BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0006.p (881 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbasp... 1620 0.0 gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 82 2e-16 gi|300312383|ref|YP_003776475.1| methionyl-tRNA synthetase [Herb... 60 5e-10 gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 57 7e-09 >gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 882 Score = 1620 bits (4195), Expect = 0.0, Method: Compositional matrix adjust. Identities = 793/882 (89%), Positives = 839/882 (95%), Gaps = 1/882 (0%) Query: 1 MQDKYSPAEVEQSAQQHWIATDAYKTVEHAKDKHGRDKKKFYACSMLPYPSGKLHMGHVR 60 MQDKYSPAEVEQSAQQHW A DAYKTVE+A+DK GRDKKKFYACSMLPYPSGKLHMGHVR Sbjct: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKQGRDKKKFYACSMLPYPSGKLHMGHVR 60 Query: 61 NYTINDVMYRYLRMSGYNVLMPMGWDAFGMPAENAAMANGVPPAQWTYSNIEYMKKQMAS 120 NYTINDVMYRYLRM+GYNVLMPMGWDAFGMPAENAAMANGVPPAQWTYSNI+YMK QM S Sbjct: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPAENAAMANGVPPAQWTYSNIDYMKSQMQS 120 Query: 121 MGLAIDWSREMTACSPEYYKWNQWMFLKMLEKGIIYKKTGTVNWDPIDQTVLANEQVIDG 180 MGLAIDWSREMTAC PEYYKWNQWMFLKMLEKGIIYKKTGTVNWDPIDQTVLANEQVIDG Sbjct: 121 MGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNWDPIDQTVLANEQVIDG 180 Query: 181 KGWRSGAVIEKREIPMYYAKITDYAEELLEHVETKLPGWPERVRLMQANWIGKSTGVRFA 240 +GWRSGAVIEKREIPMYYAKITDYA+ELLEHVETKLPGWPERVRLMQANWIGKSTGVRFA Sbjct: 181 RGWRSGAVIEKREIPMYYAKITDYADELLEHVETKLPGWPERVRLMQANWIGKSTGVRFA 240 Query: 241 FPHDIKD-DGKLINDGKLWVFTTRADTVMGVTFCAVAPEHALASFAAKNNPALQDFIAEC 299 FPH IK DG+LINDGKL+VFTTRADT+MGVTFCAVAPEH LA+ AA+N+PALQ FIAEC Sbjct: 241 FPHQIKGADGQLINDGKLFVFTTRADTIMGVTFCAVAPEHPLATLAAENDPALQAFIAEC 300 Query: 300 KKGSVIEADMATMEKKGMPTGLFVTHPLTGAQVEVWVGNYVLITYGDGAVMGVPAHDERD 359 K+GSVIEADMATMEKKGMPTGLFVTHPLTG QVEVWVGNYVLITYGDGAVMGVPAHDERD Sbjct: 301 KQGSVIEADMATMEKKGMPTGLFVTHPLTGDQVEVWVGNYVLITYGDGAVMGVPAHDERD 360 Query: 360 FAFAKKYNLAIRQVVAVEGKTYSTDAWEEWYGDKEGGKTVDSGKYDGLGYQAAVDAVAAD 419 FAFA+KYNL I+QV+ VEGKTYST+AWEEWYGDKE GKT+ SGKYDGLGYQAAVDAVAAD Sbjct: 361 FAFARKYNLPIKQVIGVEGKTYSTEAWEEWYGDKEAGKTIHSGKYDGLGYQAAVDAVAAD 420 Query: 420 LAAKGLGEKKVTFRLRDWGISRQRYWGTPIPIIHCADCGDVPVPEKDLPVVLPEDCVPDG 479 LAA+GLGEKKVTFRLRDWGISRQRYWGTPIPIIHC DCGDVPVPEKDLPVVLPEDCVPDG Sbjct: 421 LAAQGLGEKKVTFRLRDWGISRQRYWGTPIPIIHCPDCGDVPVPEKDLPVVLPEDCVPDG 480 Query: 480 SGNPLNKHEKFLHVDCPSCGKPARRETDTMDTFVDSSWYYMRYTSPQSNDAMVDSRNDYW 539 SGNPLNKHE+FL VDCP CGKPARRETDTMDTFVDSSWYYMRYTSP SN+AMVDSRNDYW Sbjct: 481 SGNPLNKHEQFLKVDCPCCGKPARRETDTMDTFVDSSWYYMRYTSPGSNEAMVDSRNDYW 540 Query: 540 MPMDQYIGGIEHAVLHLLYARFWTKVMRDFGLVKFDEPFTNLLTQGMVLNETYYREDAAG 599 MPMDQYIGGIEHAVLHLLYARFWTKVMRDFGLVKFDEPFTNLLTQGMVLNETYYREDAAG Sbjct: 541 MPMDQYIGGIEHAVLHLLYARFWTKVMRDFGLVKFDEPFTNLLTQGMVLNETYYREDAAG 600 Query: 600 KKTWINPADVELTHDDKGRPVTAILKEDGQPVIIGGTEKMSKSKNNGIDPQAQIDQYGAD 659 KKTW NPADV+L+ DDKGRPV+A+LKEDGQPV+IGGTEKMSKSKNNGIDPQAQIDQYGAD Sbjct: 601 KKTWYNPADVDLSFDDKGRPVSAVLKEDGQPVMIGGTEKMSKSKNNGIDPQAQIDQYGAD 660 Query: 660 TARLFTMFASPPEQTLEWSGTGVEGANRFLRRVWAFAHNNAARIEGAAAVDASQLTDALK 719 TARLFTMFASPPEQTLEWSG GVEGANRFLRRVWAF +NNAARI A AVDA+QL D+LK Sbjct: 661 TARLFTMFASPPEQTLEWSGAGVEGANRFLRRVWAFGYNNAARISAAGAVDAAQLDDSLK 720 Query: 720 TLRREVHKILQQADHDLKRIQYNTVVSACMKMLNTLEAAKLEDSPVANAVLAECTSIFLR 779 