BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0165.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 464 e-132 gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC t... 142 4e-35 gi|300312953|ref|YP_003777045.1| amino acid ABC transporter perm... 135 4e-33 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 119 3e-28 gi|300313405|ref|YP_003777497.1| branched-chain amino acid ABC t... 107 2e-24 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 464 bits (1193), Expect = e-132, Method: Compositional matrix adjust. Identities = 240/323 (74%), Positives = 276/323 (85%) Query: 1 MNKILILVVALVLALVAPYVLYPVFLMKLLCFALFACAFNLLTGFTGLLSFGHAAFFGSA 60 MNK + + V L+L L AP+ +YPVFLMK+LCFALFACAFNLL G+TGLLSFGHAAF G A Sbjct: 1 MNKKIGIAVLLILLLAAPFGIYPVFLMKMLCFALFACAFNLLIGYTGLLSFGHAAFLGWA 60 Query: 61 GYVAGHALKVWGVPFEAGILIGTASAAAIAFVVGGLAIRRQGIYFTMITLALAQMLFFVF 120 GY+ G ALKVWG+P E G++IGT + A I V+G LAIRRQGIYFTMITLALAQMLFFV Sbjct: 61 GYMCGQALKVWGLPTELGLIIGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVA 120 Query: 121 LQAPFTGGEDGLQGIPRGKLLGSIDLNNDLTMYYLVVCIVSIAFALIVRTVHSPFGQILK 180 LQAPFTGGEDGLQG+PRGKLLG ++L +D+T+YY+V+ I FALIVRT+HSPFGQ+LK Sbjct: 121 LQAPFTGGEDGLQGVPRGKLLGFLNLESDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLK 180 Query: 181 AIKENEARAISLGYDVDRYKLLAFVLSGALAGLAGATKAVVLGFETLTDVHWSMSGLVVL 240 AIKENE RAISLGYDV RYKLLAFVLS L+GLAGATK +VLGFETLTDVHWSMSGLVVL Sbjct: 181 AIKENEPRAISLGYDVARYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVL 240 Query: 241 MTLVGGLGTKTGPIIGAILIISLENKLGELGDGLASLTGVEWFRALGESVTIVTGLIFIV 300 MTLVGGLGT GPI+GA++II LENKLG++G LAS+T ++WF LG+SVTIVTG IFIV Sbjct: 241 MTLVGGLGTILGPIVGAVIIIMLENKLGDIGAWLASVTHIDWFNTLGDSVTIVTGFIFIV 300 Query: 301 CVLAFRRGIVGEIGGLIRKLRAR 323 CVLAFR+GIVGEI L RK +A Sbjct: 301 CVLAFRKGIVGEILALTRKGQAS 323 >gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 334 Score = 142 bits (357), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 96/248 (38%), Positives = 141/248 (56%), Gaps = 5/248 (2%) Query: 28 KLLCFALFACAFNLLTGFTGLLSFGHAAFFGSAGYVAGHALKVWGVPFEAGILIGTASAA 87 ++L FAL A NLL G+TGL+SFG FFG Y AG L + + + A Sbjct: 32 EVLVFALAALGCNLLLGYTGLMSFGQGIFFGVGSYTAGVVLLQLKLALLPALALAALGGA 91 Query: 88 AIAFVVGGLAIRRQGIYFTMITLALAQMLFFV-FLQAPFTGGEDGLQGIPRGKL----LG 142 +A +VG +IR++G YF M+TLA AQM +F+ + TGG++GL IPR L L Sbjct: 92 LVALLVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTMKDVTGGDNGLLDIPRPPLALFGLT 151 Query: 143 SIDLNNDLTMYYLVVCIVSIAFALIVRTVHSPFGQILKAIKENEARAISLGYDVDRYKLL 202 + + Y +V + + F L+ R V S G+ L A++ENEARA +LGYDV KL Sbjct: 152 LLPTTSSWQYYTVVAILFVLVFWLLQRVVDSVLGKTLLAVRENEARASALGYDVRLLKLA 211 Query: 203 AFVLSGALAGLAGATKAVVLGFETLTDVHWSMSGLVVLMTLVGGLGTKTGPIIGAILIIS 262 AFV+SGA+ GLAG A++ G L+++ + S +++MT++GG G ++GA + Sbjct: 212 AFVISGAVTGLAGGLHAMMTGVAPLSNIEYHTSEAILVMTVIGGTGNLFASVLGASFYVL 271 Query: 263 LENKLGEL 270 + N L L Sbjct: 272 VGNWLSTL 279 >gi|300312953|ref|YP_003777045.