BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0028.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 358 e-100 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 176 2e-45 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 63 3e-11 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 62 6e-11 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 62 9e-11 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 358 bits (919), Expect = e-100, Method: Compositional matrix adjust. Identities = 196/307 (63%), Positives = 233/307 (75%), Gaps = 11/307 (3%) Query: 1 MIVIIGSINMDLVLRVPRMPLPGETITGGQFRTIPGGKGANQAVACARLSADDVKVAMVA 60 MIVIIGS+NMDLVLRVPRMPLPGET+ G +F TIPGGKGANQAVACARL+A +VAMVA Sbjct: 1 MIVIIGSVNMDLVLRVPRMPLPGETLAGDRFMTIPGGKGANQAVACARLAAPGTRVAMVA 60 Query: 61 CLGDDAFGAELRAALRKDGIDDSHVTTIPGTASGIASILVDDNGQNSIVLASGANAELSP 120 C+GDDAFG ++R ++ GIDD ++ + G A+GIASI+VD N QNSIV+A+GAN L Sbjct: 61 CVGDDAFGGQMRQSITACGIDDRYIDEVAGEATGIASIMVDANAQNSIVIAAGANGRLDV 120 Query: 121 AHIDAARDLIGQARIVVLQLESPLPTVIHAIKLAHALGKTVVLNPAPAQALPTELLGQID 180 I+ AR LI QA IV+LQLE P+ TVIH+I+LAHALGKTVVLNPAPAQALP LL +ID Sbjct: 121 ERIERARALIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPRALLQKID 180 Query: 181 YLVPNEIEAAMLAGMPAGDIRIENDSDEQIAAVIGKLRAAGSTNVLVTLGEKGVYAALES 240 YL+ NEIEAAMLA + E I + KL G+ NV+VTLGEKGVY + Sbjct: 181 YLILNEIEAAMLA----------EEQSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFAD 230 Query: 241 GSG-HFDAQAVKAVDTTAAGDTFIGGFVAALAEGRSEAEAIALGQRAAAISVARVGAQTS 299 G H A+ V+AVDTTAAGDTFIGGF+ A+A+GR + EAIA Q AAA+SV RVGAQTS Sbjct: 231 GQQRHLPARKVQAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTS 290 Query: 300 IPSRREV 306 IP+R EV Sbjct: 291 IPTRDEV 297 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 176 bits (446), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 125/306 (40%), Positives = 166/306 (54%), Gaps = 13/306 (4%) Query: 2 IVIIGSINMDLVLRVPRMPLPGETITGGQFRTIPGGKGANQAVACARLSADDVKVAMVAC 61 + ++G DL R P MP G+TI G F PGGKG+NQAVA AR+ A V ++ Sbjct: 10 VAVLGIYVADLAFRAPNMPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGA---PVRFISA 66 Query: 62 LGDDAFGAELRAALRKDGIDDSHVTTIPGTASGIASILVDD-NGQNSIVLASGANAELSP 120 +G DAFG + R++ I HV I G +G A I V+D +G N+I++ GA ++LS Sbjct: 67 IGKDAFGDFALSLWRRETIA-PHVRVIDGAPTGAAFIYVNDASGDNAIIVVPGAASQLSE 125 Query: 121 AHIDAARDLIGQARIVVLQLESPLPTVIHAIKLAHALGKTVVLNPAPAQALPTELLGQID 180 ++ R I A++ V QLE P + +++A A G T V NPAPA A P ++ G D Sbjct: 126 QDVERERQAIEAAKVFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALAFPAQVYGLCD 185 Query: 181 YLVPNEIEAAMLAGMPAGDIRIENDSDEQIAAVIGKLRAAGSTNVLVTLGEKGVYAALES 240 ++ PNE EAA+L G I I + D + AA + R G L+TLG +G S Sbjct: 186 FITPNEHEAALLTG-----IEIHHIDDARRAADVLLERGVGC--ALITLGAQGSLLHSRS 238 Query: 241 GSGHFDA-QAVKAVDTTAAGDTFIGGFVAALAEGRSEAEAIALGQRAAAISVARVGAQTS 299 S H A A V+T AGD F G F A LAEG S EA L AAISV R G S Sbjct: 239 QSLHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPS 298 Query: 300 IPSRRE 305 +PSR+E Sbjct: 299 MPSRQE 304 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 73/263 (27%), Positives = 110/263 (41%), Gaps = 26/263 (9%) Query: 58 MVACLGDDAFGAELRAALRKDGIDDSHVTTIPGTASGIASILVDDNGQNSIVL-----AS 112 ++ +GDD FG +L A +++ +D HV PG ++GI + D G + AS Sbjct: 49 YLSAVGDDHFGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAAS 108 Query: 113 GANAELSPAHIDAARDLIGQARIVVLQLESPLPTVIHAIKLAHALGKTVVLNP------- 165 A+ P AA + + I + ES + A+ A A G T L+ Sbjct: 109 AYQAKALPLEAIAAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLW 168 Query: 166 --APAQALPTELLGQIDYLVPNEIEAAMLAGMPAGDIRIENDSDEQIAAVIGKLRAAGST 223 A+A E D +P+ + ++L G+ D D A++ +L + G Sbjct: 169 PLERARARMEEAFRLCDICLPSWEDVSLLTGL--------EDRD----AIVDRLLSYGVK 216 Query: 224 NVLVTLGEKGVYAALESGSGHFDAQAVKAVDTTAAGDTFIGGFVAALAEGRSEAEAIALG 283 V LG++G Y A V ++D T AGD F G F+A L GR A Sbjct: 217 LVAFKLGKEGCYVATSDQRRMVAPYPVDSLDATGAGDCFGGAFMAELMAGRDAFAAARYA 276 Query: 284 QRAAAISVARVGAQTSIPSRREV 306 AAA+S GA IP R +V Sbjct: 277 NVAAALSTTGYGAVEPIPRRAQV 299 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 62.0 bits (149), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 75/324 (23%), Positives = 136/324 (41%), Gaps = 34/324 (10%) Query: 2 IVIIGSINMDLVLRVPRMPLPGETITGGQFRTIPGGKGANQAVACARLSADDVKVAMVAC 61 ++ IG + +DL + PL + F GG AN A CARL +K AM+A Sbjct: 23 LICIGRLAVDLYAQQVGAPLSDVS----SFAKYLGGSSANIAFGCARLG---LKSAMLAR 75 Query: 62 LGDDAFGAELRAALRKDGIDDSHVTTIPGTASGIASILVDDNGQNSIVLA--SGANAELS 119 +GD+A G L+A L +G D SHVT P + + + + D ++ + A+ L+ Sbjct: 76 IGDEANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSFPLIFYRDNCADMALA 135 Query: 120 PAHIDAARDLIGQAR-IVVLQLESPLPTVIHAIKLA------HALGKTVVLNPAPA-QAL 171 P ID A I ++ +++ P V + LA H + + ++ P L Sbjct: 136 PEDIDEA--FIASSKALLITGTHFSTPQVHRSSSLALQYARRHNVRTVLDIDYRPVLWGL 193 Query: 172 PTELLGQIDYLVPNEIEAAMLAGMPAGDIRIENDSDEQIA-------AVIGKLRAAGSTN 224 ++ G+ ++ + + + +P D+ + + + IA A + +RA + Sbjct: 194 TSKGDGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDIMAALRAVRALCAAT 253 Query: 225 VLVTLGEKG-------VYAALESGSGHFDAQAVKAVDTTAAGDTFIGGFVAALAEGRSEA 277 ++V G G + L+ G H+ V+ ++ AGD FI GF+ + Sbjct: 254 LVVKRGPLGCVVLHGAIPERLDDGF-HYRGVQVEVLNVLGAGDAFISGFLKGWLNEEDDE 312 Query: 278 EAIALGQRAAAISVARVGAQTSIP 301 A+ V+R ++P Sbjct: 313 HCCRYANACGALVVSRHACAPAMP 336 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 61.6 bits (148), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 75/291 (25%), Positives = 121/291 (41%), Gaps = 39/291 (13%) Query: 37 GKGANQAVACARLSADDVKVAMVACLGDDAFGAELRAALRKDGIDDSHVTT--------- 87 G N A+ ARL +KV + +G+DAFG +R + ++G++ S V T Sbjct: 37 GAETNVAIGLARLG---LKVGWASRVGNDAFGRFIRQRVAQEGVEVSRVITDMEFRTAIQ 93 Query: 88 IPGTASGIASILVDDNGQNSIVLASGANAELSPAHIDAARDLIGQARIVVLQLESPLPTV 147 + A G A ++ + S + + + AAR L A + L + Sbjct: 94 LKAKAVGGADPAIEYYRKGSAASHLSVD-DFDAGYFGAARHL--HATGIAPALSATTMAF 150 Query: 148 IH-AIKLAHALGKTVVLNP------APAQALPTELLGQI----DYLVPNEIEAAMLAGMP 196 H A+ GKT+ +P P+Q + + L + D+++P G+ Sbjct: 151 AHQAMDFMRGQGKTISFDPNLRPMLWPSQEVMAQQLNALAFKADWVLP---------GLS 201 Query: 197 AGDIRIENDSDEQIAAVIGKLRAAGSTNVLVTLGEKGVYAALESGSGHFDAQAVK-AVDT 255 G I +D +IA G G V++ LG +G Y G G VK VDT Sbjct: 202 EGKILTGHDDAREIA---GFYLERGVKLVVIKLGAEGAYWRNGQGEGRVAGVPVKEVVDT 258 Query: 256 TAAGDTFIGGFVAALAEGRSEAEAIALGQRAAAISVARVGAQTSIPSRREV 306 AGD F G ++ + EG +A+ G R A ++ VG +P+R E+ Sbjct: 259 VGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEGLPTRAEL 309 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 998,982 Number of extensions: 45006 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 6 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)