BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0126.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 590 e-170 gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 109 4e-25 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 101 2e-22 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 590 bits (1521), Expect = e-170, Method: Compositional matrix adjust. Identities = 287/462 (62%), Positives = 352/462 (76%) Query: 11 TLFEKLWQAHVVSSDDEGNALVYIDRQYVYEVTSPQAFEGLREKKRKVRRPGTVLATVDH 70 TL++KLW++HVV ++D+G A++YIDR ++EVTSPQAFEGL+ R+ R L DH Sbjct: 4 TLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMVADH 63 Query: 71 NVPTTARDVPMRDRLAQLQVDTLTSNCADFGIPYFDIRDVRQGIVHVVGPEQGATLPGST 130 NVPTT R + D +++LQV+TL N ++ + YF + D RQGIVHV+GPEQGATLPG T Sbjct: 64 NVPTTDRAHGIADPISRLQVETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLPGMT 123 Query: 131 VICGDSHTSTHGAFGALAWGVGTTEVEHALATHCLWLVRPLTTRITVKGTLAPGVGAKDL 190 V+CGDSHTSTHGAF ALA G+GT+EVEH LAT L + + V G L GV AKD+ Sbjct: 124 VVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTAKDI 183 Query: 191 ALYLIGFLGTQGGIGHAIEFAGDAIAALSMEGRMTLCNMAVEAGARFGLVAVDDTTIDYL 250 L +IG +GT GG G+AIEFAG I +LSMEGRMT+CNMA+EAGAR G+VA DDTT++YL Sbjct: 184 VLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEYL 243 Query: 251 QGRPLVPSGAQWDAANAYWRQLRSDSGAQFYRELEIDAGTVKPMVTWGTSPDMCVGVDGV 310 +GRP PSG W+ A +YWR L SD GA+F + ++A +KP VTWGTSP+M V VD Sbjct: 244 KGRPFSPSGPHWERAVSYWRSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAVDAR 303 Query: 311 VPDPSQEENITRQSGMRKALAYMDISAGTPMEQLALDKVFIGSCTNARIEDLRAAAAVVR 370 VPDP +E++ T++ GM KALAYM + TP+E + +DKVFIGSCTN+RIEDLR AA VVR Sbjct: 304 VPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAKVVR 363 Query: 371 GYTVSQTIRQALVVPGSGLVKRQAEQEGLDKVFLAAGFEWREPGCSMCLGMNDDRLAPGE 430 G + ++ A+VVPGSGLVK QAE+EGLDK+F AGFEWREPGCSMCL MN DRL PGE Sbjct: 364 GKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPGE 423 Query: 431 RCASTSNRNFEGRQGPGGRSHLVSPATAAAAAICGHFTDVRT 472 RCASTSNRNFEGRQG GGR+HLVSPA AAAA I GHF DVR+ Sbjct: 424 RCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRS 465 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 109 bits (273), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 127/443 (28%), Positives = 187/443 (42%), Gaps = 52/443 (11%) Query: 68 VDHNVPTTARDVPMRDRLAQLQVDTLTSNCADFGIPYFDIRDVRQ---GIVHVVGPE--- 121 +DH A D+ M+ ++ + +G+ FD V GIVH V E Sbjct: 134 IDHFREKKALDLNMKLEFSR---NNERYQFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLA 190 Query: 122 ------QGATLPGSTVICGDSHTSTHGAFGALAWGVGTTEVEHALATHCLWLVRPLTTRI 175 G P T++ DSHT+ G + WGVG E E + ++ + P + Sbjct: 191 RGVHNKTGVYYP-DTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGV 249 Query: 176 TVKGTLAPGVGAKDLALYLIGFLGTQGGIGHAIEFAGDAIAALSMEGRMTLCNMAVEAGA 