BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0014.p (517 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Her... 952 0.0 gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Her... 602 e-173 gi|300312143|ref|YP_003776235.1| homocitrate synthase [Herbaspir... 79 1e-15 >gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 567 Score = 952 bits (2461), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/509 (88%), Positives = 479/509 (94%) Query: 1 MLNNPAAKYRAFPTIPLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFFEM 60 ML+NPAAKYR FP I L DRTWP++ I+ PPIWMSTDLRDGNQALIEPM+ ERKLRFFE+ Sbjct: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVINTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 Query: 61 LLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLAGA 120 LL+ GLKEIEVGFPSASQTDFDFVRKLI ENRIPDDVTIIVLTQSREELIRRTI+SL GA Sbjct: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTIESLEGA 121 Query: 121 KQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPESFS 180 K+AIVHLYNSVAPAFRKIVFNMSR+EIKNIAVTGT+LVKELTDARP T+WR+EYSPESFS Sbjct: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFS 181 Query: 181 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSSSI 240 TTELDFSKEICDAVCETWGA+PERKVILNLPSTVEC+TPNVYADQIEWMCRNLK R+S+I Sbjct: 182 TTELDFSKEICDAVCETWGASPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRASTI 241 Query: 241 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 300 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD Sbjct: 242 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 Query: 301 FSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVPYL 360 FSDID VRQ VEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQ DAIWEVPYL Sbjct: 302 FSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYL 361 Query: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASGKE 420 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADA+GKE Sbjct: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKE 421 Query: 421 IVATDIYTIFKTEYLDRSTPYVYRAHRMSEDTSHEESIKIEVDIERNGEKTTVRGTGNGP 480 I A+DI+ IF+ EYL+R PYVYRAHRMSED+S ESI IEVDI RNG+ TVRG+GNGP Sbjct: 422 IAASDIHAIFQREYLERVEPYVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNGP 481 Query: 481 IDAFVNALGLDIKLMDFHEHAIGAAPTRK 509 IDAFV+ALGLDIKLMDFHEHAIGA K Sbjct: 482 IDAFVHALGLDIKLMDFHEHAIGAGADAK 510 >gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 575 Score = 602 bits (1552), Expect = e-173, Method: Compositional matrix adjust. Identities = 288/521 (55%), Positives = 376/521 (72%), Gaps = 20/521 (3%) Query: 1 MLNNPAAKYRAFPTIPLSDRTWPNQFISKPPIWMSTDLRDGNQALIEPMSPERKLRFFEM 60 ML +P+ KYRA + L DR WP++ + + P W+STDLRDGNQAL EPM+ ERKLR F Sbjct: 1 MLTDPSQKYRAIAPVDLPDRRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 Query: 61 LLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPDDVTIIVLTQSREELIRRTIDSLAGA 120 L++IG KEIEVGFP+AS+ DF+ VR LI+E+ IPDDVT +V+TQ R +LI T+ S+AGA Sbjct: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 Query: 121 KQAIVHLYNSVAPAFRKIVFNMSRDEIKNIAVTGTQLVKELTDARPETQWRYEYSPESFS 180 ++ IVH