BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0149.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 260 1e-70 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 251 6e-68 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 240 1e-64 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 239 3e-64 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 218 1e-57 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 260 bits (665), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 157/421 (37%), Positives = 244/421 (57%), Gaps = 7/421 (1%) Query: 6 EKQLIGLVSKRLQWILLLMMFIAFMDRTNISFVASFMNKDIGLSARQFGWGASLFFVGYL 65 E + V +RL +LL ++++DR N+ F A MNKD+GL+ QFG GA LFF+GY Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 66 LFEIPSNLIMARVGARLWLARIMISWGIVSCASAFIVGPNSFYLARFLLGVAEAGFIPGV 125 FEIPSNL + R GAR+W++RIMISWG++S A+AF+VGP SF LARFLLG+AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 126 VLYLSYWVPHRYLGKFTGWFLMIVPISASLTAMLTAVLLGLDEVLGVTGWRWVFFTEGLP 185 LY + W P + GK T +FL+ +P++ + + L+ L+ L + G GW+ + E LP Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 Query: 186 AIVVGFFLLRYLADKPASATWLSTDQRTQLQAMVNADQ-VGKMTETPKLKPALTSPSVWC 244 A+++G L L D+PA A WLS D++ L+ ++ +Q V KLK A T+ V+ Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 259 Query: 245 LGGAYFFMNV-ALGAQTWYPLSIHLFKLSRPWETASIAIPSAIASLSMLLWAKRSDRGEE 303 L GA F + +L W P I F L AIP + +++M LWA+ ++R E Sbjct: 260 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSER 319 Query: 304 RSWYVIIPALVSALSWYGAAHWINDAFWLPALMSVAYSAMFAALVVFWTIPTRVLSVEAR 363 R ++V +++ALS G + +++ ++VA +++ + FW IP+ L+ A Sbjct: 320 RLFFVAGAIVLAALS-LGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAA 378 Query: 364 PAGLAIITALGLPGSMISLALGGQLRDQLGSFA-PCYLMAASGMLACGLVVVWMHLSDQR 422 GLA+I ++G G + ++ G +R+ F P +A + +L V+ + L D Sbjct: 379 AGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGA---VITLALGDPS 435 Query: 423 K 423 + Sbjct: 436 R 436 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 251 bits (642), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 158/437 (36%), Positives = 235/437 (53%), Gaps = 24/437 (5%) Query: 3 LQAEKQLIGLVSKRLQW----ILLLMMFIAFMDRTNISFVASFMNKDIGLSARQFGWGAS 58 +Q EK+L +++ W L+L AF+DR NI A MN D+GLS + FG+ +S Sbjct: 4 IQNEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASS 63 Query: 59 LFFVGYLLFEIPSNLIMARVGARLWLARIMISWGIVSCASAFIVGPNSFYLARFLLGVAE 118 LFF+ Y +FE+PSNL + + GAR WLARIMI+WG++S +A + GPNS Y RF+LG AE Sbjct: 64 LFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAE 123 Query: 119 AGFIPGVVLYLSYWVPHRYLGKFTGWFLMIVPISASLTAMLTAVLLGLDEVLGVTGWRWV 178 AGF PGVVLYL+YWVP Y + F++ +P ++ + + ++A+LL +D + G+ GW W+ Sbjct: 124 AGFFPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWL 183 Query: 179 FFTEGLPAIVVGFFLLRYLADKPASATWLSTDQRTQLQAMVNADQVGKMTETP-KLKPAL 237 F EGLP +V+G L +L D+P A WLS +QR L +N + + + L Sbjct: 184 FMLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLF 243 Query: 238 TSPSVW-------CLGGAYFFMNVALGAQTWYPLSIHLFKLSRPWETASIAIPSAIASLS 290 + P VW C A +NV W P + F LS +IP IAS+ Sbjct: 244 SQPHVWGMALVCACASAAGSVLNV------WQPQLLKSFGLSVMQTGLVNSIPYLIASVL 297 Query: 291 MLLWAKRSDRGEERSWYVIIPALVSALSWYGAAHWINDAFWLPALMSVAYSAMFAALVVF 350 M+ W + SDR ER W+ IP ++ A N + L+++ ++ F Sbjct: 298 MVWWGRHSDRTGERRWHTAIPLMLIG-GGLALASLANSLAPVVLLLTMVLIGAYSFKGPF 356 Query: 351 WTIPTRVLSVEARPAGLAIITAL-GLPGSMISLALGGQLRDQLGSFAPCYLMAASGMLAC 409 W + + L+ + AGLA I A+ L G + + + G ++D GS+A MA M+ Sbjct: 357 WAMASSWLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYA----MALLPMVGL 412 Query: 410 GLVVVWMHLSDQRKKVA 426 +V V + L RK A Sbjct: 413 SVVSVGILLFLSRKSRA 429 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 240 bits (613), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 144/398 (36%), Positives = 228/398 (57%), Gaps = 10/398 (2%) Query: 15 KRLQWILLLMMFI----AFMDRTNISFVASFMNKDIGLSARQFGWGASLFFVGYLLFEIP 70 ++ W LL ++F+ +++DR N+ F M +D+ S +G GA +FF+GY +FE+P Sbjct: 22 SKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVP 81 Query: 71 SNLIMARVGARLWLARIMISWGIVSCASAFIVGPNSFYLARFLLGVAEAGFIPGVVLYLS 130 SN+I+ +VGARLW+ARIMI+WGI+S A A++ P FY+ RFLLGVAEAGF PGV+LYL+ Sbjct: 82 SNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLT 141 Query: 131 YWVPHRYLGKFTGWFLMIVPISASLTAMLTA-VLLGLDEVLGVTGWRWVFFTEGLPAIVV 