BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0117.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 663 0.0 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 215 7e-57 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 211 1e-55 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 199 3e-52 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 197 1e-51 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 663 bits (1710), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/436 (77%), Positives = 368/436 (84%), Gaps = 4/436 (0%) Query: 6 STTSASATSSVAAGPSTTKQRILAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDTT 65 TT AS S A ++RI AILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGD T Sbjct: 3 DTTQASTPLSAA----EYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPT 58 Query: 66 TQLLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMMISVLMMCGGSLMIAFLPTYAVI 125 +QLLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSM+ISVLMMCGGSL +A +PTYA I Sbjct: 59 SQLLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATI 118 Query: 126 GTAAPFLLLVARLFQGLSVGGEYGTSATYMSEIAPKNNRGFFASFQYVTLIGGQLLAVLV 185 G AP LLL+ARLFQGLSVGGEYGTSATYMSE+AP RGFFASFQYVTLIGGQLLAVLV Sbjct: 119 GAWAPALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLV 178 Query: 186 LVAMQQTLSNEELRAWGWRVPFVLGAFGALIALYLRKSLTETATAETRTKKEAGTFAGLW 245 L MQQ L+ EL AWGWRVPFVLGA GAL+A+YLR SL ET++A R KK+AGT GL Sbjct: 179 LFGMQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLL 238 Query: 246 KHKRAFFTVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDAKTASAVMTGALFVYMILQPVF 305 +HKRAF VVGFTAGGSLMFYTFTTYMQKYLVNTAGMD K A+ VMTGALFVYMILQP+F Sbjct: 239 QHKRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIF 298 Query: 306 GALSDKIGRRNMMLLFGVLGTLCTVPILHMLKDVTDPYAAFGLAILALCIVSFYTSISGL 365 GA+SDKIGRRN ML F G + T PILH LKDV+ P A LAILAL IVSFYTSISGL Sbjct: 299 GAISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGL 358 Query: 366 IKAEMFPPEVRALCVGLSYAVGNALFGGSAEFVALSFKAAGMESNFYWYVSIMCAIALLI 425 IKAEMFPPEVRAL VGLSYAVGNA+FGGSAEFVALS K+AG+ES FYWYVS +C +AL+I Sbjct: 359 IKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALII 418 Query: 426 TFRMRDPSKVGYLKDE 441 + RM DP + G+LK + Sbjct: 419 SLRMPDPQRDGHLKGD 434 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 215 bits (547), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 149/435 (34%), Positives = 212/435 (48%), Gaps = 21/435 (4%) Query: 20 PSTTKQRILAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDTTTQLLNTAGVFAAGF 79 P KQR ++ A GN +EW+DF VY F + A FFP + T LL F GF Sbjct: 3 PQLNKQR--QVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGF 60 Query: 80 LMRPIGGWLFGRIADKHGRKTSMMISVLMMCGGSLMIAFLPTYAVIGTAAPFLLLVARLF 139 MRP+GG L G AD+ GRK +M + +++M +IAF PTYA IG AAP +++VAR+ Sbjct: 61 FMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARML 120 Query: 140 QGLSVGGEYGTSATYMSEIAPKNNRGFFASFQYVTLIGGQLLAVLVLVAM----QQTLSN 195 QG + GGEY +S ++ E AP + RG + S+Q V GQ LAV M + LS Sbjct: 121 QGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLV----GQCLAVFCGAGMGALVTRNLSP 176 Query: 196 EELRAWGWRVPFVLGAFGALIALYLRKSLTETAT-AETRTKKEAGTFAGLWKHK---RAF 251 E L +WGWR+PFVLG + L++R+ + ET E+ K A L K R Sbjct: 177 EALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGV 236 Query: 252 FTVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDAKTASAVMTGALFVYMILQPVFGALSDK 311 +G G++ FY M + G+ A V A+ + + P GALSD+ Sbjct: 237 LVSMGQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDR 296 Query: 312 IGRRNMMLLFGVLGTLCTVPILHMLKDVTDPYAAFGLAILALCIV---SFYTSISGLIKA 368 +GRR +LL+ L L V L + + + LCIV +F + L + Sbjct: 297 LGRRT-VLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTAL--S 353 Query: 369 EMFPPEVRALCVGLSYAVGNALFGGSAEFVALSF-KAAGMESNFYWYVSIMCAIALLITF 427 E FP VR+ + ++Y + LFGG A F+ K +G WYV I + + Sbjct: 354 EQFPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAW 413 Query: 428 RMRDPSKVGYLKDED 442 MRD S + + Sbjct: 414 AMRDQSPAAKNRQTE 428 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 211 bits (536), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 130/392 (33%), Positives = 204/392 (52%), Gaps = 12/392 (3%) Query: 14 SSVAAGPS---TTKQRILAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDTTTQLLN 70 SS A P T Q AI+ +S GN +EWFD +Y A+ A FFP GD + LL Sbjct: 2 SSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLL 61 Query: 