BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0124.p (365 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 496 e-141 gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspir... 306 1e-84 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 146 3e-36 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 138 8e-34 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 137 2e-33 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 496 bits (1276), Expect = e-141, Method: Compositional matrix adjust. Identities = 246/365 (67%), Positives = 302/365 (82%) Query: 1 MNKKLTALIACATVMAASGQAHAQDAVSIYGIIDTGVLYTTNANANGNGVFKMPSLTGTF 60 M +K++ + A AQ +V+IYGIIDTGV+YTTNANANGN VFKMPSLTG+F Sbjct: 1 MTRKISLHAVALAAALCASSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSF 60 Query: 61 PSRLGFKDAEDLGGGNQALFVLEAGFGPDSGVMGQGGRLFGRQSWVGLKGNWGQVSLGRQ 120 PSR+GFK EDLGGG QA+FVLE+GF PD+G MGQGGRLFGRQS+VGLK +WGQ+ LGRQ Sbjct: 61 PSRIGFKGTEDLGGGLQAMFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQ 120 Query: 121 YAMTYIAMQKTDVMGPNIISISSLDAYLPNARTDNAIGYIGTFEGITLGATYSFGRDASA 180 MTY++ KTDVMGPN+ +I S+DAYLPNAR+DNAIGY+G F G T+GATYSFGRDA+ Sbjct: 121 VNMTYLSQLKTDVMGPNLFAIGSIDAYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAG 180 Query: 181 AGGPAATNCAGEVAGDAKACRQLTGLLGYERREFGINTSYDVLRGGSGAAGGLSNSDAKD 240 GPAATNC GEVAG++KACRQ+T LLGY+ + +G+ +SYD+L G +GA+ G+++SD D Sbjct: 181 PAGPAATNCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTD 240 Query: 241 QRVGLNGYFFVGDLKFGLGLLNRRTDAVANVHTRSNLWYLGVSYPLSAALILDAQVSRLD 300 QRV LNGYF +GD+K G G+++RRT A AN +T S+L+YLGVSYPLS AL+LDAQVSRLD Sbjct: 241 QRVTLNGYFMLGDVKIGGGVVDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLD 300 Query: 301 VKDSPNDVDLAVARLTYMLSKRTALYTSLGYLRNHGTSAIAIDSGGTVKAGSNQTGVMMG 360 VK+SPNDV L+VARLTY LSKRTA+Y SLGY++N GTS++A+D+GGTV AG NQ GVM G Sbjct: 301 VKNSPNDVTLSVARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTG 360 Query: 361 IRHTF 365 IRHTF Sbjct: 361 IRHTF 365 >gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 369 Score = 306 bits (785), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 174/372 (46%), Positives = 236/372 (63%), Gaps = 13/372 (3%) Query: 3 KKLTALIACATVMAASGQAHAQDAVSIYGIIDTGVLYTTNAN---ANGNGVFKMPSLTGT 59 K L A+IA M+A+ + AQ +V++YGIIDTGV Y +N + A G+ ++P+LT T Sbjct: 2 KALKAIIAALLTMSAT-LSQAQ-SVTLYGIIDTGVEYVSNVSNVGAGQQGLLRVPNLTAT 59 Query: 60 FPSRLGFKDAEDLGGGNQALFVLEAGFGPDSGVMGQGGRLFGRQSWVGLKGNWGQVSLGR 119 PSR+G + +EDLGGG +++F+LE+GF PDSG + QGGR+FGR ++VGL +WGQVS GR Sbjct: 60 TPSRIGLRGSEDLGGGLRSVFMLESGFAPDSGALNQGGRMFGRIAYVGLSDHWGQVSFGR 119 Query: 120 QYAMTYIAMQKTDVMGPNIISISSLDAYLPNARTDNAIGYIGTFEGITLGATYSFGRDAS 179 Q M A +D++G N+ ++LD Y PNAR DN+I Y G+F TLG TYSFGRD Sbjct: 120 