BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0142.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 186 7e-49 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 186 bits (471), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 96/127 (75%), Positives = 109/127 (85%) Query: 1 MSPELRTDDPAVQSELASFAARQSKLTQELSVLREQSAQRQAEVGEAVSRQKSLVSEFDL 60 M +L DPAVQSE A+FAAR SKL QE+SVLREQSAQRQAE+GEA+SRQKS SE +L Sbjct: 149 MPDDLERGDPAVQSEQAAFAARSSKLMQEMSVLREQSAQRQAELGEAISRQKSQASELEL 208 Query: 61 AQRQYKLVHGMFERNAASQLEDIQAQAKAQDAQSRLSATNAMIPRLTAAIAEAQAKMGEA 120 AQRQYKL+H M RNAASQLEDIQA ++ QDAQS+L+ATNAMIPRL AAIAEAQAK GEA Sbjct: 209 AQRQYKLIHDMLVRNAASQLEDIQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEA 268 Query: 121 SSRFRAD 127 +SRFR+D Sbjct: 269 ASRFRSD 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.122 0.308 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 241,446 Number of extensions: 6209 Number of successful extensions: 78 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 1 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)