BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0021.p (170 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309942|ref|YP_003774034.1| small protein A [Herbaspirillum... 199 8e-53 >gi|300309942|ref|YP_003774034.1| small protein A [Herbaspirillum seropedicae SmR1] Length = 181 Score = 199 bits (507), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 91/130 (70%), Positives = 112/130 (86%), Gaps = 1/130 (0%) Query: 38 GVQVSKKS-SLFGFLKPYRIDIQQGNFVSKEMVAQLKPGMTRDQVRFVLGTPLLADVFHS 96 GV+VS KS SLFGFL+PYRIDIQQGNFVS+EMVAQL+PG+TRDQVRFVLGTPLL D+FH+ Sbjct: 44 GVKVSGKSNSLFGFLRPYRIDIQQGNFVSREMVAQLRPGLTRDQVRFVLGTPLLTDMFHT 103 Query: 97 DRWDYEFRLAKGNGELMSSRVSVFFKDNLMERYEGGSDLPTEQEYLSLIAGSKKGAMPEL 156 +RWDY+FRLAKGNGE+M+SRV+VFFKD+L++ +GG DLPTE EYL+LI +K G+ +L Sbjct: 104 NRWDYDFRLAKGNGEIMTSRVTVFFKDDLLDHVQGGQDLPTESEYLTLITDAKVGSKAKL 163 Query: 157 SDTPTNTAPP 166 +D A P Sbjct: 164 TDDDVKPASP 173 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 490,168 Number of extensions: 18097 Number of successful extensions: 45 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 45 Number of HSP's successfully gapped: 1 Length of query: 170 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 91 Effective length of database: 1,245,805 Effective search space: 113368255 Effective search space used: 113368255 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 127 (53.5 bits)