BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0132.p (158 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309972|ref|YP_003774064.1| hypothetical protein Hsero_0637... 211 2e-56 >gi|300309972|ref|YP_003774064.1| hypothetical protein Hsero_0637 [Herbaspirillum seropedicae SmR1] Length = 147 Score = 211 bits (537), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 95/136 (69%), Positives = 107/136 (78%) Query: 22 ILQTKFMHVKPGDTQYVEGGLRDFFVYRDLGIAEATHGKVIAQLVRANAAPEAGTGWHVH 81 + Q F HV GDT Y GGLRDFF+YRDLGIA AT G+V+AQLV+AN AP+AGTGWH H Sbjct: 10 LAQNTFSHVSEGDTPYETGGLRDFFLYRDLGIAAATGGQVVAQLVKANTAPQAGTGWHRH 69 Query: 82 AAEFHIVYMLKGWAKFMYEDQETLVSAGDCVHQAPGIRHFLFDYSPDMEYLEVVGPADFA 141 A FHIV MLKGWA+FMYE + TLV AGDCVHQ PGI HFLFDYSPDMEYLE+V PADF Sbjct: 70 EANFHIVIMLKGWARFMYETEVTLVKAGDCVHQRPGIVHFLFDYSPDMEYLEIVSPADFK 129 Query: 142 SVGVEGPCTVPQPAPW 157 ++ + PC VP P PW Sbjct: 130 TIAMPAPCEVPAPTPW 145 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 529,196 Number of extensions: 21294 Number of successful extensions: 53 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 1 Length of query: 158 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 80 Effective length of database: 1,250,540 Effective search space: 100043200 Effective search space used: 100043200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)