BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0188.p (412 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313460|ref|YP_003777552.1| branched-chain amino acid ABC t... 354 8e-99 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 89 5e-19 gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 85 1e-17 gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 77 4e-15 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 61 2e-10 >gi|300313460|ref|YP_003777552.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 430 Score = 354 bits (908), Expect = 8e-99, Method: Compositional matrix adjust. Identities = 176/416 (42%), Positives = 270/416 (64%), Gaps = 15/416 (3%) Query: 7 LSLAVAALFSLPAYAQVKVAQLDGISGPFANVGEQQRKTLLAMFDDVNAKGGL---NGQK 63 L LA++ + A +++ +DG+SGPFAN GE + + + VNA+GG+ +G+ Sbjct: 18 LPLALSPARAAAPLAPIRIGMIDGLSGPFANAGEAVVRNISYAIERVNARGGVTLPDGKH 77 Query: 64 L-ELVTIDGKGSPQDSLNAFKQAVDQGIRIVTQGNSSAVAAALSDAVAKYNQRNPASPVL 122 L +L T D K +DSL F+Q D+ I + +GNSSAVAAAL DAV K+NQR P VL Sbjct: 78 LLQLSTFDNKLGVEDSLVQFRQLTDERIPFLIEGNSSAVAAALIDAVNKHNQREPDRRVL 137 Query: 123 YLNHSGIDNDLTNSKCNFWHFRFDPNVDMKVEAITSELVKNKNIKSVYLINQNYALGQQA 182 +LN+S +D LTN KC+FWHFRFD + DM+++A+T + + + VYLI Q+Y+ G+Q Sbjct: 138 FLNYSAVDPTLTNEKCSFWHFRFDASADMRMQALTEAIKADTATRKVYLIGQDYSFGRQV 197 Query: 183 SAAAKRIIPSRRADIKIVGDDLHPFGQVKDFSSYAAKIKASGADAILTGNWGNDLSLLVK 242 + AA+ + ++R DI IVGD+LHP G++KDF+ Y AK++++GADA++TGNWGNDL+LLVK Sbjct: 198 AKAAREQLAAKRPDIAIVGDELHPIGKIKDFAPYIAKMRSAGADAVITGNWGNDLTLLVK 257 Query: 243 ALKDANVSVAIYTIYGSGFGAPTAIKENGVDHLKVMSVWNPNIKND---KSEEFANEFKK 299 A +DA YT Y + GAP AI E GV ++ ++ W+PN + S+ + F++ Sbjct: 258 AARDAGFKAKFYTFYANSLGAPAAIGEAGVGVVRAVAEWHPNAGGNPGSPSDAYYQAFRQ 317 Query: 300 KYGI---ELYFHQIRTMIQMLAAAANKSKSNDPKTIAYALEGMKFQADTGDAEMRKSDHQ 356 +Y + + +++ MI MLA A K+ S DPK +AYALEG ++Q+ A MR DHQ Sbjct: 318 RYPQPKDDYLYLRMQVMIDMLAKAIEKAGSADPKAVAYALEGAQYQSAFHSATMRAQDHQ 377 Query: 357 ILLPMFLSTMKKTASNGGPADVKYDSEGTGVVGFQVDSKIEASVSTVATTCQMVRP 412 ++ P++L M++ S G +++D+EG+G GF+ + I A + + ++C+M RP Sbjct: 378 LIQPLYLMQMQRADSGG----IRFDNEGSG-YGFRTERFIPAEQTALPSSCRMQRP 428 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 89.4 bits (220), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 85/338 (25%), Positives = 149/338 (44%), Gaps = 19/338 (5%) Query: 31 ISGPFANVGEQQRKTLLAMFDDVNAKGGLNGQKLELVTIDGKGSPQDSLNAFKQAVDQGI 90 +SGPFA G+ L M + G + G+ V +D +G P ++ ++++++G Sbjct: 39 MSGPFAANGKFAD---LGMKLALQKYGKVLGKSTSYVNLDTEGKPATAVRKMQESIEKGT 95 Query: 91 RIVTQGNSSAVAAALSDAVAKYNQRNPASPVLYLNHSGIDNDLTNSKCNFWHFRFD-PNV 