BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0074.p (767 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxi... 1393 0.0 gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxi... 872 0.0 >gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 770 Score = 1393 bits (3606), Expect = 0.0, Method: Compositional matrix adjust. Identities = 692/767 (90%), Positives = 731/767 (95%) Query: 1 MISKKEELRQQLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVAD 60 MISKKEE+RQQLRQAAL+YHEFPTPGKI+VTPTKLLTNQRDLSLAYSPGVAAACEEIVAD Sbjct: 4 MISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEIVAD 63 Query: 61 PANAFKYTARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINE 120 P NAF+YTARGNLVAVISNGTAVLGLGNIG LASKPVMEGK VLFKKFAGIDVFDIE+NE Sbjct: 64 PLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIEVNE 123 Query: 121 TDPDKLCDIIASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAA 180 TDPDKL D+IA+LEPTFGGINLEDIKAPECFYIERKLR++MKIPVFHDDQHGTAIIVGAA Sbjct: 124 TDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIVGAA 183 Query: 181 ILNGLKVVGKSIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELM 240 ILNGLKVVGK IK VKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGR ELM Sbjct: 184 ILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRKELM 243 Query: 241 DPDKERFARETDARTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEIL 300 DPDKERFARET+ARTLA+VIPDADIFLG+SA GVLK +MVK+MA RPLVLALANPTPEIL Sbjct: 244 DPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTPEIL 303 Query: 301 PEDVMAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADL 360 PE+V AVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIA VHAIA+L Sbjct: 304 PEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAIAEL 363 Query: 361 AQAEQSDIVAATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMS 420 AQAEQSDIVA TYG+ NLSFGPEYIIPKPFDPRLMIKIA AVARAAE+SGVAARPI+DM Sbjct: 364 AQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQDMD 423 Query: 421 AYAEKLEQFVYHSGTFMRPIFQAAKKAPDAKKRIVFAEGEEERILRAVQVIVDECLAKPI 480 AY EKLEQFVYHSGTFMRPIFQ AKKA +AKKRIV+AEGEEER+LRAVQVIVDE LAKPI Sbjct: 424 AYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLAKPI 483 Query: 481 LVGRPEVLEQRIKKFGLRLRPNVDFEVINPNFDSRYRDYWQTFLEMTKRRGVTEQYAKLE 540 LVGRPEVLEQRI+KFGLRLR DFEVINPNFD+RYRDYWQTFLEM++R+GVTEQYAKLE Sbjct: 484 LVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYAKLE 543 Query: 541 MRRRHTLIGSMAIHKGHADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNGVILPSR 600 MRRRHTLIGSMAIHKG ADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMN VILP+R Sbjct: 544 MRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVILPNR 603 Query: 601 QLVLVDTHVNENPTAEQLAEITIMAAEEMRRFGLHPRAALLSHSNFGSSNSESAVKMRTA 660 QLV+VDTHVNENP A +LAEIT++AAEEMRRFGLHPRAALLSHSNFGSSNSESA KMR A Sbjct: 604 QLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKMREA 663 Query: 661 LKLIQEQAPELEADGEMHGDTALDPKLLAQIMPDSPLKGDANLLVMPNIDAANIAYNLLK 720 L +++E APELE DGEMHGDTALD KLL ++PDSPLKGDANLLVMPNIDAANIAYNLLK Sbjct: 664 LAILREIAPELEVDGEMHGDTALDSKLLNAVIPDSPLKGDANLLVMPNIDAANIAYNLLK 723 Query: 721 TASGNGVAIGPILLGCAKPVHILTASATVRRIVNMTALCVMDAMSER 767 TASGNGVAIGPILLGCAKPVHILT SATVRRI+NMTALCVMDA+SER Sbjct: 724 TASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDALSER 770 >gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 758 Score = 872 bits (2252), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/763 (58%), Positives = 559/763 (73%), Gaps = 17/763 (2%) Query: 2 ISKKEELRQQLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVADP 61 IS E+L LR AA EYH P+ GKI V PTK L+NQRDLSLAYSPGVA C +I ADP Sbjct: 5 ISAAEQL---LRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDIEADP 61 Query: 62 ANAFKYTARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINET 121 AF YT+RGNLVAVISNGTAVLGLGNIGPLASKPVMEGK LFKKFAG+DVFDIE+ E Sbjct: 62 LKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAEN 121 Query: 122 DPDKLCDIIASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAAI 181 DPDKL +IIASLEPT GGINLEDIKAPECFYIE+KL RM IPVFHDDQHGTAII AA+ Sbjct: 122 DPDKLVEIIASLEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAAL 181 Query: 182 LNGLKVVGKSIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELMD 241 LNGL++VGK I +VK+ VSGAGAAA+AC+++++ LG EN+Y+ D GV+Y+GR +D Sbjct: 182 LNGLELVGKDIGKVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGLD 241 Query: 242 PDKERFARETDARTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEILP 301 K+R+A +T+ARTLADV+ AD+FLG SA GVL +MVK MA +P++LALANP PEI P Sbjct: 242 ASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLSAEMVKTMADKPIILALANPEPEIRP 301 Query: 302 EDVMAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADLA 361 E +VR D IIATGRSDYPNQVNNVLCFPYIFRGALD GAT IT M++A V IADLA Sbjct: 302 ELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADLA 361 Query: 362 QAEQSDIVAATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMSA 421 +AE S V+A Y L FG +Y+IP PFD RL++KIA AVA+AA SGVA RPIKD A Sbjct: 362 KAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIKDYDA 421 Query: 422 YAEKLEQFVYHSGTFMRPIFQAAKKAPDAKKRIVFAEGEEERILRAVQVIVDECLAKPIL 481 Y +L +F+Y +G FM+P+F AK AP +R+V+A+G++ER+LRAVQ + DE LA PIL Sbjct: 422 YRAELTRFIYQTGMFMQPVFNTAKVAP---QRVVYADGQDERVLRAVQFVADEGLAAPIL 478 Query: 482 VGRPEVLEQRIKKFGLRLRPNVDFEVINPNFDSRYRDYWQTFLEMTKRRGVTEQYAKLEM 541 VG P +E IKK GLR++ + +VI+P D+R Y + + + ++ Sbjct: 479 VGAPAAIEAGIKKAGLRIKIGQNVQVIDPATDARVAQYAAQY----------KDGGEADV 528 Query: 542 RRRHTLIGSMAIHKGHADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNGVILPSRQ 601 R +L+ ++ + G AD M+CG G HL ++++V+G ++G +AA+N V+LP + Sbjct: 529 RASGSLLAALMVKAGDADAMLCGLDGNYDGHLAHVEKVIGLKQGAQEFAALNAVLLPDQT 588 Query: 602 LVLVDTHVNENPTAEQLAEITIMAAEEMRRFGLHPRAALLSHSNFGSSNSESAVKMRTAL 661 L + DT+VN+ P AEQLA I AAEE+ RFG+ P+ A LSHSN GSS+ SA KM A Sbjct: 589 LFITDTYVNDTPDAEQLARIAQAAAEEVARFGVPPKVAFLSHSNRGSSSRPSARKMAAAH 648 Query: 662 KLIQEQAPELEADGEMHGDTALDPKLLAQI-MPDSPLKGDANLLVMPNIDAANIAYNLLK 720 + ++ APE+E+ GE+HGD AL K+ + DS L G AN+LV PN+DAANI +N+LK Sbjct: 649 EAFKKLAPEVESAGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILFNVLK 708 Query: 721 TASGNGVAIGPILLGCAKPVHILTASATVRRIVNMTALCVMDA 763 T+SG G IGPILLG A PVH+LT S+TVRR++NMTAL A Sbjct: 709 TSSGQGTTIGPILLGAAAPVHVLTPSSTVRRVINMTALAAAKA 751 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,270,632 Number of extensions: 93926 Number of successful extensions: 320 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 317 Number of HSP's successfully gapped: 2 Length of query: 767 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 674 Effective length of database: 1,179,515 Effective search space: 794993110 Effective search space used: 794993110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)