAQVFSSSCDWKISMLGWMYTMFFVLLGISAALWGGWLEHAGPRKAGVVAAICWCG 152

Query: 121 GLLISALGVYTHQIWLMWLGSGVIGGIGLGLGYISPVSTLIKWFPDRRGMATGMAIMGFG 180
           GLLISALGV THQIWLMWLGSGVIGG+GLGLGYISPVSTLIKWFPDRRGMATGMAIMGFG
Sbjct: 153 GLLISALGVKTHQIWLMWLGSGVIGGVGLGLGYISPVSTLIKWFPDRRGMATGMAIMGFG 212

Query: 181 GGALVGSPLADKLMKHFATPTSVGVWETFVAMAVIYFVFMMAGALGYRVPPSGWKPAGWT 240
           GGALVG+PLAD  MK FATPTSVGVWETFV MAVIYFVFMMAGA GYRVPP+GWKPAGWT
Sbjct: 213 GGALVGTPLADAAMKKFATPTSVGVWETFVVMAVIYFVFMMAGAFGYRVPPTGWKPAGWT 272

Query: 241 PP-ATTNSTMITQNHVHVSKVWGIPQFWLVWLVLCLNVSAGIGVIGISSPMLQEVFGGHL 299
           PP A +++TMITQNHVHVSKVWGIPQFWLVWLVLCLNVSAGIGVIGI+SPMLQEVFGG L
Sbjct: 273 PPVAKSSATMITQNHVHVSKVWGIPQFWLVWLVLCLNVSAGIGVIGIASPMLQEVFGGKL 332

Query: 300 LGVNVGFSDLSIEQKAAIAAIAAGFTGLISLFNIGGRFVWASCSDFFGRKMTYVIFFVLG 359
           LGVN+GF+DLS+EQK AIAA+AAGFTGLISLFNIGGRF WASCSDFFGRKMTY IFFVLG
Sbjct: 333 LGVNIGFNDLSVEQKTAIAALAAGFTGLISLFNIGGRFAWASCSDFFGRKMTYAIFFVLG 392

Query: 360 FILYVSLPWSAKAGSIALFVGAVCIILSMYGGGFSTVPAYLADLFGTQMVGAIHGRLLTA 419
           F+LYVSLPWSAKAG+I LFVGAVCIILSMYGGGF+TVPAYLADLFGTQMVGAIHGRLLTA
Sbjct: 393 FVLYVSLPWSAKAGNIPLFVGAVCIILSMYGGGFATVPAYLADLFGTQMVGAIHGRLLTA 452

Query: 420 WATAGILGPVVVNYMREYQLSLGMPREQVYNTTMYILAGMLVVGLICNLLVRPVAAKHFM 479
           WATAGILGPVVVNYMREYQL+ GMP+ +VYNTTMYILAGML++GLICNLL+RPVAAKHFM
Sbjct: 453 WATAGILGPVVVNYMREYQLAQGMPKNEVYNTTMYILAGMLILGLICNLLIRPVAAKHFM 512

Query: 480 TPEELAREKQLAHEKSSSDNTTSLSPTDMALVGRGGNPALIGAAWLAVGIPLIWGISLTL 539
           TPEELA EK+LAHEKS++D   +    DM+ +G GGNP  IG AW AVGIPLIWGI+L L
Sbjct: 513 TPEELAHEKKLAHEKSAADAAVTAGSGDMSQIGSGGNPVFIGLAWAAVGIPLIWGITLVL 572

Query: 540 QKTAVLFR 547
           QK+AVLFR
Sbjct: 573 QKSAVLFR 580


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.141    0.461 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,867,531
Number of extensions: 82368
Number of successful extensions: 384
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 385
Number of HSP's successfully gapped: 1
Length of query: 547
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 457
Effective length of database: 1,193,720
Effective search space: 545530040
Effective search space used: 545530040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 133 (55.8 bits)