BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0132.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 400 e-113 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 122 2e-29 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 118 5e-28 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 112 2e-26 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 110 1e-25 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 400 bits (1027), Expect = e-113, Method: Compositional matrix adjust. Identities = 204/250 (81%), Positives = 223/250 (89%), Gaps = 1/250 (0%) Query: 1 MGQPVIIVTGGSRGIGAAVAQSAAARGYIDXAISYVSNRAAADAVVEGIVKAGGRAIAVK 60 M VI+VTGGSRGIGAA A A RGY A++YV+NRAAADAVV I AGGRAIAV Sbjct: 3 MADKVILVTGGSRGIGAATALLAGQRGYA-VAVNYVTNRAAADAVVAQIQAAGGRAIAVG 61 Query: 61 SDVAVEADILHLFQTVDKELGTLAALVNNAGILEHQSRFDELSADRINRVLLTNVTGSLL 120 DVA E D+L LF TVD ELG + ALVNNAG+L+HQSR +++SA+RINRVL+ NVTGSLL Sbjct: 62 GDVAREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLL 121 Query: 121 CAREAVRRMSTRHGGKGGAIVNLSSMAARLGGPGEYVDYAASKGAIDAFTIGLAKEVAAE 180 CAREAV+RMSTRHGG+GGAIVNLSSMAA+LGGPGEYVDYAASKGAIDAFTIGLAKEVAAE Sbjct: 122 CAREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAE 181 Query: 181 GIRVNAIRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVANAVLWLLSDEASYAT 240 GIRVNA+RPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSA EVANA+LWLLS+EASYAT Sbjct: 182 GIRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYAT 241 Query: 241 GTFIDVSGGR 250 GTFIDVSGGR Sbjct: 242 GTFIDVSGGR 251 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 122 bits (307), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 89/246 (36%), Positives = 134/246 (54%), Gaps = 11/246 (4%) Query: 5 VIIVTGGSRGIGAAVAQSAAARGYIDXAISYVSNRAAADAVVEGIVKAGGRAIAVKSDVA 64 V V GGSRGIGAA+ Q AA G A +YVS+ A A+V + GGRA+A+++D A Sbjct: 19 VAFVQGGSRGIGAAIVQRLAAEGAT-VAFTYVSSPDKAQALVSSVQAKGGRALAIQADSA 77 Query: 65 VEADILHLFQTVDKELGTLAALVNNAGILEHQSRFDELSADRINRVLLTNVTGSLLCARE 124 A + + + G L LVNNAG+L D+ + ++R L NV + +E Sbjct: 78 DAAALQQAIRLAAETFGRLDILVNNAGVLAM-GPLDQFKLEDLDRTLAVNVRSVFVATQE 136 Query: 125 AVRRMSTRHGGKGGAIVNL-SSMAARLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIR 183 A R M G+GG ++++ S+ A R+ G V YA SK A+ T GLA+++ GI Sbjct: 137 AARYM-----GEGGRVISIGSTNADRIPFAGGAV-YAMSKSALVGLTKGLARDLGPRGIT 190 Query: 184 VNAIRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVANAVLWLLSDEASYATGTF 243 VN ++PG + TD++ + G+ L + + R G AEE+A+ V +L E+ Y TG Sbjct: 191 VNNVQPGPVDTDMNPASGDFAA--SLTGLMALPRYGKAEEIASFVAYLAGPESGYITGAN 248 Query: 244 IDVSGG 249 + + GG Sbjct: 249 LMIDGG 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 118 bits (295), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 85/248 (34%), Positives = 130/248 (52%), Gaps = 7/248 (2%) Query: 2 GQPVIIVTGGSRGIGAAVAQSAAARGYIDXAISYVSNRAAADAVVEGIVKAGGRAIAVKS 61 G V +VTGG+ GIGA+++Q AA+G+ N AA + +V AG RA ++ Sbjct: 7 GARVALVTGGAMGIGASISQQLAAKGHTVLVADI--NLDAASKTADALVAAGHRAAPLQM 64 Query: 