BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0139.p (355 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 97 1e-21 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 94 2e-20 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 90 3e-19 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 86 6e-18 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 84 1e-17 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 110/377 (29%), Positives = 171/377 (45%), Gaps = 54/377 (14%) Query: 24 NVTISGVLGAGVSYTNNASYPNGGGRMQQGNTHAVPF--IAFTGREDLGGGAFAIFKWAN 81 +VT+ G + G++Y++N GG N+ A+ + TG EDLGGG A+F+ N Sbjct: 36 SVTLYGTVDLGLAYSSN----QGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLEN 91 Query: 82 YVQLDTGGFSPF------ETFVGLNSRDWGTVTLGNQY----DLLADLVPYTSERFTSNL 131 DTG S +++VGL+S+D+G VT+G QY + L P + T Sbjct: 92 GFNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGP--TNVLTGAT 149 Query: 132 ATHPGN---LDRTVGNPLNSLVKYKSPELNGFQFGAQYGYGTPTSTTNNGNTMGFSASYS 188 HPG+ LD T+ +N+ + Y P L G Q G QYG G + NG+++ + Y Sbjct: 150 GAHPGDIDALDTTL--RMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYD 207 Query: 189 SNPWQFLAVWETVHDVPY-SPRSSLGVPTLYGIDLVKNPTTKVAQNQNTASAGVVYSDAG 247 + + A + + ++ + S G + + N A + + Y+ Sbjct: 208 YQAFSWSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAARYTRGD 267 Query: 248 WRLMSNYSYTKLSALGVSAKAQSID------LGAYKYVTTALRLGGGYGYT--KLEN--- 296 L NYS + A +SA +Q+ +G+Y V+ AL L GY YT K N Sbjct: 268 LMLGLNYSNVQYKAGALSAFSQTAVFNTIGLIGSYN-VSPALVLAAGYSYTAEKARNGIS 326 Query: 297 --YKWNMVHAHADYALSKRTSLYALSVFQ-AAGQ----GQVAAMRLQPIASTNR------ 343 K+N + Y+LSKRT+LYA+ +Q A+GQ G V + + +AS Sbjct: 327 SPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSSIVNAVASVGDSQNGTP 386 Query: 344 -----QVVFEVGITHVF 355 Q V +GI H F Sbjct: 387 SSGSGQFVAMLGIRHSF 403 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 106/361 (29%), Positives = 155/361 (42%), Gaps = 59/361 (16%) Query: 24 NVTISGVLGAGVSYTNNASYPNGGG---RMQQGNTHAVPFIAFTGREDLGGGAFAIFKWA 80 +VTI G++ GV Y NNA PNG G RM G T+ I F G EDLGGG A+F+ Sbjct: 24 SVTIYGLVDTGVEYLNNAG-PNGAGSVTRMTSGATN-TSRIGFRGTEDLGGGLKALFQLE 81 Query: 81 NYVQLDTGGFSP-------FETFVGLNSRDWGTVTLGNQYDLLADLV-PYTSERFTSNLA 132 N +LD G F ++ VGL +G V G Y D + P+ +++N + Sbjct: 82 NGFKLDNGSFDGDANQLFNRQSTVGLEG-GFGRVVAGRSYSTTYDFLGPFDPMGYSANYS 140 Query: 133 ---THPGNLDRTVGNP--LNSLVKYKSPELNGFQFGAQYGYGTPTSTTNNGNTMGFSASY 187 T +R G P +++LVKY+ E +G + GA YG+G T NG G + Sbjct: 141 WATTAGATGNRKDGMPTGVSNLVKYQG-EFSGVKIGAMYGFGEVAGETRNGAKYGVGLGW 199 Query: 188 SSNPWQFLAVWE-----TVHDVPYSPRSSLGVPTLYGIDLVKNPTTKVAQNQNTASAGVV 242 + P+ +A ++ T + PY +S + +AG Sbjct: 200 GTGPFSVVATYDRNNSATATNTPYDRATSAHL-----------------------AAGYQ 236 Query: 243 YSDAGWRLMSNYSYTKLSALGVSAKAQSIDL----GAYKYVTTALRLGGGYGYTKLENYK 298 S+A W+ Y Y K S L A D AYK+ T A+ L G Y ++N Sbjct: 237 PSEA-WKFNLGYRYYKKS-LATGAADLRSDFFWGGAAYKF-TPAMTLIGAIYYQNIKNVA 293 Query: 299 WN----MVHAHADYALSKRTSLYALSVFQAAGQGQVAAMRLQPIASTNRQVVFEVGITHV 354 + M YA+SKRT LY + + A GQ+ + + Q +G+ H Sbjct: 294 NDADPIMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAGYGSSQFGMTLGMQHR 353 Query: 355 F 355 F Sbjct: 354 F 354 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 108/388 (27%), Positives = 158/388 (40%), Gaps = 70/388 (18%) Query: 2 LKKTLFAGAVTLVAAS-AHGQTQNVTISGVLGAGVSYTNNASYPNGGG----RMQQGNTH 56 +KKTL A+ A+ AH Q+ +V+I G++ G++Y ++A GGG RM G Sbjct: 1 MKKTLLVAAMLAGGATTAHAQS-SVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQ 59 Query: 57 AVPFIAFTGREDLGGGAFAIFKWANYVQLDTGGFSP------FETFVGLNSRDWGTVTLG 110 I F G EDLGGG A F D G ++FVGL S WG++++G Sbjct: 60 