BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0185.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 364 e-102 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 347 1e-96 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 333 1e-92 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 317 1e-87 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 303 2e-83 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 364 bits (935), Expect = e-102, Method: Compositional matrix adjust. Identities = 209/478 (43%), Positives = 294/478 (61%), Gaps = 10/478 (2%) Query: 2 LIGGVWQDSASGEWIDSIEPFSGDVIAQVPRGNEADAAQAVDAAYKAYRSPAWSELTATQ 61 I G +++ G+ ++ + P + ++ A +G AD A+AV AA AWS+L+ Q Sbjct: 8 FIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSGLQ 67 Query: 62 RGALLRKLGDLIERDAVRLADIERSCNGKNITEVSGQ-VRNVAQWFYYYAGLADKIHGSV 120 RG LL KL L+ERD LAD++ G++ E V N AG A+++ G Sbjct: 68 RGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEGRT 127 Query: 121 VP----INKPGVFNYVRYEPLGVVVAITPWNSPLALTAWKMAPALACGNTIVVKPSEFTS 176 +P + KP +Y EP+GVV AI PWN+PL +T+WK+A LA G T+V+KP+E T Sbjct: 128 IPTAGYMGKP-TLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETP 186 Query: 177 PPLLELAALASEAGFPPGVINVVSGYGNEIGAALINHPRVAKIAFTGGDIGGQKVAEAAA 236 L LA LA EAGFP GVINVV+GYGN +G AL HP VAKI+FTG G+ + A Sbjct: 187 QSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAG 246 Query: 237 KNFKKITLELGGKSANIVFADADFEQAINGAIAGVFGAAGQTCMAGSRLLVERSIHDKFV 296 + FK++TLELGGKS IVF DA FE A+ G G+F GQ C AGSR+LV+RS+ +F Sbjct: 247 EQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFA 306 Query: 297 ARLIEVAGAARIGNPADSETQVGPIATRSQYEKILGYIEIAKQDGATCVLGGNALSGAQY 356 L E A +G+P+ ++GP+A ++Q+E++ YI+ +GAT + GG ++ Sbjct: 307 KALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV----SRP 362 Query: 357 GQGQFVAPTIFTNVRNDMRIAREEVFGPILSVIPFDSVEEALEIANDTDFGLAAAVWTTD 416 +G FV PTIF + N M IAREE+FGP+ ++I FD+ EEA+ +AND+ +GLAA VWTT+ Sbjct: 363 DKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTN 422 Query: 417 LHKAIYCSDRIEAGTVWVNNYRATSFTTPFGGYKRSGVGREGGLEGAQEYMQAKSVWI 474 L +A + ++ G V VN + P+GG K SG+GREGGL GA Y + K+V + Sbjct: 423 LARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 480 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 347 bits (889), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 185/432 (42%), Positives = 256/432 (59%), Gaps = 7/432 (1%) Query: 54 WSELTATQRGALLRKLGDLIERDAVRLADIERSCNGKNITEVSGQVRNVAQWFYYYAGLA 113 W L+ RG L +G +I +A LA +E +GK I + +++ VA+ F YYAG A Sbjct: 75 WWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWA 134 Query: 114 DKIHGSVVPINKPGVFNYVRYEPLGVVVAITPWNSPLALTAWKMAPALACGNTIVVKPSE 173 DK +G V+P+ + NY R EP G V+ +TPWN+P W++ PALA GN +++KPSE Sbjct: 135 DKFYGEVIPVPSSHL-NYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSE 193 Query: 174 FTSPPLLELAALASEAGFPPGVINVVSGYGNEIGAALINHPRVAKIAFTGGDIGGQKVAE 233 T L +A L +AG P G++NV++G G + + V K+ F G G +A+ Sbjct: 194 LTPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAK 253 Query: 234 AAAKNFKKITLELGGKSANIVFADADFEQAINGAIAGVFGAAGQTCMAGSRLLVERSIHD 293 AAA LELGGKSANI+F DAD A GA A +F AGQ+C+AGSRLLV+R ++D