BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0048.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 639 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 251 6e-68 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 239 4e-64 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 227 1e-60 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 140 2e-34 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 639 bits (1649), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/444 (74%), Positives = 375/444 (84%), Gaps = 1/444 (0%) Query: 1 LWIVASGFFMQTLDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLAMASLTPASGWLADR 60 LWIVASGFFMQTLDTTIVNTALP+MA SLGEPVL MHPVVV+YTL MASLTPASGWLADR Sbjct: 55 LWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADR 114 Query: 61 FGTRRVYFLAILIFVLGSLLCANAHSLSQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQ 120 FGTRR+YF AIL+F LGSLLCA A SL LL ARVVQG+GGSMLLP+GRLAVLR+VPGAQ Sbjct: 115 FGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQ 174 Query: 121 YISALAFISIAGQLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPAEEIV 180 YI+ALA ISIAGQLGPVVGPV GGWL +++ WHWIFLIN+PVGV+GM AV FLP + + Sbjct: 175 YIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLA 234 Query: 181 SPPRFDMLGCALLSTCMVAFSLALDVPMEEHRAWWSAGLFGISVLTVWWYVVHARRDEQP 240 PRFD+LGC LLS CMVAFSLALDVP + HR WS LF ++ LTV Y HA +P Sbjct: 235 GTPRFDLLGCVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVLGYWRHASVHPRP 294 Query: 241 LFRLGLFNEPNFSVGLIGNLICRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGT 300 LFRLGLF EPNFS+GL GNL+CRIGSSAVPFLLPLLLQV+LG+SPLHAG+VLLPAA+AGT Sbjct: 295 LFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGT 354 Query: 301 IAKPWIAPLVRRYGYENFLLVNTVMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQF 360 IAKPWIAPLV+R+GY+ FLLVNT +VGAAI+SFALITP WP+ IVQLA+FG NSMQF Sbjct: 355 IAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQF 414 Query: 361 AAMNSVTLKGLNSKDAGSGNSLFSMVQMLALGLGVTIAGSMLSGIDG-VAWARDWSFTLT 419 AAMNSVTLKGL+ +DAGSGNSLFSMVQMLA+GLGVTI G+M++ V A + SF +T Sbjct: 415 AAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATNASFQIT 474 Query: 420 FVCVGLVTLLSALVFRRMDARYFN 443 F VG +TL SA VFR+MD RYF Sbjct: 475 FAIVGAITLASAWVFRKMDPRYFK 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 251 bits (642), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 148/435 (34%), Positives = 241/435 (55%), Gaps = 16/435 (3%) Query: 13 LDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLAMASLTPASGWLADRFGTRRVYFLAIL 72 +D+T+++T+LP++A + +++ + SY +++A P SGW+ADRFG+ ++ AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 73 IFVLGSLLCANAHSLSQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQYISALAFISIAG 132 +F+LGS+LC + +L Q + AR +QG+GG+M++P+GRL +LRSV + AL++++I Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 133 QLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLP--AEEIVSPPRFD---- 186 GPV+GP GG+++ W WIF IN+P+ ++G++ RF+P E V+P Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 187 -MLGCALLSTCMVAFSLALDVPMEEHRAWWSAGLFGISVLTVWWYVVHARRDEQPLFRLG 245 LGC+LL + L V E AGL ++ YV HARR PL L Sbjct: 181 SALGCSLLMMGLATAGRHL-VSGEVALTCVLAGLVFLAA-----YVWHARRASAPLIDLA 234 Query: 246 LFNEPNFSVGLIGNLICRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGTIAKPW 305 L P G+ G R+G A+PFLLPL+LQ+ G++P H+G++ +A K Sbjct: 235 LLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTI 294 Query: 306 IAPLVRRYGYENFLLVNTVMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQFAAMNS 365 A +RRYG+ LLVN + + + A TP P + +V L + G S+QF ++N+ Sbjct: 295 AARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNA 354 Query: 366 VTLKGLNSKDAGSGNSLFSMVQMLALGLGVTIAG---SMLSGIDGVAWARDWSFTLTFVC 422 ++ ++ S+ S+ Q L++G+GV +AG M + I G A F F+ Sbjct: 355 ISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLV 414 Query: 423 VGLVTLLSALVFRRM 437 VG V + L+ R++ Sbjct: 415 VGAVAACALLMLRQL 429 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 239 bits (609), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 137/423 (32%), Positives = 235/423 (55%), Gaps = 6/423 (1%) Query: 13 LDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLAMASLTPASGWLADRFGTRRVYFLAIL 72 +D T++ T+LP++A L + + + + +Y A+ P SGW+AD+ G R+V+ A++ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 73 IFVLGSLLCANAHSLSQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQYISALAFISIAG 132 +F+ GS+LCA + SL + AR +QG+GG+M++P+GR+ + RSV ++ + A++++++ Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 133 QLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPAEEIVSPPRFDMLGCAL 192 LGPVVGP+ GG+++ W W+FLIN+P +G++ RF+ + +P D+ G