BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0083.p (532 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 871 0.0 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 150 3e-37 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 134 2e-32 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 122 8e-29 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 117 2e-27 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 871 bits (2250), Expect = 0.0, Method: Compositional matrix adjust. Identities = 435/532 (81%), Positives = 491/532 (92%) Query: 1 MSPYKEAAHSSGQVLPFRESLMATLGICFVIMLVALDQTIVGTALPTIVAELKGFDLYAW 60 M+ + ++AHSSGQVLPFRESL+ATLGICFVIMLVALDQTIVGTALPTIVAELKGF+LYAW Sbjct: 1 MALHTDSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW 60 Query: 61 VATSYLLTSVITVPIFGRLGDYFGRKPFLIASIIIFVIASMLCGMADSMLFLVLARGLQG 120 VAT+YLLTSVITVPIFGRLGDYFGRKPF+IASII+FV AS LCGMA+SMLFLVLARGLQG Sbjct: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 120 Query: 121 IGGGMLVGTAFASAADLFPDPHIRLRWQIILSAAYGIATSVGPSLGGFLTQSLGWRWTFY 180 IGGGMLVGT FAS ADLFPDP +RLRWQIILS ++GIAT+VGPSLGGFLTQ LGWRW FY Sbjct: 121 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 180 Query: 181 VNLPVGLVSLFFAMRYIPHIRHHKNAGKVRLDWLGALLISVALGSLQLLVELFPKRGLTG 240 NLP+G++SLFF R++PHIRH ++AGK+RLDW GALLIS++LGSLQLLVEL PKRG+T Sbjct: 181 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS 240 Query: 241 GMLALLVVAIGAFYALWKWERQVEQPILPFEMFKDPALSSLFLLSVLAGFSMFSMLMYAP 300 GML LL V++ AFYALWKWE++ QPILPFEM +D AL+SLFLLSVLAGF+MFSMLMYAP Sbjct: 241 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAP 300 Query: 301 LLFQGGFGMSPREAGLLITPLVACITVASIVNGRIVTRIPNPNMMMYVGFVLIALSCLGI 360 LLFQGGFG+SPREAGLLITPLVACITVASIVNGRI+TRIPNPN+MM +GF LIA SCLGI Sbjct: 301 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGI 360 Query: 361 VLSQRNTPTGFMLATMLAGGFGLGLVLPNLTVFAQQAASREHLGIATALLQSLRMIGGML 420 VL R T GF+LA MLAGGFGLGLVLPNLTVFAQQAA+REHLGIATALLQSLRMIGGML Sbjct: 361 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 Query: 421 GTAVTGTLVTQMYGSGVERALESDHAAQWLAEFKDPEILVNHDIQSTLLAQMLQAGHNGS 480 GTA+TGTLV+QMYG+GVE+ALE DHAAQWL +F DPE+LVNHDIQS LLAQ++QAGH+GS Sbjct: 421 GTAITGTLVSQMYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS 480 Query: 481 ALLDAARDALVGAIHMGIALAVVSALIGLWMVRRVPLIRFTPNEKVETVLSE 532 ALL+AAR+ALVGAIHMGIA+A+V+AL+GLWMVRRVP IRF ++KVE V+SE Sbjct: 481 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE 532 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 150 bits (378), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 130/427 (30%), Positives = 204/427 (47%), Gaps = 17/427 (3%) Query: 22 MATLGICFVIMLVALDQTIVGTALPTIVAELKG-FDLYAWVATSYLLTSVITVPIFGRLG 80 + L I FV+ + LD T+V L I L W+ Y LT + + G L Sbjct: 32 LVALAIGFVMAM--LDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALA 89 Query: 81 DYFGRKPFLIASIIIFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTAFASAADLFPD 140 D FG + + + +FV+AS+LCG A + FL++AR LQG+G + ++ + ++ D Sbjct: 90 DRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYED 