BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0127.p (444 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 299 3e-82 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 223 2e-59 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 205 6e-54 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 111 1e-25 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 103 3e-23 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 299 bits (765), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 164/436 (37%), Positives = 269/436 (61%), Gaps = 6/436 (1%) Query: 6 SLYVNEVIDSRPVGGLQWCSIVTCILIAVLDGFDTQTIGMLAPAMSKDLGIPISQFGPIF 65 +++V++V++ G Q+ ++ C L ++DGFD Q +G +APA+ D G+ + GP+F Sbjct: 11 TVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVF 70 Query: 66 SAGLLGMLIGAVVLGPMADRFGRKTMVVLSTIIFGVLSFATAYASGHDELLVLRFLTGIG 125 AGL GML+G++VL P+ DR+GR+ +++LST+ F + AT + D+LLVLRF+TG G Sbjct: 71 GAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFG 130 Query: 126 LGGALPNALALASEYSPR--RYSRVIVSTLMCGMPLGAVLGGIVASILLPLHGWHSVFMV 183 LG +PNA+AL E+SP R +R+++ + CG +GA GG V++ L+P +GWH+VF V Sbjct: 131 LGSIMPNAMALVGEFSPSSSRVTRMMLVS--CGFTVGAAAGGFVSAALIPAYGWHAVFWV 188 Query: 184 GGVLPVAVALFAIAFMPESARFLILRNQSDRRLGAIMHRIAPDLPREQQFHVPL--SRST 241 GG +P+ + L + ++PES +FL+L + ++ + ++ P++ + + V + +++ Sbjct: 189 GGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAE 248 Query: 242 GVPIGELFSKGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLVGAHHPVSVGIAAISFFSG 301 G+P+ LF GR TVLLW+ FMNL+ LYF+ +W+P ++ A + S+ + + Sbjct: 249 GMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQV 308 Query: 302 GGVVGCLLQGWLMNSFGVRKVLVTELVLYAVLAAVLANWPSSYATVVVVSTLMGVVIQGA 361 GGV+G L G + FG +VL + +L + A++ + A + V + G I G Sbjct: 309 GGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVGG 368 Query: 362 QAGFNVLATEIYPTHMRATGVGFAIGIGRIGSICGPLSGGLMLALQLDVKHIFLMGIAPA 421 Q N LA YPT +R+TG+G+A+GIGRIGS+ GP+ GG ++ALQ +FL PA Sbjct: 369 QPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAVPA 428 Query: 422 LLASLAILLNRRADQG 437 L+++L I R QG Sbjct: 429 LISALTIARLHRTAQG 444 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 223 bits (569), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 135/406 (33%), Positives = 225/406 (55%), Gaps = 6/406 (1%) Query: 4 SSSLYVNEVIDSRPVGGLQWCSIVTCILIAVLDGFDTQTIGMLAPAMSKDLGIPISQFGP 63 + ++ + ++D G Q + L V+DGFD Q IG P++ K+ GI + F P Sbjct: 9 NQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAP 68 Query: 64 IFSAGLLGMLIGAVVLGPMADRFGRKTMVVLSTIIFGVLSFATAYASGHDELLVLRFLTG 123 +AGL+GM IG+ G ADRFGR+ ++ S +FGV + A++ + +LRF G Sbjct: 69 AVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAG 128 Query: 124 IGLGGALPNALALASEYSP-RRYSRVIVSTLMCGMPLGAVLGGIVASILLPLHGWHSVFM 182 +G+GGALP++ + +E+SP RR + + +T++C +PLG +L G+ A+ +LP +GW +F+ Sbjct: 129 LGIGGALPSSTTMTAEFSPARRRTLAVTATIVC-VPLGGMLAGLFANAVLPHYGWRGLFL 187 Query: 183 