Sbjct: 254 AAAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYD 313 Query: 294 KFVARLIEVAGAARIGNPADSETQVGPIATRSQYEKILGYIEIAKQDGATCVLGGNALSG 353 +FV + A R+G P D T+VGPI R QYE I + + GAT G Sbjct: 314 RFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHT---- 369 Query: 354 AQYG-QGQFVAPTIFTNVRNDMRIAREEVFGPILSVIPFDSVEEALEIANDTDFGLAAAV 412 +YG +G FV PT+ + N M +AR E+FGP+ IPF+ EEA+ IAND++FGLA AV Sbjct: 370 -RYGEEGYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAV 428 Query: 413 WTTDLHKAIYCSDRIEAGTVWVNNYRATSFTTPFGGYKRSGVGREGGLEGAQEYMQAKSV 472 WT D+ +A + + AGT WVN+Y+ + +PFGG+ RSG GR G+E +Y Q KSV Sbjct: 429 WTRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSV 488 Query: 473 WITTQPNKQNAF 484 W+ T + F Sbjct: 489 WVETSASPATPF 500 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 333 bits (855), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 193/490 (39%), Positives = 274/490 (55%), Gaps = 12/490 (2%) Query: 2 LIGGVWQDSASGEWIDSIEPFSGDVIAQVPRGNEADAAQAVDAAYKAYRSPAWSELTATQ 61 IGG + GE+ ++I P G +V R + D A+DAA+ A +S W + + T+ Sbjct: 22 FIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKS--WGKTSPTE 79 Query: 62 RGALLRKLGDLIERDAVRLADIERSCNGKNITE-VSGQVRNVAQWFYYYAGLADKIHGSV 120 R +L K+ D +E + LA E NGK I E ++ + F Y+A GS+ Sbjct: 80 RANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSI 139 Query: 121 VPINKPGVFNYVRYEPLGVVVAITPWNSPLALTAWKMAPALACGNTIVVKPSEFTSPPLL 180 PI+ + Y +EPLGVV I PWN P+ + WK+APALA GN +V+KP+E T ++ Sbjct: 140 CPIDN-DTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIM 198 Query: 181 ELAALASEAGFPPGVINVVSGYGNEIGAALINHPRVAKIAFTGGDIGGQKVAEAAAKNFK 240 L L ++ PPGV+N+V G+G E G L ++ R+AKIAFTG G+ + + A++N Sbjct: 199 VLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLI 257 Query: 241 KITLELGGKSANIVFADA--DFEQAINGAIAGVFGAA---GQTCMAGSRLLVERSIHDKF 295 +TLELGGKS NI FAD + + A+ G A G+ C SR+LV+ SI+++F Sbjct: 258 PVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYERF 317 Query: 296 VARLIEVAGAARIGNPADSETQVGPIATRSQYEKILGYIEIAKQDGATCVLGGNALS-GA 354 + R ++ A + GNP D T +G A++ Q EKIL YI+I KQ+GA + GG G Sbjct: 318 IERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGG 377 Query: 355 QYGQGQFVAPTIFTNVRNDMRIAREEVFGPILSVIPFDSVEEALEIANDTDFGLAAAVWT 414 G +V PT+F N MRI +EE+FGP++SV F EEAL IANDT +GL A +WT Sbjct: 378 DLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWT 436 Query: 415 TDLHKAIYCSDRIEAGTVWVNNYRATSFTTPFGGYKRSGVGREGGLEGAQEYMQAKSVWI 474 D +A I+AG VW N Y FGGYK+SG+GRE Y Q K++ + Sbjct: 437 RDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLV 496 Query: 475 TTQPNKQNAF 484 + P F Sbjct: 497 SYSPKALGFF 506 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 317 bits (813), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 185/475 (38%), Positives = 265/475 (55%), Gaps = 7/475 (1%) Query: 2 LIGGVWQDSASGEWIDSIEPFSGDVIAQVPRGNEADAAQAVDAAYKAYRSPAWSELTATQ 61 I W SGE I ++P G + RGN D QAV AA AY AW L+A + Sbjct: 5 FIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEG-AWGRLSAAE 63 Query: 62 RGALLRKLGDLIERDAVRLADIE-RSCNGKNITEVSGQVRNVAQWFYYYAGLADKIHGSV 120 R LL KL ++ LA +E R C GK + + + ++F +YAG ADK+HG Sbjct: 64 