BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0166.p (529 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 410 e-115 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 407 e-114 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 398 e-112 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 392 e-110 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 385 e-108 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 410 bits (1054), Expect = e-115, Method: Compositional matrix adjust. Identities = 242/431 (56%), Positives = 315/431 (73%), Gaps = 7/431 (1%) Query: 67 KLSEVRGKYGAAKALLTRLIDDDQGKELLAKLNASLAVAVPLNNQMIEMRLQHKNAEAIA 126 K +E GK + +++ +G+EL + + + +++ E+ Q K EA+ Sbjct: 94 KTTEALGK-------MEKILSTARGRELFQSIQEARSAYNTPRDKLRELIKQQKKEEAVE 146 Query: 127 FLMNKASPALRDTIVALDAIVSYETETTRRVADEAREDYLRTRNLLFALGAIAIILCAMI 186 L + PA LD V+++ + E R+ ++ L A+AI LC + Sbjct: 147 LLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEGRDTTQSAIAMMLGLSAVAIALCVIA 206 Query: 187 AWRITRSIKIPLTRALSVAEAVAAGDVSTKILSVAEDETGRLLSALKKMNDSLNRIVGRV 246 AW +TRSI PL A+SVA AVAAGD++ +I + ++DETG LL+ALK MNDSL+RIV V Sbjct: 207 AWLVTRSITRPLNEAVSVASAVAAGDLTVQIGATSKDETGALLAALKSMNDSLHRIVSEV 266 Query: 247 RSGAETIATASAEIAAGNRSLSDRTEQQAASLEETASSMEELTATVRQNADNARQANQLA 306 R G++TI TASAEIA GN LS RTE+QA +LEETAS+MEELT+TV+QNADNARQAN LA Sbjct: 267 RQGSDTINTASAEIATGNLDLSSRTEEQAGALEETASAMEELTSTVKQNADNARQANSLA 326 Query: 307 AAASEVAVKGGDVVSQVVATMASINDSSRKVVDIIAVIDGIAFQTNILALNAAVEAARAG 366 A ASEVAV+GG VV QVV TM IND+SRK+VDII+VIDGIAFQTNILALNAAVEAARAG Sbjct: 327 ATASEVAVQGGSVVGQVVQTMGEINDASRKIVDIISVIDGIAFQTNILALNAAVEAARAG 386 Query: 367 EQGRGFAVVASEVRALAQRSAGAAREIKTLIDDSVEKVGSGTKLVDSAGETMQEVVASVG 426 EQGRGFAVVASEVR LAQRSA AA+EIK LIDDSV +V +G++LV+ AG TM EVVASV Sbjct: 387 EQGRGFAVVASEVRTLAQRSASAAKEIKALIDDSVSRVDNGSRLVEQAGTTMTEVVASVR 446 Query: 427 KVRDIIEEIASASAEQTAGIEQIHQAVTQMDQTTQQNAALVEQASAASEALRDQAVQLTN 486 +V D++ EI++AS EQ+ GIEQI+ A+TQMDQ TQQNAALVE+A+AA+++L++Q+ +L+ Sbjct: 447 RVTDVVAEISAASNEQSDGIEQINHAITQMDQVTQQNAALVEEAAAAAQSLQEQSTRLSA 506 Query: 487 AVSIFKLGADE 497 VS+FKL + + Sbjct: 507 TVSVFKLSSHD 517 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 407 bits (1045), Expect = e-114, Method: Compositional matrix adjust. Identities = 226/339 (66%), Positives = 277/339 (81%) Query: 164 DYLRTRNLLFALGAIAIILCAMIAWRITRSIKIPLTRALSVAEAVAAGDVSTKILSVAED 223 DY R++L ALGAI I+L A +AW +TRSI PL +A+++AE VAAGD++++I S + D Sbjct: 182 DYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRD 241 Query: 224 ETGRLLSALKKMNDSLNRIVGRVRSGAETIATASAEIAAGNRSLSDRTEQQAASLEETAS 283 ETG+LL+ALK MN +L IVGRVR G ETI TAS EIA GN LS RTE QA SLEETAS Sbjct: 242 ETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETAS 301 Query: 284 SMEELTATVRQNADNARQANQLAAAASEVAVKGGDVVSQVVATMASINDSSRKVVDIIAV 343 +ME+LT+TV+QNADNARQAN+LAA+AS+VA +GG VV QVVATM +I+ SS+K+ DII V Sbjct: 302 AMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIGV 361 Query: 344 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAGAAREIKTLIDDSVEK 403 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA AA+EIK LIDDSVEK Sbjct: 362 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEK 421 Query: 404 VGSGTKLVDSAGETMQEVVASVGKVRDIIEEIASASAEQTAGIEQIHQAVTQMDQTTQQN 