BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0170.p (517 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 222 6e-59 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 196 4e-51 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 170 2e-43 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 153 4e-38 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 145 5e-36 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 222 bits (565), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 173/545 (31%), Positives = 267/545 (48%), Gaps = 51/545 (9%) Query: 6 LIAPVGELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFL 65 L+ + E +HA R AY +SY EL+ + L G LQ G+ G VAI + Sbjct: 25 LVQMIDEAFVQHADRQ----AYVCMGKYLSYRELDSLSRALGGWLQGLGLQKGARVAIMM 80 Query: 66 PNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVAALQAR 125 PN + + + AI+RAG V ++ T E+ ++++D+G +AIF ++ A + + A Sbjct: 81 PNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSGAEAIFVLENFACTLQKVVAH 140 Query: 126 FPFLKTVFLTEGASKAGGKDGLRYLDLR----------------------SAAAKSLPRE 163 V + G K L L +R A K+L Sbjct: 141 TNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSLPNAITFSQALSQARGKTLQPV 200 Query: 164 AEDIDQSSFIIYTSGTTGRAKGVLLTQRSML--------WVCAACWA--PIANLNENDRV 213 D +F+ YT GTTG AKG +LT R+++ W+ A A PI L Sbjct: 201 TLTHDDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEWLTPALKAGKPIDQL----VF 256 Query: 214 LSPLPLFHSYALNLSVLSILAVGASEYIM-DKFSTSETLALLKSGDFTVFPAVPTIFHYL 272 + LPL+H +AL + + GA ++ + + L FPAV T+++ L Sbjct: 257 VCALPLYHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKELAKYKVNTFPAVNTLYNAL 316 Query: 273 LEG---ARADPGCKFPGLRLCLSAGAIMPAPINKEFEERFGVMLLDGYGITETSTMVTLN 329 L AR D F G R C+ G + + ++++ G +++GYG++ETS + + N Sbjct: 317 LNNADFARLD----FSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASAN 372 Query: 330 WQHGTRVMGSCGLPLPGLATRIVDPSTGLDVESGQEGELIVRGPNLMKGYHRKPEETAKA 389 G+ GLPLP I+D G V GQ GE+ +RGP +M GY ++P+ETAK Sbjct: 373 PCTIDEYTGTIGLPLPSTEFAILD-DDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKV 431 Query: 390 LQ-NGWYYTGDLARSDQSGYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVG 448 + +G++ TGD+ D+ GY I R K++I+ G N+ P EVE V+ VL+CA VG Sbjct: 432 MTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVG 491 Query: 449 APHQHLGEVPVVFVVSRPGMTVVEADLLLHCKERLSSYKLPHAVHVVAEIPRTGSGKIIR 508 P +H GE +FVV R T+ A L+ +CKE+ + YK P + E+P+T GKI+R Sbjct: 492 VPDEHTGEAVKLFVV-RKDKTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILR 550 Query: 509 YKLRE 513 +LR+ Sbjct: 551 RELRD 555 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 196 bits (498), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 149/494 (30%), Positives = 234/494 (47%), Gaps = 20/494 (4%) Query: 34 VSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPNSVDWVESCFAIVRAGGVAVPISYDAT 93 + + E+ LA L G+ GD + + N+ ES +A + G V VP ++ T Sbjct: 63 IPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRIT 122 Query: 94 EHEVAYRIEDAGCKAIFTTDDKADVVAALQARFPFLKTVFLTEGASKAGGKDGLRYLDLR 153 E AY + +G + A V A++A P L+ V A +AG D Y L Sbjct: 123 PPEAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVALADA-RAGELD---YEALV 178 Query: 154 SAAAKSLPREAE-DIDQSSFIIYTSGTTGRAKGVLLTQRSMLWVCAACWAPIA-NLNEND 211 + + REAE D D + YTSGTTG K +L+ M +V A + L Sbjct: 179 REGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLMPGLTHQS 238 Query: 212 RVLSPLPLFHSYALNLSVLSILAVGASEYIM--DKFSTSETLALLKSGDFTVFPAVPTIF 269 R L PL H ++ + + A GA+ ++ ++ E L++ VPTI Sbjct: 239 RSLVVAPLSHGAGVHAVINT--ARGAASILLSSERLVVEEAWQLVEKYRVDNLFTVPTIV 296 Query: 270 