RSRLLMKLAYKLQDHQEELAQLEGRDC-GKPMKQARADAAAIVRYFEFYAGAADKLHGDT 122 Query: 121 VPINKPGVFNYVRYEPLGVVVAITPWNSPLALTAWKMAPALACGNTIVVKPSEFTSPPLL 180 +P + G EP GV I PWN P+ + + ALA GNT VVKP+E +L Sbjct: 123 IPY-QAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSIL 181 Query: 181 ELAALASEAGFPPGVINVVSGYGNEIGAALINHPRVAKIAFTGGDIGGQKVAEAAAKNFK 240 +A LA+E GFP G +N+V+GYG+E G L+ HP V I+FTG G+ V AAA++ Sbjct: 182 RVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHT 241 Query: 241 KITLELGGKSANIVFADADFEQAINGAIAGVFGAAGQTCMAGSRLLVERSIHDKFVARLI 300 +TLELGGKS IVF DAD E + + + AGQTC AGSRLLV+R +++ + RL Sbjct: 242 PVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLS 301 Query: 301 EVAGAARIGNPADSETQVGPIATRSQYEKILGYIEIAKQDGATCVLGGNALSGAQYGQGQ 360 + ++G+ + + GP+ ++Q E++ G++ A++DG G +G G G Sbjct: 302 GLFSQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTG-GF 359 Query: 361 FVAPTIFTNVRNDMRIAREEVFGPILSVIPFDSVEEALEIANDTDFGLAAAVWTTDLHKA 420 + AP + +V R+A+EEVFGP+L+ +PF+ A+ +AN TD+GL A+VWT D + Sbjct: 360 YQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQ 419 Query: 421 IYCSDRIEAGTVWVNNYRA-TSFTTPFGGYKRSGVGREGGLEGAQEYMQAKSVWI 474 + + I +G V++NNY A PFGG K SG GRE G E + K++ I Sbjct: 420 MRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 303 bits (777), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 187/476 (39%), Positives = 265/476 (55%), Gaps = 20/476 (4%) Query: 3 IGGVWQDSASGEWIDSIEPFSGDVIAQVPRGNEADAAQAVDAAYKAYRSPAWSELTATQR 62 I G WQ E + P + + + ++ GN AD QAV AA +A PAW+ + +R Sbjct: 20 IDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAI--PAWAATSPLER 77 Query: 63 GALLRKLGDLIERDAVRLADIERSCNGKNITEVSGQVRNVAQWFYYYA--GLADKIHGSV 120 ALL ++ L+ + A G I+ +A A LAD Sbjct: 78 AALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARDNLAD------ 131 Query: 121 VPINKPGVFNYVRYEPLGVVVAITPWNSPLALTAWKMAPALACGNTIVVKPSEFTSPPLL 180 P +P + EP+GV ITPWN P+ K+ PALA G T+V+KPSE + L Sbjct: 132 YPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSAL 191 Query: 181 ELAALASEAGFPPGVINVVSGYGNEIGAALINHPRVAKIAFTGGDIGGQKVAEAAAKNFK 240 A + EAG P GV N+V+G G E+GAAL HP+V I+ TG G VA+AAA K Sbjct: 192 LFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVK 251 Query: 241 KITLELGGKSANIVFADADFEQAINGAIAGVFGAAGQTCMAGSRLLVERS----IHDKFV 296 ++ ELGGKS N+V DAD E+AI +A GQ+C A +RL+V R+ +H+ + Sbjct: 252 RVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELAL 311 Query: 297 ARLIEVAGAARIGNPADSETQVGPIATRSQYEKILGYIEIAKQDGATCVLGGNALSGAQY 356 A L ++ ++G+P D+ T GP+A R+Q+ ++ IE QDGA + GG Sbjct: 312 ATLAQM----KVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDG-L 366 Query: 357 GQGQFVAPTIFTNVRNDMRIAREEVFGPILSVIPFDSVEEALEIANDTDFGLAAAVWTTD 416 QG + PTIF+ V DM IA+EE+FGP+L+++P+D+VEEA+ IANDT +GL A V D Sbjct: 367 AQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRD 426 Query: 417 LHKAIYCSDRIEAGTVWVNNYRATSFTTPFGGYKRSGVGREGGLEGAQEYMQAKSV 472 + + RI++G V + NY A PFGGYK+SG GRE G+EG +EYM+ K++ Sbjct: 427 KDQVRAVAARIQSGQVHL-NYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,568,087 Number of extensions: 66581 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 21 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)