BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0108.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310486|ref|YP_003774578.1| major facilitator superfamily p... 63 5e-11 >gi|300310486|ref|YP_003774578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 430 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 80/310 (25%), Positives = 136/310 (43%), Gaps = 38/310 (12%) Query: 2 KTIKDAASP-----WWLVGGAFLALAASTYPVIFFTFAIFLKPLAADLGWSRSVLSAALL 56 +++ +A +P W +VG FL L S I T ++ + PL + GW+RS +S + Sbjct: 6 QSLANAVTPRFHYAWVVVGVIFLVLLCSAG--IRATPSVMILPLEQEFGWNRSTISVVIS 63 Query: 57 VITVMTVVVSPLIGRLVDRIGVSYVVVPSIVVLSLAICSLGWVTDMYGYFLV---FTVIG 113 V + ++ P + R G+ VV+ + +VL + +L + M + L+ V Sbjct: 64 VNIALYGLIGPFSAAAMQRFGIRRVVL-AALVLLASGTALSTLMHMPWHMLLSWGLLVGA 122 Query: 114 ISGVAHSPLSYSREIARRFIGNRGLALGLATAGIGIGGAVMPVLAQHLIANEGWRHTYQI 173 +GVA + L + ++R F RGLA+GL TA G V L ++ + GWR + Sbjct: 123 GTGVAANTLG-ATIVSRWFETRRGLAMGLLTASAATGQMVFLPLMAAMVGSYGWRSVAFL 181 Query: 174 LGGIVLIVGLGGFLVFRRGYGVPEKQEQKGALTAGEEPRIKLFG---------------- 217 + + L+ +L+ +PE G G+ IK G Sbjct: 182 VSAVALLAIPLVWLL------LPESPAAIGQKMLGQTSEIKDEGLSRRNPLFIALDALRT 235 Query: 218 ---SPTFWLLAGAFFLMGTGVNGTI-SHLSAILTDQGAATQIAAIAQSVVGISMIAGRLM 273 P FWLL +FF+ G NG I + A+ D G +A + +G+ + G + Sbjct: 236 SVRMPDFWLLFASFFVCGLSTNGYIGTQFIAMCNDYGINEVSSASILAAMGMLDLVGTTL 295 Query: 274 CGFMFDRFTP 283 G++ DR+ P Sbjct: 296 SGWLSDRYNP 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.144 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,174,715 Number of extensions: 47211 Number of successful extensions: 308 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 303 Number of HSP's successfully gapped: 4 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)