BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0146.p (495 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 181 2e-46 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 116 5e-27 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 96 8e-21 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 181 bits (458), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 155/530 (29%), Positives = 243/530 (45%), Gaps = 63/530 (11%) Query: 1 MQANYDVLISGYGPVGATLATLLAQQGLKVAILEREPGIYHLARAGHLDAEVMRVFQAIG 60 MQA VLI G GPVG TL LL G+ ++ER + RA +D E +R FQ+ G Sbjct: 15 MQAQ--VLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSAG 72 Query: 61 VAEELEKQCAVTLGMRFLDADGALLMEWKRGGEKGALGWVSDYMFHQPTLERILRAKVNA 120 +A+ + + + MR AD E + + GW +F QP E LR + Sbjct: 73 MADAIVRDVIQNVPMRMYTADRRCFAEIQPTTRE--FGWYRRNLFSQPLGEATLRLGLRR 130 Query: 121 SSDIDVYLQHDVYAIENREDAVIVKA---EDHRSGRLVKLTGRYLVGCDGARSLVR-RTM 176 + L+ V ++ +D V+A ++H G L +Y+VGCDG RS VR + + Sbjct: 131 FEHVS--LRSGVELLDLVQDEAGVQASIIDEH--GAQATLRAQYVVGCDGGRSTVREKIL 186 Query: 177 EVEQEDLGFKQKWLVIDVEEKRDLGLERVSTQHCNPERPMYASCTSDKMLRWEIMVKPED 236 ++ E KW+VI+ + D + HC P+RP + RWE M+ P + Sbjct: 187 KLPYEGKTHPAKWVVIECDG--DPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGE 244 Query: 237 EIHHITKPDVIWDFIENSMRPIRREDGQLMRSAVYTFESLIARTWRRGRMFIAGDAAHRM 296 + + P+ + + + +R + + ++R+ VYT S +A + GR+ +AGDAAH Sbjct: 245 DGEQMLAPEKVRELLREHVRDV--DALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHIT 302 Query: 297 PPFLGQGMCAGIRDASNLAWKLAAVCQGRADDTLLDTYESERRPHLKVFTE-----GAIE 351 PP++GQG+ AG+RDA NLAWKLA V QGR LL +Y ER H+K + GA+ Sbjct: 303 PPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGAVL 362 Query: 352 AGK-----------FVQMRDPVELERRVRDMRDNPKAYAPPNPPLGPGLQSRHGESGLGR 400 + K F+ ++D + V M+ P + +++R +GR Sbjct: 363 SQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFKPMPSFSSQAVMQDDVRNR--SDLVGR 420 Query: 401 QFVQPAVRGSL-----LDSMAGHAFALV------------GTEQFMEQQTIAHLRSAH-- 441 F+Q V LD + G F L+ G M+ +L+ Sbjct: 421 MFLQSLVENGAGEVMRLDDLCGGRFVLLSWRCDVLAAAEPGLRSRMQAFGCDYLQGVRSR 480 Query: 442 --PSLQLIA----LEEGQQDLLLPW----GAAAVLMRPDRYVHGAVAAVD 481 P Q++A + + ++ L W G VL+RPDR++ A A D Sbjct: 481 SGPRGQVLAGAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFIAAAGRAGD 530 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 116 bits (290), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 98/357 (27%), Positives = 162/357 (45%), Gaps = 50/357 (14%) Query: 5 YDVLISGYGPVGATLATLLAQQGLKVAILEREPGIYHLARAGHLDAEVMRVFQAIGVAEE 64 + V+I G GPVG A LA+QG+ V +++ + + +RA + +F IG + Sbjct: 31 HPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLDIFDRIGCGQR 90 Query: 65 L-EKQCAVTLGMRFLDADGALLMEW--KRGGEKGALGWVSDYMFHQPTLERILRAKVNAS 121 + +K G FL + + G + A + Y +E L + Sbjct: 91 MVDKGVGWNRGKVFLRDQAVYEFDLLPESGHARPAFINLQQYY-----VEGYLLEQAQQW 145 Query: 122 SDIDVYLQHDVYAIENREDAVIVKAEDHRSGRLVKLTGRYLVGCDGARSLVRRTMEVEQE 181 I++ ++ V AI + ED + + + +K RY+V DG+RS +R+ M +E + Sbjct: 146 PHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLK--ARYVVAADGSRSPLRKMMGLESQ 203 Query: 182 DLGFKQKWLVIDVEEKRDLGLERVSTQHCNPERPMYASCTSDKMLRWEIMVKPEDEIHHI 241 F+ ++L+ DV+ M A +++ ++ P+ + Sbjct: 204 GHTFRDRFLIADVK--------------------MDAPFPAERWFWFDPPFHPQQSVLLH 243 Query: 242 TKPDVIW--DF-------------IENSMRPIRREDGQ-----LMRSAVYTFESLIARTW 281 +PD +W DF E + +R G+ L +VYTF + + Sbjct: 244 RQPDDVWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERF 303 Query: 282 RRGRMFIAGDAAHRMPPFLGQGMCAGIRDASNLAWKLAAVCQGRADDTLLDTYESER 338 R GR+ GDAAH + PF +G +GI+DA NLAWKLA V QG+A D+LLD+Y SER Sbjct: 304 RHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 95.5 bits (236), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 87/345 (25%), Positives = 149/345 (43%), Gaps = 22/345 (6%) Query: 3 ANYDVLISGYGPVGATLATLLAQQGLKVAILEREPGIYHLARAGHLDAEVMRVFQAIGVA 62 + + V++ G GP+G T A A +GL V +L+ + +RA + ++ +GVA Sbjct: 24 SRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPLEIWDRLGVA 83 Query: 63 EEL-EKQCAVTLGMRFLDADGALLMEWKRGGEKGALGWVSDYMFHQPTLERILRAKVNAS 121 + EK + LG F + + ++ Q LE+ L A Sbjct: 84 PRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMIN---LQQYYLEQYLVEACQAQ 140 Query: 122 SDIDVYLQHDVYAIENREDAVIVKAEDHRSGRLVKLTGRYLVGCDGARSLVRRTMEVEQE 181 ID+ +H + ++ + + E + ++ +++ CDGA S R+ + E Sbjct: 141 PLIDLRWKHKMISLHQDDTHATLDVETPDG--IFRMEADWVIACDGANSDTRKMVGAEFS 198 Query: 182 DLGFKQKWLVIDVEEKRDLGLERV----STQHCNPERPMYASCTS----DKMLRWEIMVK 233 F+ ++L+ DV K D ER H ++ C + D L WE Sbjct: 199 GQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCDNVWRIDFQLGWE--AD 256 Query: 234 PEDEIHHITKPDVIWDFIENSMRPIRREDGQLMRSAVYTFESLIARTWRRGRMFIAGDAA 293 PE+E KP+ + I+ + + + +L +VY F R R+ AGDAA Sbjct: 257 PEEE----KKPEKVIPRIQAMLG--KEVEFELEWVSVYQFACRRIDKMRYQRVLFAGDAA 310 Query: 294 HRMPPFLGQGMCAGIRDASNLAWKLAAVCQGRADDTLLDTYESER 338 H++ PF +G G++D NL WKL V G+A + +D+Y ER Sbjct: 311 HQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEER 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,597,447 Number of extensions: 69037 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 3 Length of query: 495 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 406 Effective length of database: 1,198,455 Effective search space: 486572730 Effective search space used: 486572730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)