BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0013.p (163 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309935|ref|YP_003774027.1| spermidine/putrescine ABC trans... 285 2e-78 >gi|300309935|ref|YP_003774027.1| spermidine/putrescine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 285 bits (728), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 143/163 (87%), Positives = 150/163 (92%) Query: 1 MMPVAVPGLATSLALIMAYGQYRAFRGSLLFILVGHVLFTLPFMVRPVLAVMQSSQLAVL 60 MMPVAVPGLAT+LALIMAYGQYR FR SL FILVGHV+FTLPFMVRPVLAVMQSSQLA L Sbjct: 105 MMPVAVPGLATALALIMAYGQYREFRASLWFILVGHVIFTLPFMVRPVLAVMQSSQLAQL 164 Query: 61 EEAAASLGAGMRQRFFGVVIPNAASGILAGALMVVTLSIGEFNITWMLHTPLTMTLPVGL 120 EEAAASLGAG +RFFG+VIPNAASGILAGALMVVTLSIGEFNITWMLHTPLT TLPVGL Sbjct: 165 EEAAASLGAGRMRRFFGIVIPNAASGILAGALMVVTLSIGEFNITWMLHTPLTQTLPVGL 224 Query: 121 ADAYASMRLEVGSAYTLIFLLMIVPLLVAMQWATQTTRTRVRL 163 ADAYASMRLEVGSAYTLIF MI+PLL+ MQWATQ TR +V L Sbjct: 225 ADAYASMRLEVGSAYTLIFFFMIIPLLIGMQWATQATRKKVSL 267 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.139 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 497,831 Number of extensions: 20306 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 3 Length of query: 163 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 85 Effective length of database: 1,250,540 Effective search space: 106295900 Effective search space used: 106295900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 127 (53.5 bits)