BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0016.0001.p
         (201 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate...   337   5e-94
gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate...    88   6e-19
gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate...    86   3e-18

>gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 201

 Score =  337 bits (863), Expect = 5e-94,   Method: Compositional matrix adjust.
 Identities = 163/199 (81%), Positives = 178/199 (89%)

Query: 1   MGKVCEYFFAPHSPFAYLGHARFVALARQYDVQVVLKPFDLSKIFGQSGGLPLAKRAPQR 60
           M KVCEYFFAPHSPFAYLGH RFVALA +Y VQ++ KPFDLSKIFG SGGLPLAKRAPQR
Sbjct: 1   MSKVCEYFFAPHSPFAYLGHDRFVALADKYQVQILPKPFDLSKIFGVSGGLPLAKRAPQR 60

Query: 61  QAYRLKELERWSKFLGLPMNLQPTYFPVQSDVAARLIIATQLAHGTSAALDLTGAVMRAI 120
           QAYRLKEL RWS+FLG PM LQP +FPV SD AARLIIATQLAHGT+AAL L GAVMRA+
Sbjct: 61  QAYRLKELARWSEFLGKPMILQPAHFPVNSDNAARLIIATQLAHGTAAALRLAGAVMRAV 120

Query: 121 WEEEKNIADADTLLAIANGLGHDGASLLKSAETASVQAEFDRFSEEATTANVFGAPWFIV 180
           WEE+KNIAD DTL+AIAN   HDGA+LLKSAETASVQAEFDRF++EA  A+VFGAPWFI 
Sbjct: 121 WEEQKNIADPDTLVAIANVQEHDGAALLKSAETASVQAEFDRFTDEANAASVFGAPWFIY 180

Query: 181 DGEGYWGQDRLDFVERAFA 199
           +GEGYWGQDRLDF+ERAFA
Sbjct: 181 EGEGYWGQDRLDFLERAFA 199


>gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 200

 Score = 87.8 bits (216), Expect = 6e-19,   Method: Compositional matrix adjust.
 Identities = 59/195 (30%), Positives = 96/195 (49%), Gaps = 3/195 (1%)

Query: 1   MGKVCEYFFAPHSPFAYLGHARFVALARQYDVQVVLKPFDLSKIFGQSGGLPLAKRAPQR 60
           M    +++F   SP+ Y        LA Q+   V   P  L  IF   G   L    P +
Sbjct: 1   MSNPIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVN-IPVK 59

Query: 61  QAYRLKELERWSKFLGLPMNLQPTYFPVQSDVAARLIIATQLAHGTSAALDLTGAVMRAI 120
             Y   ++ER ++F  +     P++FP+ + VAAR  +  Q     + A++L   +  A 
Sbjct: 60  GEYSRHDMERTARFHNI-FYKAPSHFPIGTQVAARATLWVQQTQ-PAKAVELIKTLYSAY 117

Query: 121 WEEEKNIADADTLLAIANGLGHDGASLLKSAETASVQAEFDRFSEEATTANVFGAPWFIV 180
           + E+ +I+  D +L IA  LG D  +L  + E+  ++ +  + +E A  A VFG+P+ IV
Sbjct: 118 FTEDIDISVVDNVLRIAADLGIDRNALQAALESPELKDQLRQSTEAAGQAGVFGSPFMIV 177

Query: 181 DGEGYWGQDRLDFVE 195
           DGE +WG DR   +E
Sbjct: 178 DGEQFWGFDRFPQLE 192


>gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 206

 Score = 85.5 bits (210), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 60/192 (31%), Positives = 99/192 (51%), Gaps = 7/192 (3%)

Query: 3   KVCEYFFAPHSPFAYLGHARFVALARQYDVQVVLKPFDLSKIFGQSGGLPLAKRAPQRQ- 61
           K+ EY+F   S ++YL   R    A+Q  V+VV +PF L  +F Q  G   +    Q++ 
Sbjct: 6   KLIEYWFDFGSNYSYLSTMRIEQAAQQAGVRVVWRPFLLGPVF-QELGWSTSPFVLQKEK 64

Query: 62  -AYRLKELERWSKFLGLPMNLQPTYFPVQSDVAARLIIATQLAHGTSAALDLTGAVMRAI 120
            AY  K++ R  +  GLP  + P+ FP  + +A R+ ++ + A   S+   L   VM   
Sbjct: 65  GAYTWKDMTRQCRKYGLPWTM-PSRFPRAAVLATRVALSFEDALWMSSFCRL---VMHLN 120

Query: 121 WEEEKNIADADTLLAIANGLGHDGASLLKSAETASVQAEFDRFSEEATTANVFGAPWFIV 180
           + E+++I   +T+  +   LG   A ++  A++   +A     + +A T  +FGAP F V
Sbjct: 121 FAEDRDIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTLGIFGAPTFFV 180

Query: 181 DGEGYWGQDRLD 192
             E +WG DRLD
Sbjct: 181 GDEMFWGNDRLD 192


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 643,693
Number of extensions: 25824
Number of successful extensions: 78
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 3
Length of query: 201
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 120
Effective length of database: 1,236,335
Effective search space: 148360200
Effective search space used: 148360200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 128 (53.9 bits)