149 Query: 141 PHIRLRWQIILSAAYGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAMRYIPHI 200 +R R + SA G+A +VGP +GG L LGWR F +N+PVGLV L A + IP + Sbjct: 150 DRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQV 209 Query: 201 RHHKNAGKVRLDWLGALLISVALGSLQLLVELFPKRGLTG-GMLALLVVAIGAFYALWKW 259 H A L+ L V L L ++ P G T +LA L VA+ A AL + Sbjct: 210 GGHARA----LNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 260 ERQVEQPILPFEMFKDPALSSLFLLSVLAGFSMFSMLMYAPLLFQGGFGMSPREAGLLIT 319 E + P+LP +F P ++ + L F ++ L Y L Q G G + GL + Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 320 PLVACITVASIVNGRIVT----RIPNPNMMMYVGFVLIALSCLGIVLSQRNTPTGFMLAT 375 P++A I ++++GR+ R+P + + VG V AL+ +G TP + Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPL-LLGLSVGTVFTALA-MGF---SPATPYWLLALV 380 Query: 376 MLAGGFGLGLVLPNLTVFAQQAASREHLGIATALLQSLRMIGGMLGTAVTGTLVTQMYGS 435 + G+ +P +T Q A + H A A L + R IG ++G A+ G ++ + G Sbjct: 381 CASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGW 440 Query: 436 GVERALE 442 + L Sbjct: 441 NLRNPLA 447 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 134 bits (338), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 123/443 (27%), Positives = 201/443 (45%), Gaps = 21/443 (4%) Query: 9 HSSGQVLPFRESLMATLGICFVIMLVALDQTIVGTALPTIVAELK-GFDLYAWVATSYLL 67 H + + LP ++ A L + + D TI+ ALP I L+ + WV+ +YLL Sbjct: 16 HPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLL 75 Query: 68 TSVITVPIFGRLGDYFGRKPFLIASIIIFVIASMLCGMADSMLFLVLARGLQGIGGGMLV 127 + +T+ F L G + A + +F +AS+ C ++ S+ L+L R LQG+GG ++ Sbjct: 76 ATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAML 135 Query: 128 GTAFASAADLFPDPHIRLRWQIILSAAYGIA--TSVGPSLGGFLTQSLGWRWTFYVNLPV 185 A A +FP+ RL +I+ A +A T+VGP LGG L LGW W F +N+P+ Sbjct: 136 SIAPAIHRTIFPN---RLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPL 192 Query: 186 GLVSLFFAMRYIPHIRHHKNAGKVRLDWLGALLISVALGSLQLLVEL---FPKRGLTGGM 242 G+++++ A +P R A D GAL ++A+G+L + + F + G G Sbjct: 193 GVLAVWLAWNAVPDTRKPAQAA---FDLAGALWSALAMGALIMAADTCARFAEPGQGSGA 249 Query: 243 LALLVVAIGAFY----ALWKWERQVEQPILPFEMFKDPALSSLFLLSVLAGFSMFSMLMY 298 A + +R+ P+LP ++F +P SL L+ LA F + Sbjct: 250 AFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRF-SLAALTSLASFVGQGIAFV 308 Query: 299 A-PLLFQGGFGMSPREAGLLITPLVACITVASIVNGRIVTRIPNPNMMMYVGFVLIALSC 357 A P LFQ +G + E+ L TP I + + GR+ R + + Sbjct: 309 ALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLV 368 Query: 358 LGIVLSQRNTPTGFMLATMLAG-GFGLGLVLPNLTVFAQQAASREHLGIATALLQSLRMI 416 L +L + P ++ G GFG N + SRE G A+ +L +R Sbjct: 369 LLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGNASGVLAIMRTF 426 Query: 417 GGMLGTAVTGTLVTQMYGSGVER 439 G LG A+ G ++ + V + Sbjct: 427 GQCLGAALLGVILAVRTAAAVAQ 449 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 122 bits (306), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 123/461 (26%), Positives = 209/461 (45%), Gaps = 28/461 (6%) Query: 36 LDQTIVGTALPTIVAELKGFDLYAWVA-TSYLLTSVITVPIFGRLGDYFGRKPFLIASII 94 +D T++ T+LP + ++ + +A TSYL++ + +PI G + D FG ++I Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 95 IFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTAFASAADLFPDPH-IRLRWQIILSA 153 +F++ S+LCG++ ++ V AR LQGIGG M+V + ++ + + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 154 AYGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAMRYIPHIRHHKNAGKVRLDW 213 +G +GP LGGF+T WRW F++N+P+ ++ L+ A R+IP ++ + A LD Sbjct: 121 LFG--PVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVA---PLDL 175 Query: 214 LGALLISVALGSLQLLVELFPK-RGLTGGMLALLVVAIG-AFYALWKWE-RQVEQPILPF 270 G LL ALG L++ L R L G +AL V G F A + W R+ P++ Sbjct: 176 KGFLL--SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDL 233 Query: 271 EMFKDPALSSLFLLSVLAGFSMFSMLMYAPLLFQGGFGMSPREAGLLITPLVACITVASI 330 + K P L + + ++ PL+ Q GFG +P +G+L A Sbjct: 234 ALLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKT 293 Query: 331 VNGRIVTRIPNPNMMMYVGFVLIALSCLG---IVLSQRNTPTGFMLATMLAGGFGLGLVL 387 + R + R +++ G AL LG I TP M+ +L GG L Sbjct: 294 IAARFLRRYGFRRVLLVNG----ALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQF 349 Query: 388 PNLTVFAQQAASREHLGIATALLQSLRMIGGMLGTAVTGTLVTQMYGSGVERALESDHAA 447 +L + + L AT++ + + +G + G ++ + E A Sbjct: 350 TSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGF--------ALQMSNEIQGHA 401 Query: 448 QWLAEFKDPEILVNHDIQSTLLAQMLQAGHNGSALLDAARD 488 +A+ P LV + + L + Q H+ A + A RD Sbjct: 402 TLVAQDFWPAFLVVGAVAACALLMLRQLPHDAGAEM-AGRD 441 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 117 bits (294), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 90/283 (31%), Positives = 136/283 (48%), Gaps = 18/283 (6%) Query: 36 LDQTIVGTALPTIVAELK-GFDLYAWVATSYLLTSVITVPIFGRLGDYFGRKPFLIASII 94 LD TI+ TALP + G + + T+Y+LT + +PI G + D FG + +I Sbjct: 45 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIA 104 Query: 95 IFVIASMLCGMADSMLFLVLARGLQGIGGGMLVGTA-----FASAADLFPDPHIRLRWQI 149 IF +AS+LCG + ++ AR LQG+GG M+V ++ D + W Sbjct: 105 IFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 164 Query: 150 ILSAAYGIATSVGPSLGGFLTQSLGWRWTFYVNLPVGLVSLFFAMRYIPHIRHHKNAGKV 209 I +A VGPS+GGF+T W W F +N+P GL + M +R+ K Sbjct: 165 I------VAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMV---VRNEHGVSKR 215 Query: 210 RLDWLGALLISVALGSLQLLVELFPKRGLTGGMLALLVVAIGAF--YALWKWERQVEQPI 267 LD +G LL AL SL E+ + +AL VA+G Y W+ +++E P+ Sbjct: 216 PLDLVGFLLSGAALTSLLYGTEI-ASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHPL 274 Query: 268 LPFEMFKDPALSSLFLLSVLAGFSMFSMLMYAPLLFQGGFGMS 310 L F + P S L L + ++ PL+FQ GFG++ Sbjct: 275 LDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLT 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,601,533 Number of extensions: 65799 Number of successful extensions: 472 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 440 Number of HSP's successfully gapped: 20 Length of query: 532 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 442 Effective length of database: 1,193,720 Effective search space: 527624240 Effective search space used: 527624240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)