463 V G++LV AG+TMQEVV SV +V ++ EI AS EQ+ GIEQ++ A+T +D+TTQQN Sbjct: 422 VDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQN 481 Query: 464 AALVEQASAASEALRDQAVQLTNAVSIFKLGADEPSLMV 502 AALVEQA+AA+ +++DQA LT+AVS+F+L D L V Sbjct: 482 AALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAV 520 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 398 bits (1023), Expect = e-112, Method: Compositional matrix adjust. Identities = 251/482 (52%), Positives = 331/482 (68%), Gaps = 3/482 (0%) Query: 21 TARIIDDHNVKVSAANTATSAIRDVAAYASNIAMLDDDAAMEVERRKLSEVRGKYGAAKA 80 T I++D VKV+ T + A N M + + ++ + A Sbjct: 42 TDEIVNDRYVKVALGTQITDKVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLA 101 Query: 81 LLTRLIDDDQGKELLAKLNASLAVAVPLNNQMIEMRLQHKNAEAIAFLMNKASPALRDTI 140 + + + +G ELLA L + A +++++ Q K EA A L +K P Sbjct: 102 EMEKRTNTPRGSELLAALKQARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYF 161 Query: 141 VALDAIVSYETETTRRVADEAREDYLRTRNLLFALGAIAIILCAMIAWRITRSIKIPLTR 200 A+ +VS+++ + A+ A N++ L +A++L A+ A ITRSI PL Sbjct: 162 AAVKEMVSFQSSLMDKSAERASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNE 221 Query: 201 ALSVAEAVAAGDVSTKILSVAEDETGRLLSALKKMNDSLNRIVGRVRSGAETIATASAEI 260 A+ VA AVAAGD++ +I + ++DETG LL ALK MND+L+RIV VR+G +TI TAS EI Sbjct: 222 AVQVATAVAAGDLTVQIEATSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREI 281 Query: 261 AAGNRSLSDRTEQQAASLEETASSMEELTATVRQNADNARQANQLAAAASEVAVKGGDVV 320 A GN LS RTEQQA +LEETAS+MEELT+TVRQNADNARQAN LA AS+VA++GG VV Sbjct: 282 ATGNLDLSSRTEQQAGALEETASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVV 341 Query: 321 SQVVATMASINDSSRKVVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR 380 +VV TM IND+SRK+VDII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR Sbjct: 342 GEVVQTMGQINDASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR 401 Query: 381 ALAQRSAGAAREIKTLIDDSVEKVGSGTKLVDSAGETMQEVVASVGKVRDIIEEIASASA 440 LAQRSA AA+EIK+LID SVE+V SG++LV AG TM+EVVASV +V D++ EI +AS Sbjct: 402 TLAQRSAAAAKEIKSLIDASVERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASG 461 Query: 441 EQTAGIEQIHQAVTQMDQTTQQNAALVEQASAASEALRDQAVQLTNAVSIFKLGAD---E 497 EQ+ GIEQI+QA+ QMD+ TQQNAALVEQA+AA+++L++Q+ +L++ V IF+LG D + Sbjct: 462 EQSDGIEQINQAIVQMDEVTQQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQ 521 Query: 498 PS 499 PS Sbjct: 522 PS 523 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 392 bits (1008), Expect = e-110, Method: Compositional matrix adjust. Identities = 254/517 (49%), Positives = 345/517 (66%), Gaps = 47/517 (9%) Query: 2 LLILTLALSLNALREMQARTARIIDDHNVKVSAANTATSAIRDVAAYASNIAMLDDDAAM 61 +++LT+ L L A+RE++A V ++ + AT+ + + A S A L Sbjct: 20 VIVLTVILGLFAIRELKA----------VNQASTDMATNWLPTIKAAQSIQASLP----- 64 Query: 62 EVERRKLSEVR--------GKYGAAKALLTRL-------------IDDDQGKELLAKLNA 100 R ++SE++ + A KA+ +RL I + + K L K + Sbjct: 65 ---RMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFSK 121 Query: 101 SLAVAVPLNNQMIEMRLQHKNAEAIAFLMNKASPALRDTIVALDAIVSYETETTRRVADE 160 S A + ++ ++ + + K EA A ++ R I +D I+ ++R D Sbjct: 122 SFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDDM 181 Query: 161 AREDY----LRTRNLLFALGAIAIILCAMIAWRITRSIKIPLTRALSVAEAVAAGDVSTK 216 A + Y L LL A+ IA+ L M+A R++ PL A++VAE VA GD++ Sbjct: 182 