BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0030.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309949|ref|YP_003774041.1| heat shock protein 24 (HSP-70 c... 244 3e-66 >gi|300309949|ref|YP_003774041.1| heat shock protein 24 (HSP-70 cofactor)protein [Herbaspirillum seropedicae SmR1] Length = 189 Score = 244 bits (623), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 117/135 (86%), Positives = 129/135 (95%) Query: 45 QDAFLRAKAEAENIRRRAQDDISRAHKFAIEGFAESLLSVKDSLEMALKIETPSVESLKE 104 QDAFLRA+AE ENIRRRAQ+DI++AHKFAIEGFAESLL+VKDSLEMALKIE S+ESLKE Sbjct: 55 QDAFLRARAEGENIRRRAQEDIAKAHKFAIEGFAESLLAVKDSLEMALKIENASLESLKE 114 Query: 105 GVDMTLKQLSAAFEKNKLLEISPEAGEKLDPMKHQAISAVPAEQEANTVVTVLQKGYTIS 164 GVDMTLKQLS+AFEKNKL E++P+AG+KLDPMKHQA+SAVPAEQEANTVV VLQKGY IS Sbjct: 115 GVDMTLKQLSSAFEKNKLQEVNPQAGDKLDPMKHQAVSAVPAEQEANTVVAVLQKGYMIS 174 Query: 165 ERLLRPALVTVAQGK 179 ERLLRPALVTVAQGK Sbjct: 175 ERLLRPALVTVAQGK 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.308 0.123 0.310 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 437,917 Number of extensions: 14254 Number of successful extensions: 52 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 52 Number of HSP's successfully gapped: 1 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 127 (53.5 bits)