BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0150.p (597 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 463 e-131 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 396 e-111 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 393 e-110 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 120 2e-28 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 105 1e-23 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 463 bits (1191), Expect = e-131, Method: Compositional matrix adjust. Identities = 242/535 (45%), Positives = 336/535 (62%), Gaps = 12/535 (2%) Query: 5 YNAPVKETLFLLEHCSTISELIKSGRCADLSFDLIEAVLTEAGKFASEILAPFNTVADRE 64 Y APVK+ LF++ + ++ + C D + + EA+L E KF SE++AP N AD+E Sbjct: 3 YTAPVKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADKE 62 Query: 65 GVSFVDGTVKTARGHRDIYREWTGAGWNGVSATVEYGGQGLPHAINTACLEYWHSACMSF 124 S+ DG V T+ G ++ +R++ AGW GV VE+GGQGLP + T C+E +SA +SF Sbjct: 63 PSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSF 122 Query: 125 GLGPLLSMGAADTIEAFGTQELRQKYLPELISGNWMATMNLTEAQAGSDLGALRTKAVAQ 184 L PLL+ G + + GTQ+ ++ ++ ISG W TMNLTE QAGSDL +RT+AV + Sbjct: 123 ALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPE 182 Query: 185 SDGSYRIFGSKIFITYGDHPLAENILHLVLARRPEAPPGSKGISLFLVPKLLLDAEGKST 244 DG+Y++FG+KIFITYG+H +AENI+HLVLAR P AP G KGISLF+VPK L++A+G Sbjct: 183 GDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLG 242 Query: 245 IRNDLRCTGIEHKLGLHGSPTCSMSFGDNDGAVGWLIGEEERGLAAMFTMMNRARLAVAA 304 RND+ C IEHKLG+ SPT + FGD+ GA+G L+GEE RGL MF MMN AR AV Sbjct: 243 ARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGI 302 Query: 305 QGVAIAEAATQHAFSYANERRQGLALEAKDRTVPSRIANHPDVARMLLNMKAMTSAARCI 364 QG++++E A Q A +YA +R Q L P I + PDV RML++M+A AAR + Sbjct: 303 QGLSVSERAYQKAVAYARDRIQSRDLAGSPG--PVAIIHQPDVRRMLMSMRAQIEAARAL 360 Query: 365 VIKTADAIDRARLCADMEDRQLAQDEADLLTPVAKAYSTDIGVEVASLGIQIRGGMGYVE 424 A D A D R+ Q + L P+ K + T++ ++V S G+Q+ GGMG++E Sbjct: 361 SYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIE 420 Query: 425 ESGAPQFLRDARIAPIYEGTNGIQAIDLVTRKLKIKDRDVVSRFLRTFLSIAKSV-ETCG 483 E+GA Q+ RDARI PIYEGT IQA DLV RK +D V++ + + K+V E G Sbjct: 421 ETGAAQYYRDARILPIYEGTTAIQANDLVGRKTA-RDGGAVAKGI--LAQVRKTVSELQG 477 Query: 484 QAGFGPAGAILTASIDSLEEATDFIQRKSADVSID---VLSGATNYLKLFGLTAG 535 A P A L + D+LE+ D++ A+V D V +G+ YLK+ G+ G Sbjct: 478 DAQLQPIAARLGEAADALEQVVDYV---VANVKSDIKAVFAGSVPYLKMAGVVLG 529 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 396 bits (1017), Expect = e-111, Method: Compositional matrix adjust. Identities = 212/471 (45%), Positives = 292/471 (61%), Gaps = 10/471 (2%) Query: 4 QYNAPVKETLFLLEHCSTISELIKS-GRCADLSFDLIEAVLTEAGKFASEILAPFNTVAD 62 QYNAP+++ F+L + E+ K AD + +L++ VL E GKFAS++L P N D Sbjct: 3 QYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGD 62 Query: 63 REGVSF--VDGTVKTARGHRDIYREWTGAGWNGVSATVEYGGQGLPHAINTACLEYWHSA 120 REG + TV T +G + Y+++ AGW +S E+GGQGLP IN + E +S+ Sbjct: 63 REGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSS 122 Query: 121 CMSFGLGPLLSMGAADTIEAFGTQELRQKYLPELISGNWMATMNLTEAQAGSDLGALRTK 180 ++ + P LS GA + + A G++E +Q YLP+L+SG W TM LTEA G+DLG LR+K Sbjct: 123 NQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSK 182 Query: 181 AVAQSDGSYRIFGSKIFITYGDHPLAENILHLVLARRPEAPPGSKGISLFLVPKLLLDAE 240 A Q+DGSY+I G+KIFI+ G++ ++ NI+HLVLAR P+AP GSKGISLFLVPK L +A+ Sbjct: 183 AEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNAD 242 Query: 241 GKSTIRNDLRCTGIEHKLGLHGSPTCSMSFGDNDGAVGWLIGEEERGLAAMFTMMNRARL 300 G RN + C IE K+G+HG+ TC M+ DGA GWLIG+ +GL AMF MN ARL Sbjct: 243 GSLGARNPIFCGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMNAARL 299 Query: 301 AVAAQGVAIAEAATQHAFSYANERRQGLAL---EAKDRTVPSRIANHPDVARMLLNMKAM 357 V QG+ + E A Q+A YA +R Q +L +A D+ I HPDV RMLL KA Sbjct: 300 GVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIV-HPDVRRMLLTAKAF 358 Query: 358 TSAARCIVIKTADAIDRARLCADMEDRQLAQDEADLLTPVAKAYSTDIGVEVASLGIQIR 417 R A +D+ D + R+ + D LLTPV KA+ TD S +Q+ Sbjct: 359 AEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVY 418 Query: 418 GGMGYVEESGAPQFLRDARIAPIYEGTNGIQAIDLVTRKLKIKDRDVVSRF 468 GG GY+ E G Q++RDARI IYEGTN IQ++DL+ RK+ + + + +F Sbjct: 419 GGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKF 469 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 393 bits (1009), Expect = e-110, Method: Compositional matrix adjust. Identities = 219/502 (43%), Positives = 305/502 (60%), Gaps = 17/502 (3%) Query: 1 MSSQYNAPVKETLFLLEHCSTISELIKS-GRCADLSFDLIEAVLTEAGKFASEILAPFNT 59 M+ Y P+++ F+L+ +++++ A++ DL+ VL EAG+FA++++AP N Sbjct: 1 MTMHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNG 60 Query: 60 VADREGVSFVDGTVKTARGHRDIYREWTGAGWNGVSATVEYGGQGLPHAINTACLEYWHS 119 + DREG F +GTV T G Y ++ GW ++ E+GGQGLPH ++ A E + Sbjct: 61 IGDREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA 120 Query: 120 ACMSFGLGPLLSMGAADTIEAFGTQELRQKYLPELISGNWMATMNLTEAQAGSDLGALRT 179 A + + P L GA + A G+ L+ +YL ++ SG W+ATM LTEAQAGSDLG LRT Sbjct: 121 ANHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRT 180 Query: 180 KAVAQSDGSYRIFGSKIFITYGDHPLAENILHLVLARRPEAPPGSKGISLFLVPKLLLDA 239 +AV Q DGSYR+ G KIFI+ G+H L NI+HLVLAR P+AP GSKG+SLFL P++L D Sbjct: 181 RAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDDG 240 Query: 240 EGKSTIRNDLRCTGIEHKLGLHGSPTCSMSFGDNDGAVGWLIGEEERGLAAMFTMMNRAR 299 RN + CTGIEHK+G+HGS TCS+ F + A WL+GE RGLAAMF MMN AR Sbjct: 241 S-----RNGVHCTGIEHKMGIHGSATCSLHF---EQAQAWLVGEAGRGLAAMFVMMNAAR 292 Query: 300 LAVAAQGVAIAEAATQHAFSYANERRQGLALEAKDRTVPSRIANHPDVARMLLNMKAMTS 359 L V AQG+ +AEAA Q A +YA ERRQ A + + I +HP V ++L+ + Sbjct: 293 LHVGAQGLGLAEAAYQRASAYARERRQSRAPGSSGNDL---IVHHPAVQKLLMEQRCRIE 349 Query: 360 AARCIVIKTADAIDRARLCADMEDRQLAQDEADLLTPVAKAYSTDIGVEVASLGIQIRGG 419 AR + +D A + R + + +TPV KA+ TD G + AS +Q+ GG Sbjct: 350 GARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGG 409 Query: 420 MGYVEESGAPQFLRDARIAPIYEGTNGIQAIDLVTRKLKIKDRDVVSRFLRTFLSIAKSV 479 GYV E+G QF+RDARI IYEGTN IQAID + RK+ + D R L FL + ++ Sbjct: 410 HGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKV-LGDE---GRRLDDFLGLVRA- 464 Query: 480 ETCGQAGFGPAGAILTASIDSL 501 + +G G +L ++D L Sbjct: 465 DIAEASGLGEQARLLGEAVDKL 486 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 100/310 (32%), Positives = 142/310 (45%), Gaps = 39/310 (12%) Query: 139 