HYLLEGARADPGCKFPGLRLCLSAGAIMPAPINKEFEERFGVMLLDGYGITETSTMVT-- 327 L+E D L+ + AGA M ++ G +L+ YG+ E + +T Sbjct: 297 KILVEDESVDR-YDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFL 355 Query: 328 ---LNWQ---HGTRVMGSCGLPLPGLATRIVDPSTGLDVESGQEGELIVRGPNLMKGYHR 381 L+++ H +G+CG+P G+ I++ G ++ + GE+ VRGP + GYH Sbjct: 356 PPYLHFEDDAHPQARVGTCGMPRTGMQIAILN-DDGQELAPFETGEICVRGPAVFMGYHN 414 Query: 382 KPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEAV 441 PE AKA ++ W++TGDL DQ G+L ITGR ++ I GG N+ P E+EEA+ AV Sbjct: 415 NPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPAV 474 Query: 442 LDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEADLLLHCKERLSSYKLPHAVHVVAEIPRT 501 + AV+G P GE + +V + G V LL H + R+ YK P +P++ Sbjct: 475 SEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLAHLESRIGKYKWPRRFIYWDSMPKS 534 Query: 502 GSGKIIRYKLRETL 515 G GKI++ +++ L Sbjct: 535 GYGKIVKKQIKALL 548 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 148/496 (29%), Positives = 220/496 (44%), Gaps = 24/496 (4%) Query: 32 TSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPNSVDWVESCFAIVRAGGVAVPISYD 91 TS SY +L+ + A L+ AG+ GD + I+L ++ V + A + G + VP+ Y Sbjct: 61 TSYSYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYL 120 Query: 92 ATEHEVAYRIEDAGCKAIFTTDDKADVVAALQARFPFLKTVFLTEGASKAGGKDGLRYLD 151 +AYR++++G A+ TD A L P L+ VF E +S + D Sbjct: 121 FGPDAIAYRLDNSGAVAL-VTDASGMEKATLAGELPALRLVFCVE-SSDLVLPQTTDFWD 178 Query: 152 LRSAAAKSLPREAEDIDQSSFIIYTSGTTGRAKGVLLTQRSMLWVCAACWAPIANL-NEN 210 AA L D + IIYTSGTTG+AKG L R +L + E Sbjct: 179 RLHAAPAELDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQEG 238 Query: 211 DRVLSPLPLFHSYALNLSVLSIL------AVGASEYIMDKFSTSETLALLKSGDF--TVF 262 DR +P +A +L +L V ++KF +E LL F Sbjct: 239 DRFWTPA----DWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFF 294 Query: 263 PAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPAPINKEFEERFGVMLLDGYGITET 322 P PT L A F LR S G + + E GV + + YG TE Sbjct: 295 P--PTALKMLRGAATVRAQADF-SLRSVASGGETLGDDLIAWGREALGVTINEFYGQTEC 351 Query: 323 STMVTLNWQHGTRVMGSCGLPLPGLATRIVDPSTGLDVESGQEGELIVRGPN--LMKGYH 380 + +V+ + V GS G +PG +IVD G + G G + +R P+ + Y Sbjct: 352 NLVVSSSSHCYPSVSGSMGRAVPGHVVQIVD-EQGQVLPHGTVGNIAIRAPDPVMFLRYW 410 Query: 381 RKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEA 440 R E T + + TGDL D+ GY+ GR ++I G I PA +EE + A Sbjct: 411 RNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPA 470 Query: 441 VLDCAVVGAPHQHLGEVPVVFVVSRPGMT---VVEADLLLHCKERLSSYKLPHAVHVVAE 497 V AVVG EV FVV + G+T ++A+L H + +L++++ P + VA Sbjct: 471 VRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAA 530 Query: 498 IPRTGSGKIIRYKLRE 513 +P T +GKI+R L+E Sbjct: 531 LPTTATGKIMRKTLKE 546 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 153 bits (386), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 142/520 (27%), Positives = 238/520 (45%), Gaps = 32/520 (6%) Query: 23 RKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPNSVDWVESCFAIVRAG 82 ++I Y R +SY + LA L++ G+ G++VA+ +S ++E FA+ G Sbjct: 32 QEIIYGQRR--LSYAGFYERVQRLANALKEMGLQPGNTVAVMDWDSNRYLECFFAVPMMG 89 Query: 83 GVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVAALQARFPFL-KTVFLTEGASKA 141 V ++ + ++AY + A + D ++ ++ L + V+LT+ A+ Sbjct: 90 CVLQTVNVRLSPEQIAYTLNHAQADVLLVNSDFLPLLKQIRGELTTLTRCVWLTDDATPC 149 Query: 142 GGKDGL--RYLDLRSAAAKSLPREAEDIDQSSFIIYTSGTTGRAKGVLLTQRSMLWVCAA 199 G Y +L + A + D + + YT+GTTG KGV + R ++ A Sbjct: 150 PRGPGFAGEYEELLAQAEPACDFPDFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLA 209 Query: 200 -----CWAPIANLNENDRVLSPL-PLFHSYALNLSVLSILAVGASEYIMDKFSTSETLAL 