BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0135.p (281 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 152 4e-38 gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 82 4e-17 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 152 bits (383), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 85/205 (41%), Positives = 127/205 (61%), Gaps = 7/205 (3%) Query: 69 KIICVGLNYADHTKESNFAQPDYPTLFFRVNTSLVAHAEPMIRPAVKDSEGLDFEGEIAA 128 K IC+GLNYADH ESN P P +F + +++V + + P + S+ D+E E+ Sbjct: 72 KFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIP--RGSKKTDWEVELGV 129 Query: 129 VIGKGGRHIAPADALSHVAGYSLFNDGSVREYQF-KTPQWTVGKNFDATGAFGPDLVTAD 187 +IGKGG +I DA+SHVAGY + ND S REYQ + W GK D G GP LVT D Sbjct: 130 IIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGPIGPWLVTRD 189 Query: 188 ELPAGAK-GLRLETILNGQVVQSASTDEMVFDVATVISIVSQAITLEPGDVIVTGTPSGI 246 E+ K G+ LE ++G+ Q+ +T M+F+VA ++S +S+ ++L+PGDVI TGTP G+ Sbjct: 190 EVADPQKLGMWLE--VDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVISTGTPPGV 247 Query: 247 GWARSPK-LLMKAGDVCEVRVEGIG 270 G P+ + ++AG + ++G+G Sbjct: 248 GMGVKPEAVYLRAGQTIRLGIDGLG 272 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 82.4 bits (202), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 59/185 (31%), Positives = 88/185 (47%), Gaps = 11/185 (5%) Query: 69 KIICVGLNYADHTKESNFAQPDYPTLFFRVNTSLVAHAEPMIRPAVKDSEGLDFEGEIAA 128 +I CVG NYA H +E P FF V + ++ V S+ E E+ Sbjct: 25 RIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSVVPYPVATSD-YHHEIELVV 83 Query: 129 VIGKGGRHIAPADALSHVAGYSLFNDGSVREYQFKTPQ----WTVGKNFDATGAFGP--D 182 + KGGR+I AL V GY++ D + R+ Q + + W + K FD + P Sbjct: 84 ALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWDMAKGFDQSAPCAPIVP 143 Query: 183 LVTADELPAGAKGLRLETILNGQVVQSASTDEMVFDVATVISIVSQAITLEPGDVIVTGT 242 + A GA L++ NG+V Q ++++ VA IS +S + L PGD+I TGT Sbjct: 144 VAQAGHPDQGAVWLKV----NGEVRQQGDLADLIWSVAETISYLSGLVELFPGDLIFTGT 199 Query: 243 PSGIG 247 P G+G Sbjct: 200 PEGVG 204 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 940,477 Number of extensions: 41601 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 2 Length of query: 281 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 197 Effective length of database: 1,222,130 Effective search space: 240759610 Effective search space used: 240759610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)