BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0004.p (224 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family prote... 196 9e-52 >gi|300313000|ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] Length = 586 Score = 196 bits (499), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 103/167 (61%), Positives = 117/167 (70%) Query: 29 PGARFALYLQDTISFAAALFGAWQAGKTIYLPSDTLAETCKALALEVDGFIGEFDAALQP 88 PG R AL+ DT+ FAAALF W AGKT YLPSD L +TC+ L EVD F+GEF A Sbjct: 51 PGQRMALFHHDTLQFAAALFAIWLAGKTAYLPSDALPDTCRQLQGEVDAFLGEFGAHCPA 110 Query: 89 LQPSSAPAVADKAGTPQPLDGDAPALVVYTSGSTGTAQAIPKKLSQLSTEVATLEALFGA 148 L P+ A D L D LVVYTSGSTG AQAIPK+L Q++ EVATLEALFGA Sbjct: 111 LHPAGDAAEGDTPDAWPTLRADFGGLVVYTSGSTGLAQAIPKQLGQMANEVATLEALFGA 170 Query: 149 QAGAADIVSTVSHQHIYGLLFKVLWPLAAGRAIHARSAFFPEDLAAM 195 G A++++TVSHQHIYGLLFKVLW L AGR +HA SAFFPEDL M Sbjct: 171 IVGEAEVIATVSHQHIYGLLFKVLWALCAGRPVHAGSAFFPEDLQRM 217 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 680,156 Number of extensions: 28886 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 1 Length of query: 224 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 142 Effective length of database: 1,231,600 Effective search space: 174887200 Effective search space used: 174887200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)