253 AP D V PL P+FH +A L ++ +A G + ++ L Sbjct: 210 TTAGLALAPQQGRFHRDDVYMPLTPMFHVHAWGLPYVATMA-GVKQVYPGRYVPDTICRL 268 Query: 254 LKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPAPINKEFEERFGVML 313 ++ T VPTI +L +A G ++ + G+ M + K +R G+ + Sbjct: 269 IREEKVTFSHCVPTILQMVLTSEQAREA-DLSGWKMII-GGSAMTGSLAKMARDR-GIDV 325 Query: 314 LDGYGITETSTMVTLNWQHGTRVMG---------SCGLPLPGLATRIVDPSTGLDVESGQ 364 GYG++ET ++TL Q T +G G PLP + R VD G Sbjct: 326 FTGYGMSETCPVLTLA-QVPTEELGGEREAAIRVKTGRPLPLVDLRTVDGEMQDCPRDGS 384 Query: 365 E-GELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELIIRGG 423 GE++VR P L +GY + E + + + GW +T D+ D GYL +T R+K++I GG Sbjct: 385 TTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGG 444 Query: 424 QNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVV-SRPGMTVVEADLLLHCKE- 481 + ++ ++E+ ++ AV + AV+G GE PV VV + V E D+ H + Sbjct: 445 EWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALVVLKKDAAAVSEDDIREHVRAA 504 Query: 482 ----RLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRETLPS 517 +S Y +P V VAE+ RT GK+ + +RE PS Sbjct: 505 AEAGAISRYGVPDRVRFVAELARTSVGKLNKRVMREQYPS 544 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 145 bits (367), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 147/533 (27%), Positives = 227/533 (42%), Gaps = 56/533 (10%) Query: 23 RKIAYEDRHTSVSYGELE-------KTTTNLAGHLQDAGIAAGDSVAIFLPNSVDWVESC 75 R A + T++ +G L + T LA LQ G+ D+V+ LPN+ VE+ Sbjct: 26 RAAAVYGKRTAIIHGALRQDWDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAH 85 Query: 76 FAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVAALQARFPFLKTV--F 133 F + AG V ++ + + + K + + A + L+ + P LK V F Sbjct: 86 FGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIVEVF 145 Query: 134 LTEGASKAGGK--DGLRYLDLRSAAAKSLPRE--AEDIDQSSFIIYTSGTTGRAKGVLLT 189 G G+ L Y L + + + A++ D + + YTSGTTG KGV+ Sbjct: 146 DELGPPPVAGERFGHLEYEALLAGGDEHFDWQMPADEWDAIA-LNYTSGTTGDPKGVVYH 204 Query: 190 QRSMLWVCAACWAPIANLNENDRVLSP-----LPLFHSYALNLSVLSILAVGASEYIMDK 244 R A ++N+ E D P LP+FH ++ A + K Sbjct: 205 HRG------AALNAVSNILEWDLPKHPVYLWTLPMFHCNGWCFP-WTVAARAGVNVCLRK 257 Query: 245 FSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSAGAIMPAPINKE 304 F L+ T + A P I H L A G + AGA PA + + Sbjct: 258 FEPKLVFDLIAEHGITHYCAAP-IVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAK 316 Query: 305 FEERFGVMLLDGYGITETSTMVTL-----NW-------------QHGTRVMGSCGLPLPG 346 E L YG+TE + W + G R G+ Sbjct: 317 MEA-MQFELTHVYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSRQGVRYHLQSGV---- 371 Query: 347 LATRIVDPSTGLDVESGQE--GELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSD 404 ++ P T V + E GE++ RG MKGY + T +A GW++TGDL + Sbjct: 372 ---TVLSPDTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFHTGDLGVMN 428 Query: 405 QSGYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVS 464 GY+ I R K++II GG+NI+ EVE+ + AVL AVV P + GE P FV Sbjct: 429 ADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVEL 488 Query: 465 RPGMTVVEADLLLHCKERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRETLPS 517 + G V A+L C+ L+ +K+P A++ +P+T +GKI +++LR+ + S Sbjct: 489 KEGAEVGAAELTEFCRSHLAGFKVPKAIY-FGPLPKTSTGKIQKFELRKRMKS 540 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,626,222 Number of extensions: 67966 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 20 Length of query: 517 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 428 Effective length of database: 1,198,455 Effective search space: 512938740 Effective search space used: 512938740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)