QATMLLWATLDSDILGANVYGANALDNYRPNARADNSIAYRGSFGAFTLGGTYSFGRDTV 179 Query: 180 AAG-GPAATNCAGEVAGDAKACRQLTGLLGYERREFGINTSYDVLRGGSGAAGGLSNSDA 238 AG PA TNCAGE AGD+ ACR+ + ++ Y+ +G + +YD LRGG GAA GLS+S Sbjct: 180 NAGQNPAGTNCAGEQAGDSLACREWSLMVKYDTAAWGASLAYDTLRGGKGAAAGLSSSQL 239 Query: 239 KDQRVGLNGYFFVGDLKFGLGLLNRRTDAVANVHTRSNLWYLGVSYPLSAALILDAQVSR 298 D R+ L Y LK G+G R A ++S L+Y G+SY L+ L L Q+ Sbjct: 240 SDSRMLLGAYIKADRLKVGIGGSLRHNQGSA--LSKSQLYYAGLSYDLTPTLNLAGQLYY 297 Query: 299 LDVKDSPNDVDLAVARLTYMLSKRTALYTSLGYLRNHGTSAIAIDSGGTV-----KAGSN 353 L DS N L R TY LS RTALY + G++ N G S +++ +GG+V G N Sbjct: 298 LHYDDSANKAWLGALRATYSLSARTALYLTGGFIDNRGQSNLSVSAGGSVGTANPAPGGN 357 Query: 354 QTGVMMGIRHTF 365 Q G+M GIRH+F Sbjct: 358 QFGMMAGIRHSF 369 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 146 bits (368), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 121/360 (33%), Positives = 171/360 (47%), Gaps = 35/360 (9%) Query: 19 GQAHAQDAVSIYGIIDTGVLYTTNANANGNGVFKMPSLTGTFPSRLGFKDAEDLGGGNQA 78 G AQ +V+IYG++DTGV Y NA NG G + T SR+GF+ EDLGGG +A Sbjct: 17 GSVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKA 76 Query: 79 LFVLEAGFGPDSGVM-GQGGRLFGRQSWVGLKGNWGQVSLGRQYAMTYIAMQKTDVMGPN 137 LF LE GF D+G G +LF RQS VGL+G +G+V GR Y+ TY + D MG + Sbjct: 77 LFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYS 136 Query: 138 IISISSLDAYLPNARTD-------NAIGYIGTFEGITLGATYSFGRDASAAGGPAATNCA 190 + A R D N + Y G F G+ +GA Y F Sbjct: 137 ANYSWATTAGATGNRKDGMPTGVSNLVKYQGEFSGVKIGAMYGF---------------- 180 Query: 191 GEVAGDAKACRQLTGLLGYERREFGINTSYDVLRGGSGAAGGLSNSDAKDQRVGLNGYFF 250 GEVAG+ + + LG+ F + +YD R S A A + GY Sbjct: 181 GEVAGETRNGAKYGVGLGWGTGPFSVVATYD--RNNSATATNTPYDRATSAHLAA-GYQP 237 Query: 251 VGDLKFGLGL-LNRRTDAVANVHTRSNLWYLGVSYPLSAALILDAQVSRLDVKDSPNDVD 309 KF LG +++ A RS+ ++ G +Y + A+ L + ++K+ ND D Sbjct: 238 SEAWKFNLGYRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDAD 297 Query: 310 --LAVARLTYMLSKRTALYTSLGY--LRNHGTSAIAIDSGGTVKAGSNQTGVMMGIRHTF 365 + RL Y +SKRT LY S Y +N ++ D G GS+Q G+ +G++H F Sbjct: 298 PIMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAGY---GSSQFGMTLGMQHRF 354 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 138 bits (347), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 120/379 (31%), Positives = 171/379 (45%), Gaps = 52/379 (13%) Query: 8 LIACATVMAASGQAHAQDAVSIYGIIDTGVLYTTNANANGNGVFKMPSLTGTFP--SRLG 65 L+ A + + AHAQ +VSIYG+ID G+ Y ++A +G G ++ + SR+G Sbjct: 5 LLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQGSRIG 64 Query: 66 FKDAEDLGGGNQALFVLEAGFGPDSGVMGQGGRLFGRQSWVGL-KGNWGQVSLGRQYA-M 123 FK EDLGGG A F LE GFG D G +GQGG LFGRQS+VGL WG +S+GRQY M Sbjct: 65 FKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFM 124 Query: 124 