BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0005.p (343 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 628 0.0 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 354 6e-99 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 290 2e-79 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 280 9e-77 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 249 2e-67 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 628 bits (1620), Expect = 0.0, Method: Compositional matrix adjust. Identities = 301/343 (87%), Positives = 323/343 (94%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRA+ADAG Sbjct: 135 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYADAG 194 Query: 61 IPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHA 120 +P GV+NLVYGVP+EISEYLIPHP+IKKISFTGSTPVGKQLAALAG HMKRATMELGGHA Sbjct: 195 VPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATMELGGHA 254 Query: 121 PAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFEAFVDKFITAASLLKVGD 180 PA+VFDDAD+D AAK LA AKFRNAGQVCVSPTRFLVQK VF AFVDKF+T A LKVG+ Sbjct: 255 PAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYAEQLKVGN 314 Query: 181 GTVAGTTMGPVANKRRIPALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVLTNVPLSA 240 G AG TMGPVANKRRIPALEALI DAV QGAQLR GGKR+G+KGYFF+PTVLTN+PL A Sbjct: 315 GLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTVLTNLPLQA 374 Query: 241 RAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRVESGMITI 300 RAMNEEPFGPLALINSF+TLDEVI EANRLP+GLAAYA+AKSA TKHAL++RVESGM+TI Sbjct: 375 RAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARVESGMLTI 434 Query: 301 NHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQLDV 343 NHLGLALPE+PFGGIKDSGYGSEGGTEAIEAY+NTK V+QLDV Sbjct: 435 NHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQLDV 477 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 354 bits (908), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 180/341 (52%), Positives = 248/341 (72%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 +V++EPVG AA +PWNFP Q ++K++AA+AAGC++++K P E+PA+ + + F DAG Sbjct: 135 LVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVLMAQLFHDAG 194 Query: 61 IPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHA 120 +P GV N+V GV SEIS++LI P+++ ISFTGS PVG +LAA A HMKR +MELGGHA Sbjct: 195 VPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKRMSMELGGHA 254 Query: 121 PAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFEAFVDKFITAASLLKVGD 180 P +VFDDA+++ AA+ LA K RNAGQVCVSP+RF VQK ++ FV F A +KVG Sbjct: 255 PVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAEALLQIKVGP 314 Query: 181 GTVAGTTMGPVANKRRIPALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVLTNVPLSA 240 G G MGP+ + RR A++ LI DA+ QG++L GG R+G +G+F+ PT++T VP SA Sbjct: 315 GHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHFYAPTLITGVPPSA 374 Query: 241 RAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRVESGMITI 300 R M EEPFGP+A + F +E IA AN LP GLA+Y + +S T H +S +++GM+ I Sbjct: 375 RVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRVSRDLQAGMVNI 434 Query: 301 NHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQL 341 NH G+A PE+PFGGI +SG+GSEGG E E+++ TK +SQ+ Sbjct: 435 NHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQI 475 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 290 bits (741), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 158/347 (45%), Positives = 222/347 (63%), Gaps = 7/347 (2%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 M +KEPVG VAA TPWNFP + RK++ ALAAGC+++ K E+TP + L+R +AG Sbjct: 144 MALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALVRLAQEAG 203 Query: 61 IPDGVVNLVYGVPS---EISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELG 117 +P GV+N+V E+ + + ++KISFTGST VGK LA + +K+ ++ELG Sbjct: 204 VPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLKKLSLELG 263 Query: 118 GHAPAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFEAFVDKFITAASLLK 177 G+AP IVFDDAD+DAA + AKFRN GQ CVSP R VQ+ V++AFVDK + LK Sbjct: 264 GNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKLGARVAALK 323 Query: 178 VGDGTVAGTTMGPVANKRRIPALEALISDAVEQGAQLRFGGKRVGDKGY----FFEPTVL 233 VG T + +GP+ N R I ++ + DA+ +GA++ GGKR+ G+ ++ PTVL Sbjct: 324 VGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDNYYAPTVL 383 Query: 234 TNVPLSARAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRV 293 +V + + EE FGP+A I F T DEVIA AN PFGLAAY Y+ + H ++ + Sbjct: 384 ADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRIHRVTDAL 443 Query: 294 ESGMITINHLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQ 340 ESG++ +N LA PFGG+K+SGYG EG ++ Y++TK+V Q Sbjct: 444 ESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVCQ 490 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 280 bits (717), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 154/340 (45%), Positives = 209/340 (61%), Gaps = 2/340 (0%) Query: 1 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAG 60 +VIKE +G AA TPWNFP + RK ALAAGC +++K E TP S L AG Sbjct: 142 VVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALAVLAERAG 201 Query: 61 IPDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHA 120 IP GV ++V G P I + +PI++KISFTGST VGK L + + +K+ ++ELGG+A Sbjct: 202 IPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNA 261 Query: 121 PAIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFEAFVDKFITAASLLKVGD 180 P IVFDDAD+DAA + +K+RNAGQ CV R VQ V++AF K + A KVG Sbjct: 262 PFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQ 321 Query: 181 GTVAGTTMGPVANKRRIPALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVLTNVPLSA 240 GT G T GP+ N++ + +E ++DAV +GA++ GGKR FFEPTVL +V + Sbjct: 322 GTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTPAM 381 Query: 241 RAMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRVESGMITI 300 + EE FGP+A + F T +EV+A AN FGLA+Y Y++ ++ +ESGM+ I Sbjct: 382 QVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGI 441 Query: 301 NHLGLALPEV-PFGGIKDSGYGSEGGTEAIEAYVNTKFVS 339 N GL EV PFGG+K SG G EG I+ Y+ K++ Sbjct: 442 N-TGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 249 bits (637), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 137/340 (40%), Positives = 191/340 (56%) Query: 2 VIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAFADAGI 61 ++EP+G V A PWN PL K++A LAAGC++++K EETP S L R +AG Sbjct: 142 TVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETPQSALHLARLAQEAGF 201 Query: 62 PDGVVNLVYGVPSEISEYLIPHPIIKKISFTGSTPVGKQLAALAGTHMKRATMELGGHAP 121 PDGV+N+V G + + L HP + KISFTGS G+ + +AG KR T+ELGG +P Sbjct: 202 PDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGGKSP 261 Query: 122 AIVFDDADIDAAAKQLAMAKFRNAGQVCVSPTRFLVQKNVFEAFVDKFITAASLLKVGDG 181 IVFDDA + A + F N GQVC + +R LVQ+++ + F AA+ + VGD Sbjct: 262 QIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDP 321 Query: 182 TVAGTTMGPVANKRRIPALEALISDAVEQGAQLRFGGKRVGDKGYFFEPTVLTNVPLSAR 241 + G MGPVA K + + I +++GA L GG DKG+F +PT+ + Sbjct: 322 SQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIFADANNGMS 381 Query: 242 AMNEEPFGPLALINSFDTLDEVIAEANRLPFGLAAYAYAKSAQTKHALSSRVESGMITIN 301 EE FGP+ I SFDT +E IA AN +GLAA + + H +++ V+ G + +N Sbjct: 382 IAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVN 441 Query: 302 HLGLALPEVPFGGIKDSGYGSEGGTEAIEAYVNTKFVSQL 341 +P+GGIKDSG G EGG AY K V+ L Sbjct: 442 CWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTVL 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,029,711 Number of extensions: 43328 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 20 Length of query: 343 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 257 Effective length of database: 1,212,660 Effective search space: 311653620 Effective search space used: 311653620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)