BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0015.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbasp... 655 0.0 gi|300312594|ref|YP_003776686.1| 3-isopropylmalate dehydrogenase... 174 1e-44 gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herba... 116 3e-27 >gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 655 bits (1690), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/359 (87%), Positives = 335/359 (93%), Gaps = 1/359 (0%) Query: 1 MTK-HKVAVIAGDGIGQEVIPEGLRAVDAAAKKFGIEIEYTHFDWAHCGYYAKHGKMMPD 59 M+K H++AVIAGDGIGQEV+PEGLRAV AA+KKFGI+IE+THFDWAHC YYA+HGKMMPD Sbjct: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVQAASKKFGIDIEFTHFDWAHCNYYAEHGKMMPD 60 Query: 60 DWFDQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 119 DWF+QLKGFEAI+FGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA Sbjct: 61 DWFEQLKGFEAIYFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 Query: 120 GRKPGDIDFYVVRENTEGEYSSIGGRMYEGTDREIVIQESVFTRHGVDRILKFAYDLAQS 179 RKPGDIDFYVVRENTEGEYSSIGGRMYEGT+RE VIQESVF+R G DRILKFA+DLAQS Sbjct: 121 NRKPGDIDFYVVRENTEGEYSSIGGRMYEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 Query: 180 RPKKHLTSATKSNGIAITMPYWDERVEAMGKNYGDVKTDKYHIDILTAHFVLNPQRFDVV 239 RPKKHLTSATKSNGIAITMPYWDERVEAMG Y DV+ DKYHIDILTAHFVLNP RFDVV Sbjct: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 Query: 240 VAPNLFGDILSDLGPACAGTIGIAPTANLNPDRKLPSLFEPVHGSAPDIAGKNIANPVAT 299 VA NLFGDILSDLGPACAGTIGIAP+AN+NP R+LPSLFEPVHGSAPDI GKNIANPVA Sbjct: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAM 300 Query: 300 IWSGAMMLDFLGNGNPKYKQAHDAILAAIETVLVAGPKTPDLGGSGDTTSVGKAIAAAI 358 IWSGAMMLDFLG+G KY+QAHDA+LAAIETVLV GP TPDLGG +T VG+AIAAAI Sbjct: 301 IWSGAMMLDFLGDGQGKYRQAHDAMLAAIETVLVNGPTTPDLGGKSNTAEVGQAIAAAI 359 >gi|300312594|ref|YP_003776686.1| 3-isopropylmalate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 174 bits (440), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 132/373 (35%), Positives = 188/373 (50%), Gaps = 38/373 (10%) Query: 5 KVAVIAGDGIGQEVIPEGLRAVDAAAKKFGIEIEYTHFDWAHCGYYAKHGKMMPDDWFDQ 64 K+A++ GDGIG E++ E +R ++A +KF +E GY A HG +PD Sbjct: 2 KIAILPGDGIGPEIVNEAVRVLNALDEKFEMETAPV----GGAGYEA-HGHPLPDGTLKL 56 Query: 65 LKGFEAIFFGAVG-WP------ATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGV--P 115 K +AI FGAVG W A P+ ++L R+ L+ N RP P + Sbjct: 57 AKEADAILFGAVGDWKYDKLERALRPEQ-----AILGLRKHLQLFANFRPAICYPELTGA 111 Query: 116 CPLAGRKPGDIDFYVVRENTEGEYSSIGGRMYEGTD------REIVIQESVFTRHGVDRI 169 L ++D +VRE Y M E D RE ++ + RI Sbjct: 112 SSLKPELVANLDILIVRELNGDIYFGQPRGMREAPDGPFKGARE-GFDTMRYSEPEIRRI 170 Query: 170 LKFAYDLAQSRPKKHLTSATKSNGIAITMPYWDERVEAMGKNYGDVKTDKYHIDILTAHF 229 A+ A R K+ L S K+N + T +W + V +GK Y DV+ ++D Sbjct: 171 AHVAFQAAAKRGKR-LCSVDKAN-VLETFQFWKDIVTEVGKEYPDVELSHMYVDNAAMQL 228 Query: 230 VLNPQRFDVVVAPNLFGDILSDLGPACAGTIGIAPTANLNPDRKLPSLFEPVHGSAPDIA 289 V P+ FDV+V N+FGDILSD G+IG+ P+A+L+ + K L+EP HGSAPDIA Sbjct: 229 VKAPKNFDVIVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNK--GLYEPSHGSAPDIA 286 Query: 290 GKNIANPVATIWSGAMMLDFLGNGNPKYKQAHDAILAAIETVLVAGPKTPDLGGSG---- 345 G+ IANP+ATI S AMML F N K +QA D I A++ VL G +T D+ G Sbjct: 287 GRGIANPLATILSAAMMLRFSLN---KAEQA-DRIENAVKKVLAQGLRTADIYEEGCTKV 342 Query: 346 DTTSVGKAIAAAI 358 +T +G+A+ A+ Sbjct: 343 NTQQMGEAVVKAL 355 >gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 418 Score = 116 bits (290), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 106/374 (28%), Positives = 167/374 (44%), Gaps = 51/374 (13%) Query: 9 IAGDGIGQEVIPEGLRAVDAAAKKFGIEIEYTHFDWAHCGYYAKHGKMMPDDWF-DQLKG 67 I GDG+G +V P L+ V+AA +K H+ + G A PD W ++ Sbjct: 34 IVGDGVGVDVTPVMLKVVNAAVEKAYGGARKIHWMEIYAGEKATR-LYGPDVWLPEETLA 92 Query: 68 FEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLAGRKPGDID 127 + A+ P + P + + R+ DLYV LRPVR GVP PL R+P D Sbjct: 93 VLKKYLVAIKGPLSTPVGGGIRSLNVAMRQQLDLYVCLRPVRYFKGVPSPL--REPEKTD 150 Query: 128 FYVVRENTEGEYSSIG--------GRMYEGTDREIVIQESVF-----------TRHGVDR 168 + REN+E Y+ I ++ + RE+ +++ F +R G +R Sbjct: 151 MVIFRENSEDIYAGIEWAAGTPEVNKLIDLLTREMGVKKLRFPESSALGIKPVSREGTER 210 Query: 169 ILKFAYDLAQSRPKKHLTSATKSNGIAITM-PYWDERVEAMGKNYG-------------D 214 +++ A A K +T K N + T + D + +G + Sbjct: 211 LVRQAIQYAIDHDKPSVTLVHKGNIMKFTEGAFRDWGYALAAREFGAELIDDGPWMRLKN 270 Query: 215 VKTDKYHI--DILTAHF----VLNPQRFDVVVAPNLFGDILSDLGPACAGTIGIAPTANL 268 KT + I D +T F ++ P + V+ NL GD +SD A G IGIAP AN+ Sbjct: 271 PKTGRAIIIKDAITDAFFQQVLMRPAEYSVIATLNLNGDYISDAVAAQVGGIGIAPGANM 330 Query: 269 NPDRKLPSLFEPVHGSAPDIAGKNIANPVATIWSGAMMLDFLGNGNPKYKQAHDAILAAI 328 + ++FE HG+AP AGK+ NP ++I S MML +G + +A D I++A+ Sbjct: 331 SDS---VAVFEATHGTAPKYAGKDYVNPGSSILSAEMMLRHMG-----WIEAADLIISAM 382 Query: 329 ETVLVAGPKTPDLG 342 + + + T D Sbjct: 383 QKSVSSKRVTYDFA 396 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,269,397 Number of extensions: 58305 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 3 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)