BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0169.p (491 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 715 0.0 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 481 e-137 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 375 e-105 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 334 1e-92 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 288 5e-79 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 715 bits (1845), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/483 (71%), Positives = 397/483 (82%), Gaps = 2/483 (0%) Query: 9 LKDPDLLRHQAFIGGTWSDADGGQVIEVTNPSTGKSLGTVPKMGRGETARAIDAAAKALR 68 LKDP LLR QAF+ G W DAD G+ + V NP+ G+ LG VP MG ET RAI+AA A Sbjct: 4 LKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWP 63 Query: 69 AWQSKTAKDRSTILRKWFDLLLANQDDLALIMTLEQGKPLAESKGEIAYAASFVEWFAEE 128 AW+ KTAK+RS ILR+W++L+LAN DDLALIMT EQGKPLAE++GEI YAASF+EWFAEE Sbjct: 64 AWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEE 123 Query: 129 GKRIYGDTIPAPSGGXSINIVVIKQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIK 188 GKR YGDTIP+PS S IVVIK+ +GVCAAITPWNFPAAMITRKA PALAAGC MV+K Sbjct: 124 GKRTYGDTIPSPS--PSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLK 181 Query: 189 PASMTPFSALALCVLAQRAGIPDGVLSCVTGSAGEIGAELTSNPTVRKLSFTGSTEIGKL 248 PA TPFSALAL VLA+RAGIP GV S VTG+ IG E+TSNP VRK+SFTGST +GKL Sbjct: 182 PAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKL 241 Query: 249 LLQQCAGTVKKTSMELGGNAPFIVFDDADIDAAVKGAMIAKYRNAGQTCVCANRILVQDG 308 L+QQ A ++KK S+ELGGNAPFIVFDDAD+DAAV+GA+ +KYRNAGQTCVCANRI VQDG Sbjct: 242 LMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDG 301 Query: 309 VYDVFVERFSAAVRALKVGDGLEQGVTIGPLIDDNAREKAEEHVADALKKGAKAVVGGAR 368 VYD F + AV+ KVG G E+GVT GPLI++ A +K E+HVADA+ KGA+ ++GG R Sbjct: 302 VYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKR 361 Query: 369 HALGGSFFQPTVLADATPDMAIFKEETFSPVAPVFRFHTEEEAVAMANDTEFGLASYFYG 428 HALG SFF+PTVLAD TP M + +EETF P+AP+FRF T+EE +A+ANDTEFGLASYFY Sbjct: 362 HALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYS 421 Query: 429 RDIGRIWRVAEALEYGMVGINEGLISTEVAPFGGIKESGSGREGSKYGITDYLETKYLCM 488 RDIGRIWRVAE LE GMVGIN GLIS EVAPFGG+K+SG GREGS YGI DYL KYLCM Sbjct: 422 RDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLCM 481 Query: 489 GGI 491 GGI Sbjct: 482 GGI 484 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 481 bits (1237), Expect = e-137, Method: Compositional matrix adjust. Identities = 264/493 (53%), Positives = 344/493 (69%), Gaps = 9/493 (1%) Query: 4 ISNIGLKDPDLLRHQAFIGGTWSDADGGQVIEVTNPSTGKSLGTVPKMGRGETARAIDAA 63 ++++ L+ DL+ IG W A G+ ++V++P+TG+ +VP G + A++AA Sbjct: 1 MTSLTLQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAA 60 Query: 64 AKALRAWQSKTAKDRSTILRKWFDLLLANQDDLALIMTLEQGKPLAESKGEIAYAASFVE 123 A AW++ AK R+ I+++W DLLLA+QDDL +++ EQGKPLAE+KGE+AYAAS+VE Sbjct: 61 VAAFAAWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVE 120 Query: 124 WFAEEGKRIYGDTIPAPSGGXSINIVVIKQPVGVCAAITPWNFPAAMITRKAAPALAAGC 183 WF EE R GD IPAP G ++ +K+PVGV AAITPWNFPAAMI RK APALAAGC Sbjct: 121 WFGEEATRANGDIIPAPVTGR--RMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGC 178 Query: 184 TMVIKPASMTPFSALALCVLAQRAGIPDGVLSCVTGS---AGEIGAELTSNPTVRKLSFT 240 T+V KPA TP ++LAL LAQ AG+P GV++ VT S E+ ++ VRK+SFT Sbjct: 