62 DVAVEADILHLFQTVDKELGTLAALVNNAGILEHQSRFDELSADRINRVLLTNVTGSLLC 121 D+ I F ++K+ G LVNNAG+ S F + + + + NVTG LL Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYS-FLDYPLENWLQTMNVNVTGVLLA 123 Query: 122 AREAVRRMSTRHGGKGGAIVNLSSMAARLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEG 181 + A R M + G+ IVN+SS++ G G Y SK A+ T +A E+A G Sbjct: 124 GQHAARLMVKQQWGR---IVNISSISGIRAGAGR-TAYGTSKAAVIGLTRQMAIELAQYG 179 Query: 182 IRVNAIRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVANAVLWLLSDEASYATG 241 I VN++ PG + T + + + VPM+R G+ +E+A+AV +L ++E++Y TG Sbjct: 180 ITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITG 239 Query: 242 TFIDVSGG 249 I V GG Sbjct: 240 HVIPVDGG 247 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 112 bits (281), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 86/248 (34%), Positives = 137/248 (55%), Gaps = 13/248 (5%) Query: 5 VIIVTGGSRGIGAAVAQSAAARGYIDXAISYVSNRAAADAVVEGIVKAG---GRAIAVKS 61 V +VTG SRGIG A+A A +G I ++ + A A+ E + G GR + Sbjct: 10 VALVTGASRGIGRAIATELARQGA--KVIGTATSESGAAAITEYLAALGPEAGRGAVLNV 67 Query: 62 DVAVEADILHLFQTVDKELGTLAALVNNAGILEHQSRFDELSADRINRVLLTNVTGSLLC 121 + A EA + L + V K+ G+L+ LVNNAGI + Q + + + V+ TN+T Sbjct: 68 NDA-EASV-ALVEQVQKDFGSLSILVNNAGITQDQLAM-RMKDEEWDSVIATNLTAVGRL 124 Query: 122 AREAVRRMSTRHGGKGGAIVNLSSMAARLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEG 181 +R +R M G+ I+N++S+ G PG+ ++YAA+K + + LA+E+ + Sbjct: 125 SRAVLRGMMKARTGR---IINITSVVGEAGNPGQ-MNYAAAKAGVAGMSRALAREIGSRN 180 Query: 182 IRVNAIRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVANAVLWLLSDEASYATG 241 I VN + PG I TD+ + E L+ +P+ R G+AEE+A AV +L S +A+Y TG Sbjct: 181 ITVNCVAPGFIDTDMTRALNEQQSAAILQQ-IPLGRLGAAEEIAYAVAFLASPQAAYITG 239 Query: 242 TFIDVSGG 249 T ++V+GG Sbjct: 240 TTLNVNGG 247 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 80/250 (32%), Positives = 128/250 (51%), Gaps = 9/250 (3%) Query: 1 MGQPVIIVTGGSRGIGAAVAQSAAARGYIDXAISYVSNRAAADAVVEGIVKAGGRAIAVK 60 M + + VTGG GIG A+ GY A N D + + + G A + Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVA-GCGPNSTRKDKWLATMREQGFDIHASE 59 Query: 61 SDVAVEADILHLFQTVDKELGTLAALVNNAGILEHQSRFDELSADRINRVLLTNVTGSLL 120 +V+ F+ V E+G + LVNNAGI +F ++S D + V+ TN+ Sbjct: 60 GNVSNWESTKAAFEKVRAEIGEVDVLVNNAGIT-RDGQFRKMSKDDWDAVIDTNLNSLFN 118 Query: 121 CAREAVRRMSTRHGGKGGAIVNLSSMAARLGGPGEYVDYAASKGAIDAFTIGLAKEVAAE 180 ++ + M R G+ I+N+SS+ + G G+ +Y+ +K I FT+ LA+EVA + Sbjct: 119 VTKQVIEGMCERGFGR---IINISSVNGQKGQFGQ-TNYSTAKAGIHGFTMALAQEVATK 174 Query: 181 GIRVNAIRPGLIYTD-IHASGGEAGRVDRLKDAVPMKRGGSAEEVANAVLWLLSDEASYA 239 G+ VN + PG I TD + A E ++++ +P+KR G EE+A+ V W+ S E YA Sbjct: 175 GVTVNTVSPGYIGTDMVRAIRPEV--LEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYA 232 Query: 240 TGTFIDVSGG 249 TG+ ++GG Sbjct: 233 TGSDFSLNGG 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 782,557 Number of extensions: 32139 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 42 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)