BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0130.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 167 1e-42 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 110 1e-25 gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 82 9e-17 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 167 bits (424), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 107/334 (32%), Positives = 172/334 (51%), Gaps = 22/334 (6%) Query: 37 RQGYPIQLFEQAQEIKEIGAGIQIGPNAFRVFERLGLVEKVTAKASMPESLVMMDGITAE 96 ++G+ ++++EQA +GAGI +GPN +V R+G+ +++ + S P+ T + Sbjct: 25 QEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGD 84 Query: 97 EVVSIPLQGQFRERFQYPYALIHRADLHQIFVDACAAELNIEVVTGTRVENFTQEGALVT 156 + IPL + + Y +HR D H++ + A +E + G + G +V Sbjct: 85 VLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPSE---RLSFGKCLTKVEDRGDVVI 141 Query: 157 LHTERGDYTGAAL-IGADGLWSTIRAQIANDGPPRVSGHIAYRAVLPTSDVPE-ELRSNT 214 L G A + IGADG+ S IR ++ PP+ +G++A+RAV PT ++ L + Sbjct: 142 LSFADGTTEEADIVIGADGVNSRIREELLGVEPPKYAGYLAHRAVFPTPELKAGMLPFDA 201 Query: 215 MTLWAGAKTHLVQYHL--RGGDLFNLVAVFHSDKYVEGWDSK-----GDKEELHKRFCDA 267 W H++ Y + + +L+ + V VE WD KEE+ + F Sbjct: 202 CVKWWSDDRHMMVYFVTSKANELYYVTGV-----PVEKWDLNDRWLPSSKEEMRETFHGW 256 Query: 268 APPVKALLDKIDSWRMWVLCDREPIKQWTSGNVVLLGDAAHPMLQYMAQGACMAMEDAVV 327 P V+AL+D W L +R+P+ W+ G +VLLGDA HPM +MAQGA MA+ED + Sbjct: 257 HPTVQALVDATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMKPHMAQGAAMAIEDGAM 316 Query: 328 LA---EEVGRDATDFASAFVRYQNRRYLRTGKVQ 358 LA +EVG +++A AF Y+ R R KVQ Sbjct: 317 LARCFKEVG--VSNYADAFALYEANRAARASKVQ 348 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 110 bits (276), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 113/385 (29%), Positives = 176/385 (45%), Gaps = 44/385 (11%) Query: 19 ILVAGGGIGGFAAALCLARQGYPIQLFEQAQEIKEIGAGIQIGPNAFRVFERLGLVEKVT 78 IL+ GGG G +AA+ L R+G + L E + + GAGI +GP R ++LG++E Sbjct: 8 ILIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAFL 67 Query: 79 AKASMPESLVMMDGITAEEVVSIPLQGQFRERFQYPY----ALIHRADLHQIFVDACAAE 134 + + + V + +V +P R P I R L +I DA A Sbjct: 68 REGAAGDG-VRLCLPHGPQVAELPT-----PRLASPDVPGGGAILRPVLARILADATRAA 121 Query: 135 LNIEVVTGTRVENFTQEGALVTL-----HTERGDYTGAALIGADGLWSTIRAQIANDGP- 188 +V G + G V + T R D +IGADGL+S +R + P Sbjct: 122 -GADVRLGCTFSAVREVGHKVEVDFTDGQTRRYDL----VIGADGLYSKLRMHLFPHAPK 176 Query: 189 PRVSGHIAYRAVLPTSDVPEELRSNTMTLWAGAKTHLVQYHLRGGDLFNLVAVF-----H 243 PR SG +RAVLP P+E+ T T+W G + + +++ V H Sbjct: 177 PRYSGQAVWRAVLPR---PQEI--VTCTMWMGPRIKPGVNPVSKDEMYLFVTEPRPVNEH 231 Query: 244 SDKYVEGWDSKGDKEELHKRFCDAAPPVKALLDKIDSWRMWVLCDREPI---KQWTSGNV 300 D +G EE +AP +K + +++D + E + + W G V Sbjct: 232 VDPTTFVSHLRGLLEEF------SAPVLKTIREQLDDNARIIFRPLEGLLLPRPWYQGRV 285 Query: 301 VLLGDAAHPMLQYMAQGACMAMEDAVVLAEEVGRDATDFASAFVRYQNRRYLRTGK-VQM 359 VL+GDA H ++A GAC+ +EDA+VLA+E+ R T A ++ RR+ R V+ Sbjct: 286 VLIGDAVHATTPHLASGACIGIEDALVLADELERHGT-VPQALAAFEERRWERCRMVVEN 344 Query: 360 SARLYGEFYHAAGVNRELRNQFLRA 384 SARL GE G +++ ++ +RA Sbjct: 345 SARL-GEI-EVEGGDKDEHSRIMRA 367 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 81.6 bits (200), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 92/375 (24%), Positives = 149/375 (39%), Gaps = 38/375 (10%) Query: 19 ILVAGGGIGGFAAALCLARQGYPIQLFEQAQEIKEIGAGIQIGPNAFRVFERLGLVEKVT 78 + +AG G G A AL L R G+ + L E+ + +G+G+ + P V + LGL ++ Sbjct: 6 VAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGLFSRIA 65 Query: 79 AKASMPESLVMMDGITAEEVVSIPLQGQFRERFQYPYALIHRADLHQIFVDACAAELNIE 138 A + L D ++ + V+ + R+ +HRA L + ++A E IE Sbjct: 66 ALGQRIDRLYGADALSGKTVLDVRYDAIAGGRYGI---AVHRAALFGVLLEAAQQE-GIE 121 Query: 139 VVTGTRVENFTQEGALVTLHTERGDYTGA--ALIGADGLWSTIRAQIANDGPPRVSGHIA 196 V TG + N GA L G G +I A G S +R + PR Sbjct: 122 VETGQDIGNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPR------ 175 Query: 197 YRAVLPTSDVPEELRSNTMTLWAGAKTHLVQYHLRGGDLFNLVAVFHSDKYVEG-----W 251 LP LR+ + LVQ + R + ++ + E W Sbjct: 176 ---PLPYGAFWVSLRAQPGLSF--GHNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFW 230 Query: 252 DSKGDKEELHKRFCDAA---------PPVKALLDKIDSWRMWVLC--DREPIKQWTSGNV 300 ++ D+ E K+ +A P L +ID + L + + Sbjct: 231 STRPDRVESLKQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYGERL 290 Query: 301 VLLGDAAHPMLQYMAQGACMAMEDAVVLAEEVGRDATDFASAFVRY--QNRRYLRTGKVQ 358 ++GDAAH + QGA MA+ DA L+ + + T A RY RR++RT Q Sbjct: 291 AIIGDAAHSTSPQLGQGANMALLDARALSHALQQSET-LEQALPRYAASRRRHVRT--FQ 347 Query: 359 MSARLYGEFYHAAGV 373 + ++ FY + V Sbjct: 348 AMSAMFTPFYQSDSV 362 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,213,402 Number of extensions: 50033 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 3 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)