GSR-IGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVG 118 Query: 111 NQYDLLAD---------LVPYTSERFTSNL---ATHPGNLDRTV--GNPLNSLVKYKSPE 156 QYD +A+ L P S F L A H L+ + G+ N+ +KY S + Sbjct: 119 RQYDFMANIGGQYAMGALSPAGS--FGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAK 176 Query: 157 LNGFQFGAQYGYGTPTSTTNNGNTMGFSASYSSNPWQFLAVWETVHDVPYSPRSSL-GVP 215 GF FGA G G N G T+ + Y + P+ + + D S + + G Sbjct: 177 FGGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFSTRIYGGG 236 Query: 216 TLYGIDLVKNPTTKVAQNQNTASAGVVYSDAGWRLMSNYSYTKLSALGVSAKAQSIDLGA 275 Y I P V Q +NT + SAKA + +LG Sbjct: 237 ARYQIGAF-TPFGVVTQVKNTNT--------------------------SAKATTYELGT 269 Query: 276 YKYVTTALRLGGGYGYTKLENYKWN--MVHAHADYALSKRTSLYALSVFQAAGQGQVAAM 333 +T L Y + K N + A DY +SKRT LY +V+ + Sbjct: 270 VYALTPLWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYD-----RDKGY 324 Query: 334 RLQPI------ASTNRQVVFEVGITHVF 355 P+ ++T Q +G+ H F Sbjct: 325 NAYPVFGGGIQSATGNQTALRLGMRHRF 352 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 85.5 bits (210), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 69/208 (33%), Positives = 96/208 (46%), Gaps = 20/208 (9%) Query: 2 LKKTLFAGAVT-LVAASAHGQTQNVTISGVLGAGVSYTNNASYPNGGGRMQQGNTHAVP- 59 +KK+L A AV A +AH Q+ ++TI G + A ++YTN + G+ AV Sbjct: 1 MKKSLLALAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKIATDG----KNTGSLTAVES 56 Query: 60 ------FIAFTGREDLGGGAFAIFKWANYVQLDTGGFSP------FETFVGLNSRDWGTV 107 + G EDLGGG A+F N +DTG +T VGL S ++GTV Sbjct: 57 GLVKGSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGL-SGNFGTV 115 Query: 108 TLGNQYDLLADLVPYTSER-FTSNLATHPGNLDRTVGNPLNSLVKYKSPELNGFQFGAQY 166 +G Q D L D+ S F H LDRT G LN+ ++Y SP NG F Y Sbjct: 116 LVGRQNDPLNDIGGIASAGGFGVPGTVHSKGLDRTDGQRLNNSIRYNSPNFNGLSFSGIY 175 Query: 167 GYGTPTSTTNNGNTMGFSASYSSNPWQF 194 G+G G ++G + Y + P Sbjct: 176 GFGEQVGRAVGGRSIGLGSQYVNGPLSL 203 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 110/394 (27%), Positives = 153/394 (38%), Gaps = 44/394 (11%) Query: 2 LKKTLFAGAVTLVAASAHGQTQNVTISGVLGAGVSYTNNASYPNGG-GRMQQGNTHAV-- 58 +KK+L A AV A A +VTI G++ GV Y + + P GG G N+ + Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQG 60 Query: 59 PFIAFTGREDLGGGAFAIFKWANYVQLDTGGFSPFET---------FVGLNSRDWGTVTL 109 I F G EDLGGG A+F DTG +T VGL + ++G+V L Sbjct: 61 SRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGL-AGNFGSVLL 119 Query: 110 GNQYDLLADLVPYTS-ERFTSNLATHPGNLDRTVGNPLNSLVKYKSPELNGFQFGAQYGY 168 G Q D + YTS + F NLDR G N+ + Y + LNGF YG+ Sbjct: 120 GRQTDFADTISSYTSVQDFGGWTGAAGHNLDRLEGTRTNNSISYTTANLNGFTGNLMYGF 179 Query: 169 GTPTSTTNNGNTMGFSASYSSNPWQFLAVWETVHDVPYSPRSSLGVPTLYGIDLVKNPTT 228 G T+ G G Y + P + +SL T G + N T Sbjct: 180 GETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAAT--GTNQFTNGTN 237 Query: 229 KVAQNQNTASAGVVYSDAGWRLMSNYSYTK--------LSALGV-------------SAK 267 + Q + Y R+ N+S K L+A GV S K Sbjct: 238 NGSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNK 297 Query: 268 AQSIDLGAYKYVTTALRLGGGYGYTKLE------NYKWNMVHAHADYALSKRTSLYALSV 321 A ++G ++ +L+L +T+ + K V DY LSKRT LYA Sbjct: 298 ADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAFLA 357 Query: 322 FQAAGQGQVAAMRLQPIASTNRQVVFEVGITHVF 355 + A + N+ V VGI H F Sbjct: 358 YSKATDLNNPGVADSSGTDGNQGAV-AVGIRHKF 390 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,083,379 Number of extensions: 45625 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 12 Length of query: 355 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 269 Effective length of database: 1,212,660 Effective search space: 326205540 Effective search space used: 326205540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)