BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0115.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 135 8e-33 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 86 6e-18 gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 65 1e-11 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 135 bits (339), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 94/327 (28%), Positives = 154/327 (47%), Gaps = 14/327 (4%) Query: 31 GIKITLLEQASQLGEIGAGIQLAPNAFAALDALGVGENARNKAVFTDRLIMMDAIDGKEV 90 G + + EQA +GAGI + PN L +G+ + + D G + Sbjct: 27 GFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGDVL 86 Query: 91 GTFPVDDKFRRRFDNPYAVIHRADIHTSILEEVRKNANIQFVTSSQVIDVQEDDNGVTVV 150 P+ D + + Y +HR D H +++ + + ++ + + ED V ++ Sbjct: 87 AQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPS----ERLSFGKCLTKVEDRGDVVIL 142 Query: 151 DAQDRKFN-ADALIGCDGVKSVVREKIVG-DDVRVSGHVVYRAVVPA-SLMPEDLRWNAP 207 D AD +IG DGV S +RE+++G + + +G++ +RAV P L L ++A Sbjct: 143 SFADGTTEEADIVIGADGVNSRIREELLGVEPPKYAGYLAHRAVFPTPELKAGMLPFDAC 202 Query: 208 VVWTGPHCHLVHYPLRNGEQYNLVVTFHSRDQEVWNVRD----GSKEEVLSYFTDICPQA 263 V W H++ Y + + VT E W++ D SKEE+ F P Sbjct: 203 VKWWSDDRHMMVYFVTSKANELYYVT--GVPVEKWDLNDRWLPSSKEEMRETFHGWHPTV 260 Query: 264 RQLLDTPSSWRRWSTADRNPVANWSTSRMTLLGDAAHPMLQYLAQGACMALEDAVTLGAA 323 + L+D +WS +R+P+ WS R+ LLGDA HPM ++AQGA MA+ED L Sbjct: 261 QALVDATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMKPHMAQGAAMAIEDGAMLARC 320 Query: 324 VKHCDF-DMAAAFQLYQHSRISRTARV 349 K + A AF LY+ +R +R ++V Sbjct: 321 FKEVGVSNYADAFALYEANRAARASKV 347 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 85.9 bits (211), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 92/354 (25%), Positives = 145/354 (40%), Gaps = 17/354 (4%) Query: 12 LIVGGGIGGMAAALALAKLGIKITLLEQASQLGEIGAGIQLAPNAFAALDALGVGENARN 71 LI+GGG GM+AA+ L + G ++ L+E +Q GAGI L P AL LGV E Sbjct: 9 LIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAFLR 68 Query: 72 KAVFTDRLIMMDAIDGKEVGTFPVDDKFRRRFDNPYAVIHRADIHTSILEEVRKNANIQF 131 + D + + G +V P A++ + IL + + A Sbjct: 69 EGAAGDG-VRLCLPHGPQVAELPTPRLASPDVPGGGAILR--PVLARILADATRAAGADV 125 Query: 132 VTSSQVIDVQEDDNGVTV--VDAQDRKFNADALIGCDGVKSVVREKIV--GDDVRVSGHV 187 V+E + V V D Q R++ D +IG DG+ S +R + R SG Sbjct: 126 RLGCTFSAVREVGHKVEVDFTDGQTRRY--DLVIGADGLYSKLRMHLFPHAPKPRYSGQA 183 Query: 188 VYRAVVPASLMPEDLRWNAPVVWTGPHCHLVHYPLRNGEQYNLVVTFHSRDQEVWNVRDG 247 V+RAV+P P+++ +W GP P+ E Y V ++ V Sbjct: 184 VWRAVLP---RPQEI--VTCTMWMGPRIKPGVNPVSKDEMYLFVTEPRPVNEHVDPTTFV 238 Query: 248 SKEEVL--SYFTDICPQARQLLDTPSSWRRWSTADRNPVANWSTSRMTLLGDAAHPMLQY 305 S L + + R+ LD + W R+ L+GDA H + Sbjct: 239 SHLRGLLEEFSAPVLKTIREQLDDNARIIFRPLEGLLLPRPWYQGRVVLIGDAVHATTPH 298 Query: 306 LAQGACMALEDAVTLGAAVKHCDFDMAAAFQLYQHSRISRTARVVLSAREMGRL 359 LA GAC+ +EDA+ L ++ + A ++ R R VV ++ +G + Sbjct: 299 LASGACIGIEDALVLADELER-HGTVPQALAAFEERRWERCRMVVENSARLGEI 351 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 83/348 (23%), Positives = 138/348 (39%), Gaps = 34/348 (9%) Query: 13 IVGGGIGGMAAALALAKLGIKITLLEQASQLGEIGAGIQLAPNAFAALDALGVGENARNK 72 I G G G+A AL L + G +++LLE+ Q +G+G+ L P L LG+ Sbjct: 8 IAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGLFSRIAAL 67 Query: 73 AVFTDRLIMMDAIDGKEVGTFPVDDKFRRRFDNPYAV-IHRADIHTSILEEVRKNANIQF 131 DRL DA+ GK V +D ++ Y + +HRA + +LE ++ I+ Sbjct: 68 GQRIDRLYGADALSGKTV----LDVRYDAIAGGRYGIAVHRAALFGVLLEAAQQE-GIEV 122 Query: 132 VTSSQVIDVQEDDNGVTVVDAQDRKFNA-DALIGCDGVKSVVREKIVGDDVRVSGHVVYR 190 T + +++ + A R+ D +I G +S +R + H Sbjct: 123 ETGQDIGNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHAL--------HSCEP 174 Query: 191 AVVPASLMPEDLRWNAPVVWTGPHCHLVHYPLRNGEQYNLVVTFHSRDQE------VWNV 244 +P LR + + H LV R ++ Q+ W+ Sbjct: 175 RPLPYGAFWVSLRAQPGLSFG--HNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFWST 232 Query: 245 R----DGSKEEVLSYFTD----ICPQARQLLDTPSSWRRWSTA--DRNPVANWSTSRMTL 294 R + K+ LS + D + P+ L + + + A + + R+ + Sbjct: 233 RPDRVESLKQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYGERLAI 292 Query: 295 LGDAAHPMLQYLAQGACMALEDAVTLGAAVKHCDFDMAAAFQLYQHSR 342 +GDAAH L QGA MAL DA L A++ + + A Y SR Sbjct: 293 IGDAAHSTSPQLGQGANMALLDARALSHALQQSE-TLEQALPRYAASR 339 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,210,652 Number of extensions: 49837 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 3 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)