TLRREVHK+LQQAD+D KRIQYNTVVSACMKMLNTLEAAK +AV+AEC SIFLR Sbjct: 721 TLRREVHKVLQQADNDFKRIQYNTVVSACMKMLNTLEAAKPAAGAAGDAVIAECFSIFLR 780 Query: 780 ILNPITPHITHALWQELGFAKQQGDILDADWPQVDAAALEQSEIEMMIQVNGKLRGSITV 839 +LNP+ PHITH LWQELG+AK QGDILDADWPQVDAAALEQSEI++++QVNGKLRG +TV Sbjct: 781 VLNPVAPHITHVLWQELGYAKAQGDILDADWPQVDAAALEQSEIKLVVQVNGKLRGEVTV 840 Query: 840 AKDADKASIEAAALANESVQKFLTGAPKKIIVVPGKLINIVA 881 +KDADKA+IEAAALANE V+KFLTGAPKK+IVVPGKL+NIVA Sbjct: 841 SKDADKAAIEAAALANEDVRKFLTGAPKKVIVVPGKLVNIVA 882 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 82.0 bits (201), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 58/203 (28%), Positives = 88/203 (43%), Gaps = 26/203 (12%) Query: 1 MQDKYSPAEVEQSAQQHWIATDAYKTVEHAKDKHGRDKKKFYACSMLPYPSGKLHMGHVR 60 + + PAE+E+ ++ W + A +K F P +G LHMGH Sbjct: 38 LAKSFEPAEIEKFWREEWEKRGYFAATTDA------EKPSFSIQLPPPNVTGTLHMGHAF 91 Query: 61 NYTINDVMYRYLRMSGYNVLMPMGWDAFGMPA----ENAAMANGVPPA------------ 104 N TI D + RY RM GYN G D G+ E A V Sbjct: 92 NQTIMDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVW 151 Query: 105 QWTYSNIEYMKKQMASMGLAIDWSREMTACSPEYYKWNQWMFLKMLEKGIIYKKTGTVNW 164 +W + + QM MG + DW+RE P+ K +F+++ E+G+IY+ VNW Sbjct: 152 EWKEKSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNW 211 Query: 165 DPIDQTVLANEQVI----DGKGW 183 DP+ T +++ +V+ DG W Sbjct: 212 DPVLGTAVSDLEVVSEEEDGSMW 234 >gi|300312383|ref|YP_003776475.1| methionyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 690 Score = 60.5 bits (145), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 40/140 (28%), Positives = 66/140 (47%), Gaps = 3/140 (2%) Query: 39 KKFYACSMLPYPSGKLHMGHVRNYTINDVMYRYLRMSGYNVLMPMGWDAFGMPAENAAMA 98 ++ + + LPY +G H+GH+ Y D+ R+ RM G V DA G P AA Sbjct: 7 RQLFVTTALPYANGAFHIGHIMEYIQADIWVRFQRMQGNEVHFVGADDAHGAPIMIAAEK 66 Query: 99 NGVPPAQWTYSNIEYMKKQMASMGLAIDWSREMTACSPEYYKWNQWMFLKML-EKGIIYK 157 G+ P Q+ +NI +KQ G I + + SPE ++ +Q ++ + E +I Sbjct: 67 AGITPQQFV-ANIAAGRKQYLD-GFHIAFDNWSSTDSPENHELSQEIYRALRDEAKLIST 124 Query: 158 KTGTVNWDPIDQTVLANEQV 177 KT +DP+ L + + Sbjct: 125 KTIDQFYDPVKNMFLPDRYI 144 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 57.0 bits (136), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 41/160 (25%), Positives = 65/160 (40%), Gaps = 10/160 (6%) Query: 15 QQHWIATDAYKTVEHAKDKHGRDKKKFYACSMLPYPSGKLHMGHVRNYTINDVMYRYLRM 74 + W+ K V K + KF PY +G++H+GH N + D++ + M Sbjct: 42 EPQWVKQWQEKKVYERIRKASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKARNM 101 Query: 75 SGYNVLMPMGWDAFGMPAE---NAAMANGVPPAQ-----WTYSN--IEYMKKQMASMGLA 124 +G++ GWD GMP E +P A+ Y+N I KK +G+ Sbjct: 102 AGFDAQYVPGWDCHGMPIEIQIEKKYGKNLPVAEVQAKARAYANEQIALQKKDFIRLGVL 161 Query: 125 IDWSREMTACSPEYYKWNQWMFLKMLEKGIIYKKTGTVNW 164 +W + K+LEKG +Y+ VNW Sbjct: 162 GEWDNPYKTMNFSNEADELRALGKILEKGYVYRGLKPVNW 201 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,894,478 Number of extensions: 126882 Number of successful extensions: 368 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 366 Number of HSP's successfully gapped: 4 Length of query: 881 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 787 Effective length of database: 1,174,780 Effective search space: 924551860 Effective search space used: 924551860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)