BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0136.p (341 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 117 1e-27 gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 112 5e-26 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 89 7e-19 gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase fa... 64 2e-11 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 117 bits (293), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 75/229 (32%), Positives = 115/229 (50%), Gaps = 5/229 (2%) Query: 75 EVPVRVYRPKASQGK-VPTIVFFHGGGFVLGSIELMDDVARKMCRDTGAVVVSVDYRLAP 133 ++ + + RP S K +P +FFHGGG+VLG + + R + ++GAV V V+Y +P Sbjct: 86 QIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVFVNYTPSP 145 Query: 134 ENPFPAAHDDALAATNWVVDNVELLGGDATRVAVAGESAGANLATTAAILLSRERPKSLV 193 E + A + A AAT WV ++ + + D R+AVAG S G N+A A++ + +L Sbjct: 146 EAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPALR 205 Query: 194 AQLLVVPGVDMARDVSEIEDRGIDYPMLKPSDLRDISRLYMGDQASRAKGFPPSPLRAF- 252 +++L+ P D + + Y L + ++ Y D A R K SPL A Sbjct: 206 SEVLLWPVTDANFETASYNQFANGY-FLTKNMMKWFWDSYTTDAAKR-KEITASPLNATP 263 Query: 253 -SLDGAPPALVAVAGHDPLRQEGLAYAEKLSQNRVPVKVLNFDSMFHLF 300 L G PP L+ A D LR EG Y KL+ V V + ++ M H F Sbjct: 264 AQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAVTSVRYNGMIHDF 312 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 112 bits (279), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 77/219 (35%), Positives = 112/219 (51%), Gaps = 5/219 (2%) Query: 92 TIVFFHGGGFVLGSIELMDDVARKMCRDTGAVVVSVDYRLAPENPFPAAHDDALAATNWV 151 +++FHGGG+ +GS++ D + + T V++VDYRLAPE+ FP A +DA A +W+ Sbjct: 86 VLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAVDYRLAPEHRFPTAFEDAADAYDWL 145 Query: 152 VDNVELLGGDATRVAVAGESAGANLATTAAILLSRERPKSLVAQLLVVPGVDMARDVSEI 211 + + LG R+AV G+SAG LA A+ + + Q+L+ P +D Sbjct: 146 LQHGPALGLATNRLAVGGDSAGGTLAAALALRARQAARAPCL-QVLLYPCTSAHQDGDSH 204 Query: 212 EDRGIDYPMLKPSDLRDISRLYMGDQASRAKGFPPSPLRAFSLDGAPPALVAVAGHDPLR 271 Y + + GD + F +PL+A + GA PALV + HDPL Sbjct: 205 RRFATGYLLEGETLQWMFGHTLNGDHERQDWRF--APLQAADVSGAAPALVVIGDHDPLL 262 Query: 272 QEGLAYAEKLSQNRVPVKVLNFDSMFHLF--FGMFEASA 308 EG+AYAEKL Q VPV++ M H F GM A A Sbjct: 263 DEGMAYAEKLRQAGVPVQLDIHPGMVHDFLRLGMVVAEA 301 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 88.6 bits (218), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 87/239 (36%), Positives = 116/239 (48%), Gaps = 16/239 (6%) Query: 68 LVNGQLVEVPVRVYRPK---ASQGKVPTIVFFHGGGFVLGSIELMDDVARKMCRDTGAVV 124 V G L +PVR+Y P A K P +++FHGGGFV GS+E D AR + V Sbjct: 58 FVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAV 117 Query: 125 VSVDYRLAPENPFPAAHDDALAATNWVVDNVELLGGDATRVAVAGESAGANLATTAAILL 184 +SV Y LAP PFPAA +DA AA W N L D R+ VAG+ AG LA + L+ Sbjct: 118 LSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLT-LM 176 Query: 185 SRERP-KSLVAQLLVVPGVD----MARDVSEIEDRGIDYPMLKPSDLRDISRLYMGDQAS 239 +R+R K L AQ+L+ P +D + D + ++ L R Y+ Sbjct: 177 TRDRCGKPLAAQVLISPMLDPSMRLLGDAARLQSD------LTAETCAASYRQYLPQTMQ 230 Query: 240 RAKGFPPSPLRAFSLDGAPPALVAVAGHDPLRQEGLAYAEKLSQNRVPVKVLNFDSMFH 298 R + +PL L G PPA +A A D L E YA L + VP +V F + H Sbjct: 231 RLHPY-AAPLEVSRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFAGITH 288 >gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase family protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 72/229 (31%), Positives = 100/229 (43%), Gaps = 31/229 (13%) Query: 80 VYRPKAS-----QGKVPTIVFFHGGGFVLGSIELMDDVARKMCRDTGAVVVSVDYRLAPE 134 +YRP + QG+ P +VFF+GG + GS + V + G + V DYRL PE Sbjct: 59 IYRPADTASAPPQGR-PVVVFFYGGSWNQGSRKDYAFVGEALA-SRGVIAVLPDYRLYPE 116 Query: 135 NPFPAAHDDALAATNWVVDNVELLGGDATRVAVAGESAGA-NLATTAAILLSRERPKSLV 193 +P D+ A W + +++ LGGD RV V G SAGA N A A + + Sbjct: 117 VSYPDFLQDSAQAVAWTLRSLQALGGDPQRVFVMGHSAGAYNAAMMALDARWLDAAGATP 176 Query: 194 AQLLVVPGVDMARDVSEIEDRGIDYPMLKPSDLRDISRLYMGDQASRAKGFPPSPLRAFS 253 AQL G+ D IE+ P +KP + D ++ P+R Sbjct: 177 AQLRGWIGLAGPYDFLPIEN-----PDVKPV-------FHFPDSPPDSQ-----PVRHVQ 219 Query: 254 LDGAPPALVAVAGHDPL----RQEGLAYAEKLSQNRVPVKVLNFDSMFH 298 APPAL+ + D L R G A L Q+ VPV FD+ H Sbjct: 220 AS-APPALLIASHQDKLVSATRNTG-GLAALLRQHGVPVVETYFDNTSH 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,126,742 Number of extensions: 49653 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 4 Length of query: 341 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 255 Effective length of database: 1,212,660 Effective search space: 309228300 Effective search space used: 309228300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)