AEQVYRSATLWIIGLLAAIVVIALGLAVMVA----RAVSRPLNEAVAVAERVAQGDLTMD 237 Query: 217 ILSVAEDETGRLLSALKKMNDSLNRIVGRVRSGAETIATASAEIAAGNRSLSDRTEQQAA 276 I + + DETG L+ +L+ MNDSL +IVG VR+G +TI TAS EIA GN LS RTE+QA Sbjct: 238 IRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAG 297 Query: 277 SLEETASSMEELTATVRQNADNARQANQLAAAASEVAVKGGDVVSQVVATMASINDSSRK 336 SLEETAS+MEELTATV+QNADNARQANQLAA+AS++A +GG VV QVV TM I +SS + Sbjct: 298 SLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNR 357 Query: 337 VVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAGAAREIKTL 396 + DII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA AA+EIK L Sbjct: 358 IADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQL 417 Query: 397 IDDSVEKVGSGTKLVDSAGETMQEVVASVGKVRDIIEEIASASAEQTAGIEQIHQAVTQM 456 IDDSVEKVG+G++LV+ AG TM EVVASV +V D++ EI++AS EQ+ GIE+I++A+TQM Sbjct: 418 IDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQM 477 Query: 457 DQTTQQNAALVEQASAASEALRDQAVQLTNAVSIFKL 493 D+ TQQNAALVE+A+AA+++L+DQA +L+ VS+FKL Sbjct: 478 DEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKL 514 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 236/435 (54%), Positives = 303/435 (69%), Gaps = 8/435 (1%) Query: 68 LSEVRGKYGAAKALLTRLIDDDQGKELLAKLNASLAVAVPLNNQMIEMRLQHKNAEAIAF 127 L EV +YG +L+ + + KEL ++ ++A+ + +M + Q + A A Sbjct: 97 LKEV-ARYG-------KLVSEPREKELYPEIERNIALYKEAHERMSALFEQDQKQAAHAL 148 Query: 128 LMNKASPALRDTIVALDAIVSYETETTRRVADEAREDYLRTRNLLFALGAIAIILCAMIA 187 + + ++P R + + ++ +A Y + + AL ++L ++ Sbjct: 149 MNSTSTPVYRKLQEQIGELSEVNSKGAALSDADADHIYGQANMAILALVTTCVVLAMALS 208 Query: 188 WRITRSIKIPLTRALSVAEAVAAGDVSTKILSVAEDETGRLLSALKKMNDSLNRIVGRVR 247 W I R + PL A+ +A VAAGD++ I + DETG+LL AL++MN +L IV VR Sbjct: 209 WWIARLVARPLANAVDIARQVAAGDLTADIPTAHRDETGQLLEALRQMNGNLLNIVREVR 268 Query: 248 SGAETIATASAEIAAGNRSLSDRTEQQAASLEETASSMEELTATVRQNADNARQANQLAA 307 G +TI TASAEIA GN LS RTEQQA +LEETAS+MEELT+TVRQN+DNARQANQLA Sbjct: 269 QGTDTITTASAEIATGNMDLSSRTEQQAGALEETASAMEELTSTVRQNSDNARQANQLAV 328 Query: 308 AASEVAVKGGDVVSQVVATMASINDSSRKVVDIIAVIDGIAFQTNILALNAAVEAARAGE 367 ASEVA GG VV +VVATM++INDSSRK+VDII VIDGIAFQTNILALNAAVEAARAGE Sbjct: 329 NASEVAQAGGTVVGKVVATMSAINDSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGE 388 Query: 368 QGRGFAVVASEVRALAQRSAGAAREIKTLIDDSVEKVGSGTKLVDSAGETMQEVVASVGK 427 QGRGFAVVASEVR LAQRSA AA EIK LIDDSV KV +G+KLVD AG+TM EVVASV + Sbjct: 389 QGRGFAVVASEVRTLAQRSAAAAHEIKALIDDSVAKVDTGSKLVDEAGQTMNEVVASVKR 448 Query: 428 VRDIIEEIASASAEQTAGIEQIHQAVTQMDQTTQQNAALVEQASAASEALRDQAVQLTNA 487 V DI+ EI +A AEQT GIEQI+ AV QMD+ TQQNAALVEQA+AA++AL++QA +L+ Sbjct: 449 VSDIVAEITAAGAEQTQGIEQINNAVVQMDENTQQNAALVEQAAAAAKALQEQAGRLSET 508 Query: 488 VSIFKLGADEPSLMV 502 VS+F+L A ++V Sbjct: 509 VSVFRLDAQHVPVVV 523 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.125 0.318 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,036,691 Number of extensions: 33008 Number of successful extensions: 498 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 72 Length of query: 529 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 439 Effective length of database: 1,193,720 Effective search space: 524043080 Effective search space used: 524043080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)