BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0021.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312720|ref|YP_003776812.1| enoyl-[acyl-carrier-protein] re... 494 e-141 gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carr... 200 1e-52 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 75 5e-15 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 64 1e-11 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 55 8e-09 >gi|300312720|ref|YP_003776812.1| enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 494 bits (1271), Expect = e-141, Method: Compositional matrix adjust. Identities = 243/260 (93%), Positives = 253/260 (97%) Query: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDTKLI 60 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFD+KL+ Sbjct: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLV 60 Query: 61 FDCDVGSDEQINALFADLGKSWDQLDGLVHAIGYAPADAIAGDFLEGFSREGFKIAHDIS 120 FDCDVGSDEQINALFADLGKSW QLDGLVHAIG+AP++AIAGDFLEGFSREGFKIAHDIS Sbjct: 61 FDCDVGSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 Query: 121 AYSFPAMAKAALPLLRQNSALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK 180 AYSFPAMAKAA+PLL N+ALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK Sbjct: 121 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK 180 Query: 181 GVRVNGVSAGPIKTLAASGIKGFGKILGFVSDHAPLRRNVTIEDVGNASAFLLSDLAGGI 240 GVRVNGVSAGPIKTLAASGIKGFGKILG V+ APLRRNVTIEDVGNASAFLLSDLA GI Sbjct: 181 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI 240 Query: 241 TGEITYVDGGFSRVVGGISE 260 TGEITYVDGGFSRVVGG++E Sbjct: 241 TGEITYVDGGFSRVVGGMAE 260 >gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 200 bits (508), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 104/251 (41%), Positives = 155/251 (61%), Gaps = 1/251 (0%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDTKLIFDC 63 L+GK+ LI G+ SIA+G A+A + GA+LA T++ E + + A++ ++ Sbjct: 5 LEGKRGLIVGIADKHSIAWGCARALRAAGADLAVTWLNESARRYVEPLADQVQASIMCGL 64 Query: 64 DVGSDEQINALFADLGKSWDQLDGLVHAIGYAPADAIAGDFLEGFSREGFKIAHDISAYS 123 DV ++ +F + W LD ++H+I +APA + G + S +GF +A D+S +S Sbjct: 65 DVTHPGEMEQVFDLIRTHWGSLDFVLHSIAFAPAPDLHGR-VTNCSSQGFSLAMDVSCHS 123 Query: 124 FPAMAKAALPLLRQNSALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKGVR 183 F MA+ A PL++ +LLT++Y+G+E VV NY MG KA+LE++VRYLA LGP G+R Sbjct: 124 FIRMARLAEPLMKNGGSLLTMSYIGAEEVVVNYGVMGPVKAALESAVRYLAMELGPAGIR 183 Query: 184 VNGVSAGPIKTLAASGIKGFGKILGFVSDHAPLRRNVTIEDVGNASAFLLSDLAGGITGE 243 VN VS GP+ T AASG+ F + L + +PLRR I+DVG+ +FL+SD A ITG Sbjct: 184 VNAVSPGPLATRAASGLNCFEQFLTDAARRSPLRRLANIDDVGDMCSFLVSDAASVITGN 243 Query: 244 ITYVDGGFSRV 254 YVD G+ V Sbjct: 244 TLYVDAGYHIV 254 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 75.5 bits (184), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 79/260 (30%), Positives = 121/260 (46%), Gaps = 31/260 (11%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRIT-KFAEEFDTK-LIF 61 L GK LITG + I +A A REGA LA Y+ E R T K + D L+ Sbjct: 123 LAGKVALITG--GDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLL 180 Query: 62 DCDVGSDEQINALFADLGKSWDQLDGLVH-AIGYAPADAIAGDFLEGFSREGFKIAHDIS 120 DVGS E + + ++ +LD LV+ A PA +I E S+ + + Sbjct: 181 PGDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSI-----EEISQAQLEHTFRTN 235 Query: 121 AYSFPAMAKAALPLLRQNSALLTLT----YLGSERVVPNYNTMGLAKASLEASVRYLAES 176 +S M K A+P L + + ++ T Y GS+ +V +Y K ++ A R L++ Sbjct: 236 IFSMFYMVKEAMPFLTRGARIINTTSVTAYRGSKHLV-DY---AATKGAIVAFTRSLSQQ 291 Query: 177 LGPKGVRVNGVSAGPIKT------LAASGIKGFGKILGFVSDHAPLRRNVTIEDVGNASA 230 L G+ VN V+ GPI T +A + FGK + PL R ++V A Sbjct: 292 LASAGIHVNAVAPGPIWTPLIPASFSAEEVASFGK-------NVPLGRPGQPDEVAPAYV 344 Query: 231 FLLSDLAGGITGEITYVDGG 250 FL ++ A +TG++ + +GG Sbjct: 345 FLATEGASYMTGQVLHPNGG 364 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 70/262 (26%), Positives = 114/262 (43%), Gaps = 28/262 (10%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDTKLIFDC 63 L GK L+TG S I AQ +GA++ T + D T + D Sbjct: 5 LNGKIALVTGGTSG--IGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRADA 62 Query: 64 DVGSDEQINALFADLGKSWDQLDGLVHAIGYAPADAIAGDFLE--GFSREGFKIAHDISA 121 V S +++ ++A + KS +LD L A+A GD + + E F + Sbjct: 63 SVLS--ELDTVYAQIAKSAGRLDILF-------ANAGGGDMMPLGAITEEHFDRIFGTNV 113 Query: 122 YSFPAMAKAALPLLRQNSALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKG 181 + ALPLL ++++ + S N++ +KA++ R A L + Sbjct: 114 RGVLFTVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRA 173 Query: 182 VRVNGVSAGPIKTLAASGIKGFGKIL---------GFVSDHAPLRRNVTIEDVGNASAFL 232 +RVN VS GPI+T G G+++ +++ PL R +++G A AFL Sbjct: 174 IRVNVVSPGPIRT------PGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFL 227 Query: 233 LSDLAGGITGEITYVDGGFSRV 254 SD A + G +VDGG ++V Sbjct: 228 ASDAASFVNGIELFVDGGMAQV 249 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 54.7 bits (130), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 44/141 (31%), Positives = 74/141 (52%), Gaps = 11/141 (7%) Query: 118 DISAYSFPAMAKAALPLLR--QNSALLTLTYLGSER---VV--PNYNTMGLAKASLEASV 170 D+S M++A LP +R QN +++ ++ + ++R ++ P+Y+ AKA + Sbjct: 110 DVSLRGTLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSA---AKAGVLGLA 166 Query: 171 RYLAESLGPKGVRVNGVSAGPIKTLAASGIKGFGKILGFVSDHAPLRRNVTIEDVGNASA 230 R +A GP+G+RVN ++ G I T G K + +++ PL R +D+G A Sbjct: 167 RAMAREFGPEGIRVNSITPGLIGTDIIKG-KLTEEKKAEIAETIPLARLGRADDIGGACV 225 Query: 231 FLLSDLAGGITGEITYVDGGF 251 FL SDL+ TG V+GG Sbjct: 226 FLGSDLSQYCTGITLDVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 805,022 Number of extensions: 34715 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 14 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)