BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0117.p (237 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 260 8e-71 gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 103 1e-23 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 260 bits (664), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 133/232 (57%), Positives = 160/232 (68%), Gaps = 7/232 (3%) Query: 4 SFIFPPQAIVGLPVDGSAVDFPVRRIYCVGRNYAAHAREMGFDPNREPPFFFCKPNDDAS 63 SF P A + V G A FPVRRIYCVGRNYAAHAREMG DP+REPPFFF KP D + Sbjct: 2 SFAIPAPAQATVAVVGGAA-FPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPAD--A 58 Query: 64 VIPVAANVTGALPYPGNTTNYHHEIELVVAIGKGGSNIAVEHAASHIFGYAVGLDMTRRD 123 V+P + V PYP T++YHHEIELVVA+ KGG NI VE A +FGYAVGLDMTRRD Sbjct: 59 VVPTDSVV----PYPVATSDYHHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRD 114 Query: 124 LQMAMRQQGRPWEIGKAFDYSAPIGPLRPIAMTGELSSGEISLQVDGKTVQSSDIANLIW 183 +Q ++ GRPW++ K FD SAP P+ P+A G G + L+V+G+ Q D+A+LIW Sbjct: 115 IQSQAKKLGRPWDMAKGFDQSAPCAPIVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIW 174 Query: 184 SVNETIANLSTLFSLEPGDLIMTGTPEGVGPVSPGQILTGHIAGLTSISVQV 235 SV ETI+ LS L L PGDLI TGTPEGVG V G L GH+ GL I++ + Sbjct: 175 SVAETISYLSGLVELFPGDLIFTGTPEGVGAVVKGDKLQGHVDGLPDINITI 226 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 103 bits (257), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 70/191 (36%), Positives = 103/191 (53%), Gaps = 15/191 (7%) Query: 26 VRRIYCVGRNYAAHAREMGFDPNREPPFFFCKPNDDASVIPVAANVTGALPYPGNTTNYH 85 + + C+G NYA HA E EP F N S + V N +P T++ Sbjct: 70 IGKFICIGLNYADHAAESNLPIPAEPVVF----NKWTSAV-VGPNDNVKIPRGSKKTDW- 123 Query: 86 HEIELVVAIGKGGSNIAVEHAASHIFGYAVGLDMTRRDLQMAMRQQGRPWEIGKAFDYSA 145 E+EL V IGKGGS I + A SH+ GY V D++ R+ Q+ ++G W+ GK D Sbjct: 124 -EVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQI---ERGGTWDKGKGCDTFG 179 Query: 146 PIGPLRPIAMTGELSSGE---ISLQVDGKTVQSSDIANLIWSVNETIANLSTLFSLEPGD 202 PIGP + E++ + + L+VDGK Q+ + + +I++V ++ LS SL+PGD Sbjct: 180 PIGPW--LVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGD 237 Query: 203 LIMTGTPEGVG 213 +I TGTP GVG Sbjct: 238 VISTGTPPGVG 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 836,136 Number of extensions: 36713 Number of successful extensions: 106 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 2 Length of query: 237 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 155 Effective length of database: 1,231,600 Effective search space: 190898000 Effective search space used: 190898000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)