149 R G S A A+ K + +++ +G D ++T S CN FR+ P Sbjct: 96 RFFAGGILSGEALAMGKEADK-------AGAVFITTAGAD-EITGSDCNRATFRWSVPTF 147 Query: 150 DMKVEAITSELVKNKNIKSVYLINQNYALGQQASAAAKRIIPSRRADIKIVGDDLHPFGQ 209 + + L K N K Y I Y G+ +AAK I + I+ VG+ H + Sbjct: 148 GAIEQTVRPLLEKMPNAKRWYTITPQYVFGEGLLSAAKNIF--KEKGIEHVGNSYHSLTE 205 Query: 210 VKDFSSYAAKIKASGADAILTGNWGNDLSLLVKALKDANV--SVAIYTIYGSGFGAPTAI 267 K+FS Y A+ D +L N+G+ S ++ + + I + SG + Sbjct: 206 -KEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETL 264 Query: 268 KENGVDHLKVMSVWNPNIKNDKSEEFANEFKKKYGIELYFHQIRTMI--QMLAAAANKSK 325 + D + + + I ++EF I+ + + I +++ A K+ Sbjct: 265 GADICDGVYFGAQYWHAIDAPFNKEFVKLVNDNLKIDPNYSLAGSYICTKIILDAIIKAN 324 Query: 326 SNDPKTIAYALEGMKFQADTGDAEMRKSDHQILLPMFL 363 S DPK + A+EGMK++ TG E+RK DHQ++ +L Sbjct: 325 STDPKAVIAAMEGMKYEGLTGSEEIRKGDHQVIKNYYL 362 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 83/352 (23%), Positives = 148/352 (42%), Gaps = 16/352 (4%) Query: 35 FANVGEQQRKTLLAMFDDVNAKGGLNGQKLELVTIDGKG-SPQDSLNAFKQAVDQGIRIV 93 + G+ R L + +NA GG+ G+ L+LV + G + Q + NA + ++ Sbjct: 49 LSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDADQAARNARALISQDHVEVL 108 Query: 94 TQGNSSAVAAALSDAVAKYNQRNPASPVLYLNHSGIDNDLTNSKCNFWHFRFDPNVDMKV 153 T S VA A+S A+ VL + + + + +T K N + FR P+ M+ Sbjct: 109 TGSMLSDVALAVSREAAR------GRTVLVVGDA-LSDAITLDKGNRYTFRVRPSTYMQA 161 Query: 154 EAITSELVKNKNIKSVYLINQNYALGQQASAAAKRIIPSRRADIKIVGDDLHPFGQVKDF 213 A+ +E + ++ NY GQ A A+ K ++ R D++ V D G++K Sbjct: 162 -AMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSDQWPALGKIKA- 219 Query: 214 SSYAAKIKASGADAILTGNWGNDLSLLVKALKDANVSVAIYTIYGSGFGAPT---AIKEN 270 A I+ S D I +G DL V+A + + + + GAP+ + E Sbjct: 220 DDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGAPSLDPSQSEA 279 Query: 271 GVDHLKVMSVWNPNIKNDKSEEFANEFKKKYGIELYFHQIRTMIQMLAAAAN--KSKSND 328 D L + W+ + + + F + K++ + M+A AA KS+ D Sbjct: 280 IRDWLAIGYPWD-QVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKKSEGGD 338 Query: 329 PKTIAYALEGMKFQADTGDAEMRKSDHQILLPMFLSTMKKTASNGGPADVKY 380 + + A+ G++F + G R D Q L F+ + + D +Y Sbjct: 339 GEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWRY 390 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 76.6 bits (187), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 77/335 (22%), Positives = 141/335 (42%), Gaps = 26/335 (7%) Query: 40 EQQRKTLLAMFDDVNAKGGLNGQKLELVTIDGKGSPQDSLNAFKQAV-DQGIRIVTQGNS 98 E R+ D++NA GG+ G+KLE++ D G+P DS+ ++ + + + ++ G Sbjct: 48 EPYRRGWEMALDEINASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFL 107 Query: 99 SAVAAALSDAVAKYNQRNPASPVLYLNHSGIDNDLTNSKCNFWHFRFDPNVDMKVEAITS 158 S AL+D ++ V +L + + + N + +R P+ M V ++ Sbjct: 108 SNTGLALTDYAKQHR-------VFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVK 160 Query: 159 ELVKNKNIKSVYLINQNYALGQQASAAAKRIIPSRRADIKIVGDDLHPFGQVKDFSSYAA 218 E VK K K ++ NY GQ A A+ K ++ + D++ V + P G++ D + Sbjct: 161 EAVKLKK-KRWAIVYPNYEYGQSAVASFKTLMKQAQPDVEFVAEQAPPLGKI-DAGAVTQ 218 Query: 219 KIKASGADAILTGNWGNDLSLLV-----KALKDANVSVAIYTIYGSGFGAPT---AIKEN 270 + + DAI + DL V + L + V++ T G P +K Sbjct: 219 ALADAKPDAIFNALFAADLGKFVREGNTRGLFEQVDVVSLLT------GEPEYLDPLKGE 272 Query: 271 GVDHLKVMSVWNPNIKNDKSEEFANEFKKKYGIELYFHQI--RTMIQMLAAAANKSKSND 328 + V I + ++F ++KK+ + + + +AA K+ S D Sbjct: 273 APQNWIVTGYPWYAIHTPEHKKFVEAYRKKFNDYPRNGSLVGYSALMSIAAGLRKAGSTD 332 Query: 329 PKTIAYALEGMKFQADTGDAEMRKSDHQILLPMFL 363 +A A G+K + R DHQ L F+ Sbjct: 333 ADRLAAAFSGLKVETPVASIVYRPQDHQSTLGAFV 367 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 71/341 (20%), Positives = 139/341 (40%), Gaps = 31/341 (9%) Query: 23 VKVAQLDGISGPFANVGEQQRKTLLAMFDDVNAKGGLNGQKLELVTIDGKGSPQDSLNAF 82 VK+A ++G A G+ + L + +NA GG NG K E V +D P+ ++ Sbjct: 29 VKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAVAVA 88 Query: 83 KQAVDQGIRIVTQGNSSAVAAALSDAVAKYNQRNPASPVLYLNHSGIDNDLTNSKCNFWH 142 + V QGI+ V S SD Y V+ + S LT +K + + Sbjct: 89 NKIVSQGIKYVIGHVCSGSTIPASDI---YENEG----VVMITPSATAPQLTEAKPHKFI 141 Query: 143 FRFDPNVDMKVEAITSELVKNKNIKSVYLINQNYALGQQASAAAKRIIPSRRADIKI--- 199 FR D + A +++ K V +++ + GQ +++ K + + + + + Sbjct: 142 FRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEG 201 Query: 200 --VGDDLHPFGQVKDFSSYAAKIKASGADAILTGNWGNDLSLLVKALKDANVSVAIYTIY 257 GD D+S+ K+K+ G D + G + ++ L+++ ++ + Sbjct: 202 INAGDS--------DYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPE 253 Query: 258 GSGFGAPTAIKENGVDHLKVM--SVWNPNIKNDKSEEFANEFKK----KYGIELYFHQIR 311 G G TAI + + V + + + N + K+ + + Y Sbjct: 254 GVGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDPNGPFQMPAY----- 308 Query: 312 TMIQMLAAAANKSKSNDPKTIAYALEGMKFQADTGDAEMRK 352 + ++++A A +KS DP+ +A + F+ G+ E K Sbjct: 309 SGVKIIADAIAGAKSTDPEKVAAYIHANSFKTPIGNVEYDK 349 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,152,454 Number of extensions: 45573 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 13 Length of query: 412 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 324 Effective length of database: 1,203,190 Effective search space: 389833560 Effective search space used: 389833560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)