YN++APA+R+IVF MS +I + L + LT PET+W +YSPE+F Sbjct: 121 RRVIVHFYNAIAPAWREIVFGMSVPQIITLVEDHIALFRRLTAVHPETEWILQYSPETFC 180 Query: 181 TTELDFSKEICDAVCETWGATPERKVILNLPSTVECSTPNVYADQIEWMCRNLKHRSSSI 240 EL+ S E+C+A W A P R++I+NLP+TVE STPNV+ADQIEWM R L+ R I Sbjct: 181 MAELEVSLEVCNAAIRAWDAGPRRRMIINLPTTVEVSTPNVFADQIEWMDRRLERREHLI 240 Query: 241 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 300 +SVHPHNDRGTAVA AE A++AGA RVEGCLFGNGER+GNVD+VTLALNLYTQG+ PGLD Sbjct: 241 LSVHPHNDRGTAVACAEQAMLAGAQRVEGCLFGNGERSGNVDVVTLALNLYTQGIAPGLD 300 Query: 301 FSDIDTVRQCVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQADAIWEVPYL 360 FSDI + + EEC +P+HPRHPYVGDLVFTAFSGSHQDAI KGFA Q+ DA W VPYL Sbjct: 301 FSDIAALARVAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAAQRPDAPWRVPYL 360 Query: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADASGKE 420 PIDP DLGR+YD+++RVNSQSGKGG+A+LL++++ + +PRR+Q+EFS +Q ADAS E Sbjct: 361 PIDPTDLGRTYDSIVRVNSQSGKGGIAFLLQRDHHITMPRRMQVEFSAIVQALADASETE 420 Query: 421 IVATDIYTIFKTEYL----------DRST--------PYVYRAHRMSEDTSHEESIKIEV 462 + + ++ +F+ YL DR++ ++YR+H + E + Sbjct: 421 LTSEHLWDVFERTYLAPVQASPDFSDRTSLAPALEQRRFIYRSHCLHPHPDGERIVLELA 480 Query: 463 DIERNGEKTTVRGTGNGPIDAFVNALGLDIKLMDFHEHAIG 503 D E G TV G+GNGPI A V ALGL +++ + E ++G Sbjct: 481 DTE--GNVRTVEGSGNGPIAATVAALGLALRIDSYEERSLG 519 >gi|300312143|ref|YP_003776235.1| homocitrate synthase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 78.6 bits (192), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 92/349 (26%), Positives = 140/349 (40%), Gaps = 39/349 (11%) Query: 36 TDLRDGNQALIEPMSPERKLRFFEMLLQIGLKEIEVGFPSASQTDFDFVRKLIEENRIPD 95 T LRDG Q + E K+ L +IG+ E+E+G P Q + + ++ + +P Sbjct: 7 TTLRDGEQTAGVAFTAEEKIDIARALDEIGVPEMEIGIPIMGQAEIEVIQAIAA---LP- 62 Query: 96 DVTIIVLTQSREELIRRTIDS-LAGAKQAIVHLYNSVAPAFR-KIVFNMSRDEIKNIAVT 153 +S+ + R ++ LA A + + N P I + RD +A Sbjct: 63 -------LRSKTMVWGRMCEADLAAAARCNADIVNLSMPVSDIHIQHKLQRDRDWVLAQI 115 Query: 154 GTQLVKELTDARPETQWRYEYSPESFSTTELDFSKEICDAVCETWGATPERKVILNLPST 213 L K L + E S +LDF V ET A R+ T Sbjct: 116 RHFLPKAL-----DLGMEVCLGGEDSSRADLDFMLR----VIETAQAAGARR--FRFADT 164 Query: 214 VECSTPNVYADQIEWMCRNLKHRSSSIISVHPHNDRGTAVASAELAVMAGADRVEGCLFG 273 + P + I R L+ + +H HND G A A+ A+ AGA V + G Sbjct: 165 LGLLDPFATYEVI----RQLRQEVDIELEMHAHNDLGLATANTLAAINAGASHVNTTVNG 220 Query: 274 NGERTGNVDLVTLALNL-YTQGVNPGLDFSDIDTVRQCVEECNQIPVHPRHPYVGDLVFT 332 GER GN L + + L + G+ +D + + + V + + V P VG+ VFT Sbjct: 221 LGERAGNAPLEEVVMGLRHLHGIETQVDTRSLPAISRLVAKASGKAVAPNKSIVGEAVFT 280 Query: 333 AFSGSHQDAIKKGFAKQQADAIWEVPYLPIDPADLGRSYDAVIRVNSQS 381 SG H D + K A Y PA+LGRS+ V+ +S S Sbjct: 281 HESGIHVDGLLKNPAN----------YEHFSPAELGRSHHIVLGKHSGS 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,614,737 Number of extensions: 66985 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 3 Length of query: 517 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 428 Effective length of database: 1,198,455 Effective search space: 512938740 Effective search space used: 512938740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)