L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 193 LSTCMVAFSLALDVPMEEH--RAWWSAGLFGISVLTVWWYVVHARRDEQPLFRLGLFNEP 250 S L L + H + I + + Y H+ + E PL L P Sbjct: 181 TSVGAAMIMLGLSL-FGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIP 239 Query: 251 NFSVGLIGNLICRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGTIAKPWIAPLV 310 ++G + RIG AVPFLLPL LQ LG + AG + +A + ++ Sbjct: 240 TLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVL 299 Query: 311 RRYGYENFLLVNTVMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQFAAMNSVTLKG 370 RRYG+ L+VN V G A+ ++ + + P + + + + GV ++QF +NS+ Sbjct: 300 RRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYAD 359 Query: 371 LNSKDAGSGNSLFSMVQMLALGLGVTIAGSML---SGIDGVAWARDWSFTLTFVCVGLVT 427 ++S+DAG SL S++Q L+LGLGVT+AG +L + + G A + F F+ +GL + Sbjct: 360 IDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFS 419 Query: 428 LLS 430 + S Sbjct: 420 VAS 422 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 227 bits (579), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 146/448 (32%), Positives = 241/448 (53%), Gaps = 18/448 (4%) Query: 2 WIVASGFFMQTLDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLAMASLTPASGWLADRF 61 +IVA+ FFM+ LDTTI+ TALP MA S G + + +Y L +A P SGW+ADRF Sbjct: 34 YIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRF 93 Query: 62 GTRRVYFLAILIFVLGSLLCANAHSLSQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQY 121 G+R V+ +AI IF L S+LC + ++ + ARV+QG+GG+M++P+GR+ V+RS + Sbjct: 94 GSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRL 153 Query: 122 ISALAFISIAGQLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPAEEIVS 181 + A+A I+ + PVVGP GG+++ WHWIFL+NVP G+ AV + E VS Sbjct: 154 MKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVS 213 Query: 182 PPRFDMLGCALLSTCMVAFSLALDVPME-EHRAWWSAGLFGISVLTVWWYVVHARRDEQP 240 D++G L + + ++ E + G + V+ + H +R E P Sbjct: 214 KRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHP 273 Query: 241 LFRLGLFNEPNFSVGLIGNLICRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGT 300 L P +SV ++ + RIG AVP+L+PL+ Q+ G + +GL+LL A+ Sbjct: 274 LLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNL 333 Query: 301 IAKPWIAPLVRRYGYENFLLVNTVMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQF 360 K + +++R+G+ + + N + G I++ L+TPA P+ + + + +G+ SMQF Sbjct: 334 GMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRSMQF 393 Query: 361 AAMNSVTLKGLNSKDAGSGNSLFSMVQMLALGLGVTIAGSML--------SGIDGVAWAR 412 + + ++ ++ S ++L+S+ Q + +G+G+ L ID A Sbjct: 394 STLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAID----AS 449 Query: 413 DWSFTL-----TFVCVGLVTLLSALVFR 435 FTL F G+VTL+S + +R Sbjct: 450 GAHFTLGDFQWAFAMAGVVTLISVIGYR 477 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 140 bits (353), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 124/427 (29%), Positives = 205/427 (48%), Gaps = 57/427 (13%) Query: 4 VASGFFMQTLDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLAMASLTPASGWLADRFGT 63 V+ FM LD++I N A+P+++ +LG V V+ S+ A A P +GWL R G Sbjct: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 Query: 64 RRVYFLAILIFVLGSLLCANAHSLSQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQYIS 123 R++ ++L+FVL S LC A +L LL ARV+QG+ ++P+ + +L + P ++ Sbjct: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 Query: 124 ALAFISIAGQLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIG---MFAVHRFLPAEEIV 180 ALA + + PV GP GGW++ + W WIF IN+PVG+I A++R E Sbjct: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYR--SRETPT 206 Query: 181 SPPRFDMLGCALLSTCMVAFSLALDVPMEEHRAWWSA----GLFGISVLTVWWYVVHARR 236 D +G ALL T + AF + LD + W+++ L I+++ ++VV Sbjct: 207 HKLPIDKIGLALLVTWVAAFQIMLD--KGKDLDWFASPTIVTLGIIALIAFAYFVVWELN 264 Query: 237 DEQPLFRLGLFNEPNFSVGLI----------GNLICRIGSSAVPFLLPLLLQVKLGFSPL 286 ++ P+ L LF NF+ G I GNL+ +LP LQ LG+ + Sbjct: 265 NDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLV----------ILPQWLQQYLGYRSI 314 Query: 287 HAGLVLLPAAVAGTIAKPWIAPLVRRYGYENFLLVNTVMVG-AAIVSFALITPAWPIW-- 343 +GL P + I + PL+ R+ LV+ V A+ + FA++ W Sbjct: 315 DSGLSTAPIGIFAVI----LMPLIGRF----LPLVDARKVATASFIGFAIV-----FWLR 361 Query: 344 ----IGIVQLAVF------GVCNSMQFAAMNSVTLKGLNSKDAGSGNSLFSMVQMLALGL 393 + + Q+ V G+ +M F + + L GL + + L S V++ + Sbjct: 362 SRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAV 421 Query: 394 GVTIAGS 400 G +I+ + Sbjct: 422 GTSISTT 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,371,026 Number of extensions: 58406 Number of successful extensions: 403 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 379 Number of HSP's successfully gapped: 18 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)