EAFGTQELRQKYLPELISGNWMATMNLTEAQAGSDLGALRTKAVAQSDGSYRIFGSKIFI 198 E +G+ ++++L L G + LTE GSD A+RT+A D + + G+K FI Sbjct: 97 EKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERDGD-DFILNGTKQFI 155 Query: 199 TYGDHPLAENILHLVLARRPEAPPGSKGISLFLVPKLLLDAEGKSTIRNDLRCTGIEHKL 258 T G H I+ V R G KGIS FLVP D G R+ E K+ Sbjct: 156 TSGKHA-GVAIVFAVTDR----SAGKKGISCFLVP---CDTPGFVVGRS-------EEKM 200 Query: 259 GLHGSPTCSMSFGDNDGAVGWLIGEEERGLAAMFTMMNRARLAVAAQGVAIAEAATQHAF 318 G H S T + + L+G+E G + + R+ +AAQ V +A AA + A Sbjct: 201 GQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAV 260 Query: 319 SYANERRQGLALEAKDRTVPSRIANHPDVARMLLNMKAMTSAARCIVIKTADAIDRARLC 378 SYA +R + VP I H V L +M + AAR +V + A D+ R C Sbjct: 261 SYARQR--------ESFGVP--IIEHQAVNFRLADMNTLLDAARLMVWRAAQLKDQGRPC 310 Query: 379 ADMEDRQLAQDEADLLTPVAKAYSTDIGVEVASLGIQIRGGMGYVEESGAPQFLRDARIA 438 EA + AK ++++ ++AS IQI GG+GY + + RD RI Sbjct: 311 LK---------EASM----AKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRIC 357 Query: 439 PIYEGTNGIQ 448 IYEG N IQ Sbjct: 358 QIYEGANDIQ 367 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 105 bits (262), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 121/432 (28%), Positives = 199/432 (46%), Gaps = 57/432 (13%) Query: 34 LSFDLIE--AVLTEA-GKFASEILAPFNTVADREGVSFVDGTVKTARGHRDIYREWTGAG 90 LSFD E A L EA FA +AP DR + + D++++ G Sbjct: 7 LSFDHGEDIAALREAVAAFAHSEIAPRAAEIDR-----------SDQFPMDLWKKLGDLG 55 Query: 91 WNGVSATVEYGGQGLPHAINTACLEYWHSACMSFGLGPLLSMGA-----ADTIEAFGTQE 145 G++ + EYGG GL + + +E A S GL S GA + I+ G +E Sbjct: 56 VLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGL----SYGAHSNLCVNQIKRNGNEE 111 Query: 146 LRQKYLPELISGNWMATMNLTEAQAGSDLGALRTKAVAQSDGSYRIFGSKIFITYGDHPL 205 ++KYLP LISG+++ + ++E AGSD+ +++ +A + D Y + GSK++IT G P Sbjct: 112 QKRKYLPRLISGDFIGALAMSEPNAGSDVVSMKLRADKKGD-RYVLNGSKMWITNG--PD 168 Query: 206 AENILHLVLARRPEAPPGSKGISLFLVPKLLLDAEGKSTIRNDLRCTGIEHKLGLHGSPT 265 A+ LV+ + + G++G++ FLV K +G S + KLG+ GS T Sbjct: 169 ADV---LVVYAKTDLEAGARGMTAFLVEK---GYKGFSVAQK-------LDKLGMRGSHT 215 Query: 266 CSMSFGDNDGAVGWLIGEEERGLAAMFTMMNRARLAVAAQGVAIAEAATQHAFSYANERR 325 + F D + ++G RG+ + + ++ R ++ + I +A Y ++R+ Sbjct: 216 GELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRK 275 Query: 326 QGLALEAKDRTVPSRIANHPDVARMLLNMKAMTSAARCIVIKTADAIDRARLCADMEDRQ 385 Q I + L +M + A + V A DRA AD + R Sbjct: 276 Q----------FGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRAD-SAD-KVRA 323 Query: 386 LAQDEADLLTPVAKAYSTDIGVEVASLGIQIRGGMGYVEESGAPQFLRDARIAPIYEGTN 445 L +D A + YS + +A IQ GG GY+ E + RDA++ I GT+ Sbjct: 324 LRKDAAGAIL-----YSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEIGAGTS 378 Query: 446 GIQAIDLVTRKL 457 I+ + L+ R+L Sbjct: 379 EIRRM-LIGREL 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,728,412 Number of extensions: 70285 Number of successful extensions: 238 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 9 Length of query: 597 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 506 Effective length of database: 1,188,985 Effective search space: 601626410 Effective search space used: 601626410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)