235 + G L GV A DL L + L +G +EF G+ +LS+ R T+ NMA E GA Sbjct: 250 NLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATIANMAPEYGA 309 Query: 236 RFGLVAVDDTTIDYLQGRPLVPSGAQWDAANAYWR--QLRSDSGA---QFYRELEIDAGT 290 G VDD TI+Y +G + A+ DA AY++ +L A + +E+ +D GT Sbjct: 310 TMGFFPVDDATIEYFKGTGR--TKAEIDAFEAYFKAQELYGVPKAGQIDYTQEVSLDLGT 367 Query: 291 VKPMVTWGTSPDMCVGVDGV-------VPDPSQEENITRQSGMRKALAYMDISAGTPMEQ 343 V P + P + + V P+ E +++ A +Y + + G + Sbjct: 368 VAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGA-SYKN-ADGVDLHN 425 Query: 344 LALDKVFIGSCTNARIEDLRAAA------AVVRGYTVSQTIRQALVVPGSGLVKRQAEQE 397 + I SCTN + AA AV G V+ I+ +L PGS +V + E Sbjct: 426 GDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSL-APGSRVVTKYLEAA 484 Query: 398 GLDKVFLAAGFEWREPGCSMCLGMNDDRLAPGERCAS-----------TSNRNFEGRQGP 446 GL GF GC+ C+G N L P A + NRNFE R P Sbjct: 485 GLLPYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEARIHP 543 Query: 447 GGRSH-LVSPATAAAAAICGHFT 468 R++ L SP A AI G+ T Sbjct: 544 NIRANFLASPPLVVAYAIAGNVT 566 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 101 bits (251), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 108/385 (28%), Positives = 160/385 (41%), Gaps = 51/385 (13%) Query: 112 QGIVHVVGPEQ---------GATLPGSTVICGDSHTSTHGAFGALAWGVGTTEVEHALAT 162 GI+H + E+ G P T++ DSHT A G +A GVG E E+ + Sbjct: 169 NGIMHQINLEKMSPVIHAKDGVAFP-DTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLG 227 Query: 163 HCLWLVRPLTTRITVKGTLAPGVGAKDLALYLIGFLGTQGGIGHAIEFAGDAIAALSMEG 222 W+ P + + G PG+ A D+ L L FL Q +G +EF GD A+L++ Sbjct: 228 RASWMRLPDIIGVELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGD 287 Query: 223 RMTLCNMAVEAGARFGLVAVDDTTIDYLQ--GRPLVPSGAQWDAANAYWRQ--LRSDSGA 278 R T+ NMA E GA + ++D TIDYL+ GR Q Y ++ L SD+ A Sbjct: 288 RATISNMAPEYGATAAMFSIDQQTIDYLKLTGR----EDEQVKLVETYAKEAGLWSDTLA 343 Query: 279 --QFYRELEIDAGTVKPMVTWGTSPDMCVGVDGVVPDPSQEENITRQSGMRKALAYMDIS 336 ++ R L D TV + ++P + V + + +A + Sbjct: 344 KVEYERVLRFDLSTVVRTLAGPSNPHKRLPVSELAA---------------QGIAGKVEN 388 Query: 337 AGTPMEQLALDKVFIGSCTN-ARIEDLRAAAAVVRGYTVSQTIRQALV----VPGSGLVK 391 M A+ I SCTN + ++ AA + R R+ V PGS V+ Sbjct: 389 EPGKMPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLARKPWVKSSLAPGSKAVE 448 Query: 392 RQAEQEGLDKVFLAAGFEWREPGCSMCLGMN---DDRLAPG-------ERCASTSNRNFE 441 E+ GL GF C+ C GM+ D ++ + NRNF+ Sbjct: 449 LYLEEAGLTADLEKLGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYSTAVLSGNRNFD 508 Query: 442 GRQGPGGR-SHLVSPATAAAAAICG 465 GR P + + L SP A AI G Sbjct: 509 GRIHPYAKQAFLASPPLVVAYAIAG 533 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,639,829 Number of extensions: 72976 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 3 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)