BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0065.p (166 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312665|ref|YP_003776757.1| membrane protein [Herbaspirillu... 176 1e-45 >gi|300312665|ref|YP_003776757.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 177 Score = 176 bits (446), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 92/165 (55%), Positives = 116/165 (70%), Gaps = 4/165 (2%) Query: 6 RFLRHLRHTRASARASFPPATLQAIQTCIGAGEAKHRAEVRLVIEAALPLGAVLRNESSR 65 R L HLR TRA+AR +FP TL+AIQ IGAGE +HRA+VR+++EAAL LGAV R +S+R Sbjct: 13 RLLHHLRMTRAAARRAFPSTTLKAIQASIGAGEREHRAQVRVIVEAALSLGAVRRGDSAR 72 Query: 66 ARAHELFSHYRIWDTEENSGVLIYLNLADHRVEIVTDRTVGRVLKRADWESVCSTMTRSF 125 RAHELFSHYRIWDTEEN GVL+Y+NLAD +VEIV DR V + R W+ VC MT + Sbjct: 73 QRAHELFSHYRIWDTEENCGVLVYINLADRKVEIVADRGVNARVTREQWQQVCRAMTAGY 132 Query: 126 ANGIYHDGVLEALAQLNGLLTVHFPQ----TSHQKNEISNRPLML 166 A G Y L+ALA L+ +L P+ NE+S++PL+L Sbjct: 133 AQGEYEASTLQALASLHEILRKVLPREPDAAGDMHNELSDKPLLL 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 481,008 Number of extensions: 17359 Number of successful extensions: 42 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 42 Number of HSP's successfully gapped: 1 Length of query: 166 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 88 Effective length of database: 1,250,540 Effective search space: 110047520 Effective search space used: 110047520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)