189 YW P GK T F+ + +S + L+ ++ + V G TGW+W+F E +P++++ Sbjct: 142 YWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLIL 201 Query: 190 GFFLLRYLADKPASATWLSTDQRTQLQAMVNADQVGKMTETPKLKPALTSPSVWCLGGAY 249 G ++ Y+ D+ A WLS +++ L+A ++ ++ K E L + VW Y Sbjct: 202 GVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQK--EHLSLGAMFANGRVWLGALIY 259 Query: 250 FFMNVAL-GAQTWYPLSIHLFKLSRPWETASI-AIPSAIASLSMLLWAKRSDRGEERSWY 307 F + L G W P I +S P + AIP A A+ +M+L + +D ER W+ Sbjct: 260 FCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWH 319 Query: 308 VIIPALVSALSWYGAAHWINDAFWLPALMSVAYSAMFAALVVFWTIPTRVLSVEARPAGL 367 V IPA + + + + ++ A +++A + L +FW++PT LS A AG+ Sbjct: 320 VAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGI 379 Query: 368 AIITALGLPGSMISLALGGQLRDQLGSF-APCYLMAAS 404 A+I +LG +S L G L+D S A Y++AAS Sbjct: 380 ALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAAS 417 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 239 bits (611), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 141/391 (36%), Positives = 221/391 (56%), Gaps = 7/391 (1%) Query: 6 EKQLIGLVSK---RLQWILLLMMFIAFMDRTNISFVASFMNKDIGLSARQFGWGASLFFV 62 +K GL K R+ L+L IA++DR N+ F M++D+G S FG GA LFF+ Sbjct: 15 DKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFI 74 Query: 63 GYLLFEIPSNLIMARVGARLWLARIMISWGIVSCASAFIVGPNSFYLARFLLGVAEAGFI 122 GY LFE+PSN++M +VGAR+W+ARIMI+WGI+S A F+ FY+ RFLLG+AEAGF Sbjct: 75 GYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFY 134 Query: 123 PGVVLYLSYWVPHRYLGKFTGWFLMIVPISASLTAMLTA-VLLGLDEVLGVTGWRWVFFT 181 PG++LYL+YW P K F+ +P++ L L+ ++ + G+ GW+W+F Sbjct: 135 PGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLI 194 Query: 182 EGLPAIVVGFFLLRYLADKPASATWLSTDQRTQLQAMVNADQVGKMTETPKLKPALTSPS 241 E +PA+++G + YL + SA WL+ +++ LQA ++ D GK ++ + Sbjct: 195 EAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKH-GFGAIVKDAR 253 Query: 242 VWCLGGAYF-FMNVALGAQTWYPLSIHLFKLSRPWETASI-AIPSAIASLSMLLWAKRSD 299 VW + YF F+ G W P + + E + AIP A ++M L + +D Sbjct: 254 VWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSAD 313 Query: 300 RGEERSWYVIIPALVSALSWYGAAHWINDAFWLPALMSVAYSAMFAALVVFWTIPTRVLS 359 R ER W+++IPAL+ + + GAA + ++ A +S+A + + +FW++PT LS Sbjct: 314 RMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLS 373 Query: 360 VEARPAGLAIITALGLPGSMISLALGGQLRD 390 A G+A I ++G +S L G L+D Sbjct: 374 GAAAAVGIAAINSVGNLAGFVSPYLIGYLKD 404 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 218 bits (554), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 138/399 (34%), Positives = 217/399 (54%), Gaps = 9/399 (2%) Query: 4 QAEKQLIGLVSKRLQWILLLMMFIAFMDRTNISFVASFMNKDIGLSARQFGWGASLFFVG 63 QAE + V R+ L + ++F+DR NI F M +D+G S +G GA++F+VG Sbjct: 19 QAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVG 78 Query: 64 YLLFEIPSNLIMARVGARLWLARIMISWGIVSCASAFIVGPNSFYLARFLLGVAEAGFIP 123 Y+LFE+PSN+ +AR GAR +RIM+ WG+ S + FY RFLLGV EAGF P Sbjct: 79 YVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFP 138 Query: 124 GVVLYLSYWVPHRYLGKFTGWFLMIVPISASLTAMLTA-VLLGLDEVLGVTGWRWVFFTE 182 G+VLYLSYW P R F V ++ L +++ ++ + V+G+ GW+W+F E Sbjct: 139 GIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIE 198 Query: 183 GLPAIVVGFFLLRYLADKPASATWLSTDQRTQLQAMVNADQVGKMTETPK----LKPALT 238 G PA+V+G + LAD PA A WLS ++R+ LQA + + + L+ AL+ Sbjct: 199 GAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQALS 258 Query: 239 SPSVWCLGGAYFFMNV-ALGAQTWYPLSIHLFKLSRPWETASIA-IPSAIASLSMLLWAK 296 + + YF + +L W PL I F + E + + IP+AI ++ ++L A+ Sbjct: 259 HRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIAR 318 Query: 297 RSDRGEERS-WYVIIPALVSALSWYGAAHWINDAFWLPALMSVAYSAMFAALVVFWTIPT 355 RSDR ER + + + H + A L L+SVA +FAAL +FW++P Sbjct: 319 RSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSL-GLLSVAAVLIFAALPIFWSLPP 377 Query: 356 RVLSVEARPAGLAIITALGLPGSMISLALGGQLRDQLGS 394 +L + G+A I+++G+ ++S L G ++ + G+ Sbjct: 378 GLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGN 416 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.138 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,366,196 Number of extensions: 58538 Number of successful extensions: 317 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 17 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)