TYI-------AMQKTDVMGPNIISISSLDAYLPNA-----RTDNAIGY-IGTFEGITLGA 170 I A+ G + + ++ L N RT+N+I Y F G + GA Sbjct: 125 ANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGA 184 Query: 171 TYSFGRDASAAGGPAATNCAGEVAGDAKACRQLTGLLGYERREFGINTSYDVLRGGSGAA 230 GE AG+ A R ++ Y+ F + Sbjct: 185 MVGL----------------GETAGNNNAGRTVSATGQYDNGPF---------------S 213 Query: 231 GGLSNSDAKDQRVGLNGYFFVGDLKFGLGLLNR--RTDAVANVHT--RSNLWYLGVSYPL 286 G L+ +D KD + + G ++ +G V N +T ++ + LG Y L Sbjct: 214 GALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPFGVVTQVKNTNTSAKATTYELGTVYAL 273 Query: 287 SAALILDAQVSRLDVKDSPNDVDLAVARLTYMLSKRTALYTSLGYLRNHGTSAIAIDSGG 346 + L A + VA + Y +SKRT LY Y R+ G +A + GG Sbjct: 274 TPLWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPVFGGG 333 Query: 347 TVKAGSNQTGVMMGIRHTF 365 A NQT + +G+RH F Sbjct: 334 IQSATGNQTALRLGMRHRF 352 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 137 bits (344), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 126/372 (33%), Positives = 176/372 (47%), Gaps = 39/372 (10%) Query: 19 GQAHAQDAVSIYGIIDTGVLYTTNANANGNGVFKMPSLTGTFPSRLGFKDAEDLGGGNQA 78 G A AQ V+IYGI+DT + Y +NANA GN +M + SR+GFK EDLGGG +A Sbjct: 17 GAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDN-GAIANSRIGFKGTEDLGGGLKA 75 Query: 79 LFVLEAGFGPDSGVMGQGGRLFGRQSWVGLKGNWGQVSLGRQYAMTYIAMQKTDVMGPNI 138 +F LE GF D+G M QGG LFGRQSWVGL+G +G+V +GRQ + + D P Sbjct: 76 MFQLENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQNTPLFDLL--ADHFDPLT 133 Query: 139 ISISSLDAYLPNA----RTDNAIGYIGTFEGITLGATYSFGRDASAAG-----GPAATNC 189 + + +++LP A R+ N + Y G F + A+++FG A + A Sbjct: 134 VGNYASNSWLPAAASLVRSPNMLRYDGDFGPVAASASWAFGEKAGSVRTGSQFSSALRYT 193 Query: 190 AGEVA---GDAKACRQLTGLLGYERREFGINTSYDVLRGGSGAAGGLSNSDAKDQRVGLN 246 AG +A G + L + + ++ SYD G + GG N KD GL Sbjct: 194 AGPLALGGGYQQTVSSTNSSL--KHTTWNLSASYDF--GVAKVFGGWLN--IKDDS-GLT 246 Query: 247 GYFFV--GDLKFGLGLLNRRTDAVANVHTRSNLWYLGVSYPLSAALILD----------- 293 G GD+ G + V + N ++LG SY + AL L Sbjct: 247 GSVMAADGDVSNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLTGAFYYDRSKNV 306 Query: 294 AQVSRLDVKDSPNDVDLAVARLTYMLSKRTALYTSLGYLRNHGTSAIAIDSGGTVKAGSN 353 + VS +V D V Y LSKRT LYT++ Y N A + + ++ Sbjct: 307 SYVSTGNVGDGKRYT--LVGWADYALSKRTQLYTTIDY--NKAKDAAVYELTNSANGKNS 362 Query: 354 QTGVMMGIRHTF 365 T V +GIRH F Sbjct: 363 LTTVGVGIRHIF 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,155,268 Number of extensions: 51122 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 14 Length of query: 365 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 279 Effective length of database: 1,212,660 Effective search space: 338332140 Effective search space used: 338332140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)