71 TAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMMISVLMMCGGSLMIAFLPTYAVIGTAAP 130 T F F MRP+G + G +D+ GRK ++M+S+++M G+ MIAF+P+YA IG AP Sbjct: 62 TFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAP 121 Query: 131 FLLLVARLFQGLSVGGEYGTSATYMSEIAPKNNRGFFASFQYVTLIGGQLLAVLVLVAMQ 190 + + ++ QG S GGE+G+S ++ E AP + RGFF+S+Q + LLA L + Sbjct: 122 AGIALGKMIQGFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 191 QTLSNEELRAWGWRVPFVLGAFGALIALYLRKSLTETATAETRTKKEAGTFAGLWKHKRA 250 L+ E+L +WGWRVPF+ G A +Y+R+ L E + ++K + H++ Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM 240 Query: 251 FFTVVGFTAGGSLMFYTFTTYMQKYL----VNTAGMDAKTASAVMTGALFVYMILQPVFG 306 + GS++ T + Y+ Y+ V G+ A ++ A M+ A + I P+ G Sbjct: 241 RLLI----GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVG 296 Query: 307 ALSDKIGRRNMMLLFGVLGTLCTVPILHMLKDVTDPYAAFGLAILALCIVSFYTSISGLI 366 ALSDKIGR M+ L + T P+ L + L + +++ Y + + Sbjct: 297 ALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPAL 356 Query: 367 KAEMFPPEVRALCVGLSYAVGNALFGGSAEFV 398 A++FP R + L+Y + +FGG A + Sbjct: 357 LADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 199 bits (507), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 126/384 (32%), Positives = 199/384 (51%), Gaps = 14/384 (3%) Query: 24 KQRILAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDTTTQLLNTAGVFAAGFLMRP 83 ++ +L +SGN +E FDF++Y F A Y + FFP + L+ T G F AGFLMRP Sbjct: 10 HSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMRP 69 Query: 84 IGGWLFGRIADKHGRKTSMMISVLMMCGGSLMIAFLPTYAVIGTAAPFLLLVARLFQGLS 143 +G + G D+ GR+ +++++ +M G+L+IAF+P YA IG AAP L+L RL QG S Sbjct: 70 LGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFS 129 Query: 144 VGGEYGTSATYMSEIAPKNNRGFFASFQYVTLIGGQLLAVLVLVAMQQTLSNEELRAWGW 203 G E G + Y++E+A N+GF+ S+Q + + + L+ + Q+LS ++ WGW Sbjct: 130 AGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGW 189 Query: 204 RVPFVLGAFGALIALYLRKSLTETATAETRTKKEA-----GTFAGLWKHKRAFFTVVGFT 258 R+PF +G +R+SL ET R + + + A WK A +V T Sbjct: 190 RIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGMMMVVMT 249 Query: 259 AGGSLMFYTFTTYMQKYLVNTAGMDAKTASAVMTGALFVYMILQPVFGALSDKIGRRNMM 318 ++ FY T Y + N + A + V P+ GALSD++GRR ++ Sbjct: 250 ---TVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVL 306 Query: 319 LLFGVLGTLCTVPILHMLKDVTDPYAAFGLAILALCIVSFYTSISG---LIKAEMFPPEV 375 + F +L L P++ L V+D + L I L + Y S +G + E+ P V Sbjct: 307 VAFTLLTLLTAYPVMSWL--VSD-ISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHV 363 Query: 376 RALCVGLSYAVGNALFGGSAEFVA 399 R + L+Y++ A+FGG V+ Sbjct: 364 RTVGFSLAYSLATAIFGGFTPLVS 387 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 197 bits (501), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 125/386 (32%), Positives = 206/386 (53%), Gaps = 24/386 (6%) Query: 21 STTKQRILAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDTTTQLLNTAGVFAAGFL 80 + ++ + +L +SGN +E FDF+++ F A + + AFFP + L+ T F AGFL Sbjct: 5 TASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFL 64 Query: 81 MRPIGGWLFGRIADKHGRKTSMMISVLMMCGGSLMIAFLPTYAVIGTAAPFLLLVARLFQ 140 MRP+G + G D+ GR+ +++++ +M G+++IAF+P+YA IG AP L+L+ RL Q Sbjct: 65 MRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQ 124 Query: 141 GLSVGGEYGTSATYMSEIAPKNNRGFFASFQYVTLIGGQLLAVLVLVA----MQQTLSNE 196 G S G E G + Y++E+A N+GF+ S+Q + Q +A++V A + +L+ Sbjct: 125 GFSAGVELGGVSVYLAEMATPGNKGFYVSWQSAS----QQVAIIVAAAIGYLLSTSLTPA 180 Query: 197 ELRAWGWRVPFVLGAFGALIALYLRKSLTETATAETRTKKEA--GTFAGL---WKHKRAF 251 E+ WGWR+PF +G + +R+SL ET R + + F + WK A Sbjct: 181 EVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAG 240 Query: 252 FTVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDAKTASAVMTGALFVYMILQPVFGALSDK 311 +V T ++ FY T Y + N + + + V I P+ GALSD+ Sbjct: 241 MMLVSMT---TVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDR 297 Query: 312 IGRRNMMLLFGVLGTLCTVPILHMLKDVTDPYAAFG-LAILALCIVSFYTSISGLIKA-- 368 +GRR +++ F VL L P ++ L V D A+F + I+ L + Y S +G + Sbjct: 298 VGRRPLLVTFTVLTILTAYPAVNWL--VHD--ASFAKMLIVELWLSFLYASYNGAMVVAL 353 Query: 369 -EMFPPEVRALCVGLSYAVGNALFGG 393 E+ P +VR L+Y++ A+FGG Sbjct: 354 TEVMPADVRTAGFSLAYSLATAVFGG 379 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,208,953 Number of extensions: 47018 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 273 Number of HSP's successfully gapped: 16 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)