VGGVLPVAVALFAIAFMPESARFLILRNQSDRRLGAIMHRIAPDLPREQQFHVPLSRS-- 240 +GG+LPVA ++ +PES R+L L ++ R++ + + F ++ Sbjct: 188 IGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIE 247 Query: 241 TGVPIGELFSKGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLVGAHHPVSVGIAAISFFS 300 T G LF+ G+ +DT+ LW+ +FMNL+ +Y SW+P +L G SV A ++ ++ Sbjct: 248 TRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYN 307 Query: 301 GGGVVGCLLQGWLMNSFGVRKVLVTELVLYAVLAAVLANWP-SSYATVVVVST-LMGVVI 358 GGV G LL + FG R ++ + A L + +S T++VV L G+ + Sbjct: 308 FGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLHGLFV 367 Query: 359 QGAQAGFNVLATEIYPTHMRATGVGFAIGIGRIGSICGPLSGGLML 404 Q+ L IYPT +RATG A+ GR+G+I +G L++ Sbjct: 368 NAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVI 413 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 205 bits (522), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 139/427 (32%), Positives = 232/427 (54%), Gaps = 7/427 (1%) Query: 5 SSLYVNEVIDSRPVGGLQWCSIVTCILIAVLDGFDTQTIGMLAPAMSKDLGIPISQFGPI 64 +L V+++ID W + C L+ V DG+D G + P + K+ + Q G + Sbjct: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 Query: 65 FSAGLLGMLIGAVVLGPMADRFGRKTMVVLSTIIFGVLSFATAYASGHDELLVLRFLTGI 124 S L GM+ GA + GP+ADR GRK V +S ++F V +F + +A+ E V RFL G+ Sbjct: 62 GSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGL 121 Query: 125 GLGGALPNALALASEYSPRRYSRVIVSTLMCGMPLGAVLGGIVASILLPLHGWHSVFMVG 184 G GG +PNA+AL +EY+P+R +V+ + G LG VL + +LP GW S+F Sbjct: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAA 181 Query: 185 GVLPVAVALFAIAFMPESARFLILRNQSDRRLGAIMHRIAPD--LPREQQFHVPLSRSTG 242 V P+ + + +PES FL LR + A++ ++ P + + Q + G Sbjct: 182 AV-PLVLLPVILWKLPESVGFL-LRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAG 239 Query: 243 VPIGELFSKGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLVGAHHPVSVGIAAISFFSGG 302 P+ LF + R T+++W+ +F L+++Y + SW+P ++ A + + ++ + + G Sbjct: 240 APMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFG 299 Query: 303 GVVGCLLQGWLMNSFGVRKVLVTELVLYAVLAAVLA-NWPSSYATVVVVSTLMGVVIQGA 361 GVVG + GWL + F + KV+V+ + V A+L N P+ ++++ + G G Sbjct: 300 GVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLII--VAGATTIGT 357 Query: 362 QAGFNVLATEIYPTHMRATGVGFAIGIGRIGSICGPLSGGLMLALQLDVKHIFLMGIAPA 421 Q + YP +R+TG+G+A G+GR G+I GPL GG ++A L +K FL+ P Sbjct: 358 QILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPG 417 Query: 422 LLASLAI 428 L+A+LA+ Sbjct: 418 LVAALAM 424 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 107/374 (28%), Positives = 173/374 (46%), Gaps = 29/374 (7%) Query: 34 VLDGFDTQTIGMLAPAMSKDLGIPISQFGPIFSAGLLGMLIGAVVLGPMADRFGRKTMVV 93 + D F ++ + P + + +Q G + ++G LG +IGA+ G MA+R+GR Sbjct: 54 LFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSAS 113 Query: 94 LSTIIFGVLSFATAYASGHDELLVLRFLTGIGLGGALPNALALASEYSPR--RYSRVIVS 151 ++ + VLS A L + RFL GIG+GG +P A +E S R ++ Sbjct: 114 VTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLY 173 Query: 152 TLMCGMPLGAVLGGIVASILLPLHGWHSVFMVGGVLPVAVALFAIAFMPESARFLILRNQ 211 L+ PLG + V + L+P GW +F+VGG+ + VAL I +PES R+LI R + Sbjct: 174 ELI--FPLGLLAAAQVGAFLVPRFGWEYMFLVGGIPGILVALL-ITRLPESPRWLIARGR 230 Query: 212 SD------RRLGAIMHRIAPDLPREQQFHVPLSRSTGVPIGELFSKGRWKD--------- 256 D R L A + D ++Q + G+ +G WK+ Sbjct: 231 LDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKR-QRGSWKELFSPVYRGR 289 Query: 257 TVLLWIPYFMNLVVLYFIVSWMPAVLVGAHH---PVSVGIAAIS-FFSGGGVVGCLLQGW 312 T+++W+ + + V I +W+P++ +H S+ +A++S S V+GC L Sbjct: 290 TIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCAL--- 346 Query: 313 LMNSFGVRKVLVTELVLYAVLAAVLANWPSSYATVVVVSTLMGVVIQGAQAGFNVLAT-E 371 L++ G R+ + +L L A+L + A V+ + G L T E Sbjct: 347 LIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAVMGTTTVLLYLYTPE 406 Query: 372 IYPTHMRATGVGFA 385 IYPT MRA G G A Sbjct: 407 IYPTRMRAIGTGLA 420 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 103 bits (256), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 106/398 (26%), Positives = 181/398 (45%), Gaps = 18/398 (4%) Query: 9 VNEVIDSRPVGGLQWCSIVTCILIAVLDGFDTQTIGMLAPAMSKDLGIPISQFGPIFSAG 68 V + + + VG Q+ V L+ + D +IG AP ++K G+ + Q + Sbjct: 8 VEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMF 67 Query: 69 LLGMLIGAVVLGPMADRFGRKTMVVLSTIIFGVLSFATAYASGHDELLVLRFLTGIGLGG 128 +GML+GA V G +ADR GR+ +++ + +I A+A+A LL LR LTGIG+GG Sbjct: 68 FVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGG 127 Query: 129 ALPNALALASEYSPR-RYSRVIVSTLMCGMPLGAVLGGIVASILLPL--HGWHSVFMVGG 185 LP + +E+ P R R +V L +G + ++A + + W +F V G Sbjct: 128 TLPVDYTMMAEFLPSARRGRWLV-MLESFWAIGTICLAVLALVAVSWGNDAWRVIFFVTG 186 Query: 186 VLPVAVALFAIAFMPESARFLILRNQSD------RRLGAIMHRIAPDLPREQQFHVPLSR 239 LP V + ++PES +L +SD R+ + P P + + + R Sbjct: 187 -LPALVGVVLRFYVPESPMYLNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEV---VER 242 Query: 240 STGVPIGELFSKGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLVGAHHPVSVGIAAISFF 299 P+ LFS + ++ L++ + + + Y + ++P L G + F Sbjct: 243 K---PMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFL 299 Query: 300 SGGGVVGCLLQGWLMNSFGVRKVLVTELVLYAVLAAVLANWPSSYATVVVVSTLMGVVIQ 359 + + G L + + +G + LV LVL AV +L + VV + L+ + Sbjct: 300 ALVQLPGYALSAYGVERWGRKPTLVGFLVLSAV-GCMLYSLGKDPMLVVGSTLLLSFSLL 358 Query: 360 GAQAGFNVLATEIYPTHMRATGVGFAIGIGRIGSICGP 397 G A E+YPT +RA+G+G A + R G + P Sbjct: 359 GTWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAP 396 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,251,101 Number of extensions: 51796 Number of successful extensions: 437 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 401 Number of HSP's successfully gapped: 36 Length of query: 444 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 356 Effective length of database: 1,203,190 Effective search space: 428335640 Effective search space used: 428335640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)