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 317 Score = 135 bits (341), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 84/231 (36%), Positives = 136/231 (58%), Gaps = 16/231 (6%) Query: 26 LMKLLCFALFACAFNLLTGFTGLLSFGHAAFFGSAGYVAG--------HALKVWGVPFEA 77 L ++L FA+ A + N + G+ G++SFGHA FFG YV A +W Sbjct: 32 LTRILIFAMVAASLNFILGYGGMVSFGHAVFFGLGAYVTAIASFHGVTSAWLIW------ 85 Query: 78 GILIGTASAAAIAFVVGGLAIRRQGIYFTMITLALAQMLFFVFLQAPFTGGEDGLQGIPR 137 L+ A + V+G +A+R + +YF MIT+ALAQ+ ++ F+ + GG+DGLQ R Sbjct: 86 --LLAALVTALVGLVIGVIALRTRAVYFIMITMALAQLFYYFFVGFRYYGGDDGLQVTAR 143 Query: 138 GKLLGSIDLNNDLTMYYLVVCIVSIAFALIVRTVHSPFGQILKAIKENEARAISLGYDVD 197 L +D++ D Y++V+ ++ +F L+ R + S FG L AI++NE RA ++GY Sbjct: 144 PLLGFGLDMSGDNAFYWVVLAWLAASFILLQRLLASRFGLALNAIRQNERRAQAIGYPAL 203 Query: 198 RYKLLAFVLSGALAGLAGATKAVVLGFETLTDVHWSMSGLVVLMTLVGGLG 248 RYKL AFV++ +AG+AG+ + F + +HWS SG++++M +GG+G Sbjct: 204 RYKLAAFVIAAVIAGIAGSLIGMSNLFASPKLLHWSQSGILLVMVALGGVG 254 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 119 bits (299), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 87/258 (33%), Positives = 132/258 (51%), Gaps = 12/258 (4%) Query: 18 PYVLYPVFLMKLLCFALFACAFNLLTGFTGLLSFGHAAFFGSAGYVAGHALKVWGVPFEA 77 PY+ PV ++++L LFA + + L G G++SFGHAA+FG Y A P Sbjct: 345 PYL--PVLMVEILVAVLFAASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPL 402 Query: 78 GILIGTASAAAIAFVVGGLAIRRQGIYFTMITLALAQMLFFVFLQ-APFTGGEDGLQGIP 136 +L G +A A + G +R G+Y M+TLA AQ+++ Q TGG +GL G+ Sbjct: 403 AMLAGAGTAMLAALLFGWFCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLR 462 Query: 137 RGKLLGSIDLNNDLTMYYLVVCIVSIAFALIVRTVHSPFGQILKAIKENEARAISLGYDV 196 L S L YYL + ++ L+ R +H+PFG L+A ++ RA +LG DV Sbjct: 463 TAAALAS-----PLAYYYLALLCSALGVYLLRRVIHAPFGLALRAARDASGRAQALGMDV 517 Query: 197 DRYKLLAFVLSGALAGLAGATKAVVLG--FETLTDVHWSMSGLVVLMTLVGGLGTKTGPI 254 + F L+G GLAG A G + V+ S+ GLV M L+GG+ + GP+ Sbjct: 518 RALQWGGFALAGLFCGLAGVLFAFSKGSISPEVAGVNRSVDGLV--MVLLGGVQSLAGPL 575 Query: 255 IGAILIISLENKLGELGD 272 +GA + L++ + D Sbjct: 576 LGASVFTWLQDLVARETD 593 >gi|300313405|ref|YP_003777497.1| branched-chain amino acid ABC transporter ATP-binding protein/permease [Herbaspirillum seropedicae SmR1] Length = 596 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 88/267 (32%), Positives = 134/267 (50%), Gaps = 7/267 (2%) Query: 7 LVVALVLALVAPYVLYPVFLMKLLCF----ALFACAFNLLTGFTGLLSFGHAAFFGSAGY 62 L A+VLAL P + P F + LL + A+ A LLTG GL+SFG AAF G Y Sbjct: 14 LFFAIVLAL--PQLGLPPFWVTLLSYVGLYAIVALGLVLLTGVAGLISFGQAAFVGMGAY 71 Query: 63 VAGHALKVWGVPFEAGILIGTASAAAIAFVVGGLAIRRQGIYFTMITLALAQMLFFVFLQ 122 + +G+ G+L G A A AF++G L +R G Y + T+A L+++F Sbjct: 72 TTAYLTTQFGLSPWIGLLAGIALTACSAFLIGSLTMRISGHYLPLSTIAWGLSLYYLFGN 131 Query: 123 APFTGGEDGLQGIPRGKLLGSIDLNNDLTMYYLVVCIVSIAFALIVRTVHSPFGQILKAI 182 G DG+ GIP +GS++ N +MY L+ ++ +A +V + S G+ ++A+ Sbjct: 132 LEMLGKHDGINGIPV-ITIGSVEFGNGASMYLLIWVVLILALVSVVNFLDSRAGRAVRAL 190 Query: 183 KENEARAISLGYDVDRYKLLAFVLSGALAGLAGATKAVVLGFETLTDVHWSMSGLVVLMT 242 + ++G D R K++ FV + LAG++G A + T +LM Sbjct: 191 RFGTVMPEAMGVDTARLKIVIFVYAAVLAGISGWLYAHLQQAVNPTPFGLKYGIDYLLMV 250 Query: 243 LVGGLGTKTGPIIGAILIISLENKLGE 269 +VGG G G I+GA L+ L N L E Sbjct: 251 VVGGAGHVAGAILGAGLLAVLSNFLQE 277 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.146 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 744,187 Number of extensions: 27438 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 20 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)