BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0193.p (624 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 424 e-119 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 414 e-117 gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 78 2e-15 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 424 bits (1089), Expect = e-119, Method: Compositional matrix adjust. Identities = 249/552 (45%), Positives = 338/552 (61%), Gaps = 25/552 (4%) Query: 33 EFFSLKKLAESYPA-IHSWPRAKLILLENLLRHENG---TETSRTLIESYLSGDSDDTPI 88 +F+SL L +S I P + I+LE++LR+ +G TE + ++ + + I Sbjct: 21 KFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVTEEHVKQLANWGAKAARVDEI 80 Query: 89 PFFPARVLMQDYAGISALVDIAGLRNVLEEHGVAADSISPVIPVDLVVDHSLQVNSHGSS 148 PF ARV++QD+ G+ L D+A +RNV + G +I P++PVDLVVDHS+Q++ Sbjct: 81 PFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEPLVPVDLVVDHSVQIDHFREK 140 Query: 149 GAPAENLMDEYRLNKSRYEFLRWAEQSISNLRVVPPGRGIVHQVNVEVLSTGTRVKHNEI 208 A N+ E+ N RY+F++W Q+ VVPPG GIVHQVN+E L+ G K Sbjct: 141 KALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHNKTGVY 200 Query: 209 FFDTLVGTDSHTPMVNGLGILGWGVGGIEALAAMLGQPLWLSTPRVTGVRLIGEVPAGVY 268 + DTLVGTDSHT M+NG+G++GWGVGGIEA A MLGQP++ TP V GV L G + GV Sbjct: 201 YPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGALREGVT 260 Query: 269 AADIALNLTAFLRRIGVVGNFLEFFGPGSASLSVFDKSTIANMTPEYGATCSLFPVTQST 328 A D+ L +T LR+ VVG F+EFFG G+ SLS+ D++TIANM PEYGAT FPV +T Sbjct: 261 ATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATIANMAPEYGATMGFFPVDDAT 320 Query: 329 LDYLGQAKRSDLPGESADALRAYLDAQGMLDDSDQEPL-FDRVVEFDLGAVQPSFSGPSR 387 ++Y R+ DA AY AQ + + + + V DLG V PS +GP R Sbjct: 321 IEYFKGTGRTK---AEIDAFEAYFKAQELYGVPKAGQIDYTQEVSLDLGTVAPSLAGPKR 377 Query: 388 PDQVHGLSELTAAF---LSSPGAKTD-NAPAE-----------AHIRNGAIAIAAITSCT 432 P + + + F + P A+ N AE + NG + IAAITSCT Sbjct: 378 PQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADGVDLHNGDVLIAAITSCT 437 Query: 433 NTANPKSMLAAGLVARKAIALGLRVPSHVKCSFTPGSIAVDRYLDQAGLLEPLNQLGFNL 492 NT+NP +LAAGL+A+KA+ GL+V H+K S PGS V +YL+ AGLL L +LGF + Sbjct: 438 NTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAAGLLPYLEKLGFGV 497 Query: 493 AGYGCATCVGNSGELKPEVQQAIDTGGSELVLASVLSGNRNFESRIHPSIGANFLMSPAL 552 YGC TC+GN+G+L P + +AI +++V A+VLSGNRNFE+RIHP+I ANFL SP L Sbjct: 498 TAYGCTTCIGNAGDLTPAMNEAIVK--NDVVAAAVLSGNRNFEARIHPNIRANFLASPPL 555 Query: 553 VVVLAIAGXRQR 564 VV AIAG R Sbjct: 556 VVAYAIAGNVTR 567 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 414 bits (1065), Expect = e-117, Method: Compositional matrix adjust. Identities = 237/544 (43%), Positives = 326/544 (59%), Gaps = 22/544 (4%) Query: 23 RTALPGFPALEFFSLKKLAESYP--AIHSWPRAKLILLENLLRH---ENGTETSRTLIES 77 R LPG L++F + E+ A + P +L ENL+R T++ R LIE Sbjct: 6 RKQLPG-TRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDSLRQLIER 64 Query: 78 YLSGDSDDTPIPFFPARVLMQDYAGISALVDIAGLRNVLEEHGVAADSISPVIPVDLVVD 137 D P+FPARV+ D G +ALVD+AGLR+ + + G ++PV+PV L+VD Sbjct: 65 KRELD-----FPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQLIVD 119 Query: 138 HSLQVNSHGSS-GAPAENLMDEYRLNKSRYEFLRWAEQSISNLRVVPPGRGIVHQVNVEV 196 HSL V G A +N E R N+ R+ F+ W + + N+ V+PPG GI+HQ+N+E Sbjct: 120 HSLAVECGGDDPQAFVKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQINLEK 179 Query: 197 LSTGTRVKHNEIFFDTLVGTDSHTPMVNGLGILGWGVGGIEALAAMLGQPLWLSTPRVTG 256 +S K F DTLVGTDSHTP V+ LG++ GVGG+EA MLG+ W+ P + G Sbjct: 180 MSPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIG 239 Query: 257 VRLIGEVPAGVYAADIALNLTAFLRRIGVVGNFLEFFGPGSASLSVFDKSTIANMTPEYG 316 V L G G+ A DI L+LT FLR+ VVG +LEF+G G+ASL++ D++TI+NM PEYG Sbjct: 240 VELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMAPEYG 299 Query: 317 ATCSLFPVTQSTLDYLGQAKRSDLPGESADALRAYLDAQGMLDDSDQEPLFDRVVEFDLG 376 AT ++F + Q T+DYL R D E + Y G+ D+ + ++RV+ FDL Sbjct: 300 ATAAMFSIDQQTIDYLKLTGRED---EQVKLVETYAKEAGLWSDTLAKVEYERVLRFDLS 356 Query: 377 AVQPSFSGPSRPDQVHGLSELTAAFLSSPGAKTDNAPAEAHIRNGAIAIAAITSCTNTAN 436 V + +GPS P + +SEL A ++ K +N P + + +GA+ IAAITSCTNT+N Sbjct: 357 TVVRTLAGPSNPHKRLPVSELAAQGIA---GKVENEPGK--MPDGAVIIAAITSCTNTSN 411 Query: 437 PKSMLAAGLVARKAIALGLRVPSHVKCSFTPGSIAVDRYLDQAGLLEPLNQLGFNLAGYG 496 P++++AAGL+AR A LGL VK S PGS AV+ YL++AGL L +LGF + + Sbjct: 412 PRNVIAAGLLARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFA 471 Query: 497 CATCVGNSGELKPEVQQAIDTGGSELVLASVLSGNRNFESRIHPSIGANFLMSPALVVVL 556 C TC G SG L P++QQ I +L +VLSGNRNF+ RIHP FL SP LVV Sbjct: 472 CTTCNGMSGALDPKIQQEIID--RDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAY 529 Query: 557 AIAG 560 AIAG Sbjct: 530 AIAG 533 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 77.8 bits (190), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 95/357 (26%), Positives = 140/357 (39%), Gaps = 49/357 (13%) Query: 212 TLVGTDSHTPMVNGLGILGWGVGGIEALAAMLGQPLWLSTPRVTGVRLIGEVPAGVYAAD 271 T+V DSHT L G+G E + Q L + V++ G +PAGV A D Sbjct: 123 TVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTAKD 182 Query: 272 IALNLTAFLRRIGVVGNFLEFFGPGSASLSVFDKSTIANMTPEYGATCSLFPVTQSTLDY 331 I L + + G G +EF G SLS+ + T+ NM E GA + +TL+Y Sbjct: 183 IVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEY 242 Query: 332 LGQAKRSDLPGESADALRAYLDAQGMLDDSDQEPLFDRVVEFDLGAVQPSFSGPSRPDQV 391 L + + G + +Y + SD FD VV + ++P + + P+ V Sbjct: 243 L-KGRPFSPSGPHWERAVSYWRSL----HSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMV 297 Query: 392 HGLSELTAAFLSSPGAKTDNAPAEAHIRNGAIAIAA----------------ITSCTNTA 435 A P + P + A+A A I SCTN+ Sbjct: 298 ------VAVDARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSR 351 Query: 436 NPKSMLAAGLVARKAIALGLRVPSHVKCSF-TPGSIAVDRYLDQAGLLEPLNQLGFNLAG 494 AA +V K A S+VK + PGS V ++ GL + GF Sbjct: 352 IEDLREAAKVVRGKFRA------SNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWRE 405 Query: 495 YGCATCVG-NSGELKPEVQQAIDTGGSELVLASVLSGNRNFESRIHPSIGANFLMSP 550 GC+ C+ N+ L+P + A + NRNFE R + G L+SP Sbjct: 406 PGCSMCLAMNADRLEPGERCAS-------------TSNRNFEGR-QGAGGRTHLVSP 448 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,959,457 Number of extensions: 83952 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 254 Number of HSP's successfully gapped: 3 Length of query: 624 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 533 Effective length of database: 1,188,985 Effective search space: 633729005 Effective search space used: 633729005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)