179 TVVCKPAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFT 238 Query: 241 GSTEIGKLLLQQCAGTVKKTSMELGGNAPFIVFDDADIDAAVKGAMIAKYRNAGQTCVCA 300 GST +GK L + A T+KK S+ELGGNAPFIVFDDAD+DAA+ G M AK+RN GQTCV Sbjct: 239 GSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSP 298 Query: 301 NRILVQDGVYDVFVERFSAAVRALKVGDGLEQGVTIGPLIDDNAREKAEEHVADALKKGA 360 NRI VQ+ VYD FV++ A V ALKVG + IGP+I+ A K ++HV DA+ +GA Sbjct: 299 NRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGA 358 Query: 361 KAVVGGARHALGG----SFFQPTVLADATPDMAIFKEETFSPVAPVFRFHTEEEAVAMAN 416 + + GG R G +++ PTVLAD T M EETF PVAP+ RF TE+E +A AN Sbjct: 359 RVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAAN 418 Query: 417 DTEFGLASYFYGRDIGRIWRVAEALEYGMVGINEGLISTEVAPFGGIKESGSGREGSKYG 476 T FGLA+YFY D+ RI RV +ALE G+VG+NEG ++ E APFGG+KESG GREGS +G Sbjct: 419 ATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHG 478 Query: 477 ITDYLETKYLCMG 489 + DYL TKY+C G Sbjct: 479 LDDYLHTKYVCQG 491 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 375 bits (963), Expect = e-105, Method: Compositional matrix adjust. Identities = 198/470 (42%), Positives = 279/470 (59%), Gaps = 2/470 (0%) Query: 18 QAFIGGTWSDADGGQVIEVTNPSTGKSLGTVPKMGRGETARAIDAAAKALRAWQSKTAKD 77 Q FI G W+ + G+ I+V NP+T + +G + R + RA++AA+K W++ +A + Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 78 RSTILRKWFDLLLANQDDLALIMTLEQGKPLAESKGEIAYAASFVEWFAEEGKRIYGDTI 137 RS I+R+ DLL +++A +MT+EQGKPLAE+K E AA ++WFAEE +R YG + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 138 PAPSGGXSINIVVIKQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIKPASMTPFSA 197 PA G +VIK+PVG AA TPWNFP + RK + ALAAGC++++K TP S Sbjct: 126 PARVPGAYQ--MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 198 LALCVLAQRAGIPDGVLSCVTGSAGEIGAELTSNPTVRKLSFTGSTEIGKLLLQQCAGTV 257 L AG+P GV++ V G EI L +P ++K+SFTGST +GK L + Sbjct: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 258 KKTSMELGGNAPFIVFDDADIDAAVKGAMIAKYRNAGQTCVCANRILVQDGVYDVFVERF 317 K+ +MELGG+AP +VFDDAD+D A K AK+RNAGQ CV R LVQ GV++ FV++F Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 Query: 318 SAAVRALKVGDGLEQGVTIGPLIDDNAREKAEEHVADALKKGAKAVVGGARHALGGSFFQ 377 LKVG+GLE G+T+GP+ + E + DA+ +GA+ GG R G FFQ Sbjct: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQ 363 Query: 378 PTVLADATPDMAIFKEETFSPVAPVFRFHTEEEAVAMANDTEFGLASYFYGRDIGRIWRV 437 PTVL + EE F P+A + F T +E + AN +GLA+Y + + + Sbjct: 364 PTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHAL 423 Query: 438 AEALEYGMVGINEGLISTEVAPFGGIKESGSGREGSKYGITDYLETKYLC 487 A +E GM+ IN ++ PFGGIK+SG G EG I Y+ TK + Sbjct: 424 AARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 334 bits (856), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 197/476 (41%), Positives = 278/476 (58%), Gaps = 5/476 (1%) Query: 12 PDLLRHQAFIGGTWSDADGGQVIEVTNPSTGKSLGTVPKMGRGETARAIDAAAKALRAWQ 71 PDL Q +I G + +G + EV +P+TG LG +P R + AI AA +A + W+ Sbjct: 3 PDL---QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWR 59 Query: 72 SKTAKDRSTILRKWFDLLLANQDDLALIMTLEQGKPLAESKGEIAYAASFVEWFAEEGKR 131 + +RS ILR+ L +A +T++ GKPLA++ EI AA ++W AEEG+R Sbjct: 60 QVSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRR 119 Query: 132 IYGDTIPAPSGGXSINIVVIKQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIKPAS 191 +YG + A + +V+++PVGVCAAI+PWNFP +K A A+AAGCT+V+K S Sbjct: 120 LYGRVVGARH--PEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPS 177 Query: 192 MTPFSALALCVLAQRAGIPDGVLSCVTGSAGEIGAELTSNPTVRKLSFTGSTEIGKLLLQ 251 +P + + + L AG+P GV + V+G + EI L +P VR +SFTGS +G L Sbjct: 178 ESPAAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAA 237 Query: 252 QCAGTVKKTSMELGGNAPFIVFDDADIDAAVKGAMIAKYRNAGQTCVCANRILVQDGVYD 311 Q A +K+ SMELGG+AP +VFDDA+++ A + K RNAGQ CV +R VQ G+Y Sbjct: 238 QAALHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYA 297 Query: 312 VFVERFSAAVRALKVGDGLEQGVTIGPLIDDNAREKAEEHVADALKKGAKAVVGGARHAL 371 FV F+ A+ +KVG G E GV +GPLI E + + DAL +G++ V GGAR Sbjct: 298 RFVTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQ 357 Query: 372 GGSFFQPTVLADATPDMAIFKEETFSPVAPVFRFHTEEEAVAMANDTEFGLASYFYGRDI 431 G F+ PT++ P + +EE F P+A V F EEA+AMAN GLASY + + + Sbjct: 358 RGHFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSL 417 Query: 432 GRIWRVAEALEYGMVGINEGLISTEVAPFGGIKESGSGREGSKYGITDYLETKYLC 487 RV+ L+ GMV IN ++ PFGGI ESG G EG G +L TK + Sbjct: 418 DTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 288 bits (738), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 168/473 (35%), Positives = 257/473 (54%), Gaps = 20/473 (4%) Query: 20 FIGGTWSDADGGQVIEVTNPSTGKSLGTVPKMGRGETARAIDAAAKALRAWQSKTAKDRS 79 +I G W G + V P+T + + + + +A+ AA +A+ AW + + +R+ Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 80 TILRKWFDLLLANQDDLALIMTLEQGKPLAESKGEIAYAASFVEWFAEEGKRIYGDTIP- 138 +L + LLL + A +TLE G P I+YA + A E R+ D + Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMGAP-------ISYARTAHVPLAAEHLRVARDNLAD 131 Query: 139 ----APSGGXSINIVVIKQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIKPASMTP 194 P G +I +++P+GVCA ITPWN+P IT K PALAAGCT+V+KP+ ++P Sbjct: 132 YPFIRPRGTTAI----VREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSP 187 Query: 195 FSALALCVLAQRAGIPDGVLSCVTGSAGEIGAELTSNPTVRKLSFTGSTEIGKLLLQQCA 254 SAL + AG+P GV + V GS E+GA L+++P V +S TGST G L+ Q A Sbjct: 188 LSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAA 247 Query: 255 GTVKKTSMELGGNAPFIVFDDADIDAAVKGAMIAKYRNAGQTCVCANRILVQDGVYDVFV 314 TVK+ + ELGG +P +V DAD++ A+ + A RN GQ+C R++V Sbjct: 248 VTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVH 307 Query: 315 ERFSAAVRALKVGDGLEQGVTIGPLIDDNAREKAEEHVADALKKGAKAVVGGARHALG-- 372 E A + +KVGD + T GPL + + ++ + L+ GA+ + GG G Sbjct: 308 ELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLA 367 Query: 373 -GSFFQPTVLADATPDMAIFKEETFSPVAPVFRFHTEEEAVAMANDTEFGLASYFYGRDI 431 G + +PT+ ++ DM I +EE F PV + + T EEA+A+ANDT +GL ++ GRD Sbjct: 368 QGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDK 427 Query: 432 GRIWRVAEALEYGMVGINEGLISTEVAPFGGIKESGSGREGSKYGITDYLETK 484 ++ VA ++ G V +N + APFGG K+SG+GRE G+ +Y+E K Sbjct: 428 DQVRAVAARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVEGMEEYMEVK 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,472,390 Number of extensions: 61465 Number of successful extensions: 253 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 21 Length of query: 491 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 402 Effective length of database: 1,198,455 Effective search space: 481778910 Effective search space used: 481778910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)