BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0105.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 231 5e-62 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 227 1e-60 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 218 4e-58 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 84 1e-17 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 231 bits (589), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 129/323 (39%), Positives = 181/323 (56%), Gaps = 8/323 (2%) Query: 1 MSQQRTPVVVREVRQETGDIKSFFLTGVDGAVLPPFKPGDHIDIELQEGTVRQYSLCNGP 60 M+ + V R R+E DI + L VDGA LP F G HID+ L G VRQYSLCN P Sbjct: 1 MTTLQVKVAAR--REEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAP 58 Query: 61 LETDAYHISVKKEAASRGGSRILHENVKEGDVLHISPPRTNFPLTTEATRHIFIAGGIGI 120 E Y I V ++ SRGGS +HE +K GD+L IS PR F L A + +AGGIG+ Sbjct: 59 HEQHRYQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALVP-AQHSLLLAGGIGV 117 Query: 121 TPILSMLRHLQHENRPCELHYFTRTEPDTPFRGELKASLQSA--LHTGLDAERTKEQLAN 178 TPIL M L E+HY R+ FR + AS +A +H D E ++ + Sbjct: 118 TPILCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLD 177 Query: 179 ILSSRTD-GSHLFFCGPAPFMSAIRALASSLGWPEDSVHYEYFSPPDTAATATVDTPFQV 237 L ++ D +HL+ CGPA F++ + A + GWPE VH+EYF A + F+V Sbjct: 178 ALLAQADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPVKADG--NGAFEV 235 Query: 238 YLAKSQCTFDVPAGKTILEVLRAGKIPAESSCERGTCGACVVKVLEGTPEHRDSFLSDFQ 297 LA S + +P +T+++VL + SCE+G CG C+ +VLEG P+HRD +L+D + Sbjct: 236 KLASSGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEE 295 Query: 298 KKSGKKIALCVSRSQSPLLTIDL 320 + + + C SR++S LL +DL Sbjct: 296 RAANDQFTPCCSRARSALLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 227 bits (578), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 131/320 (40%), Positives = 175/320 (54%), Gaps = 9/320 (2%) Query: 10 VREVRQETGDIKSFFLTGVDGAVLPPFKPGDHIDIELQEGTVRQYSLCNGPLETDAYHIS 69 VR VRQ DI+ L DGA LPP+ PG H+ ++LQ+G VR YSLCN P + D Y I+ Sbjct: 20 VRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDCYLIA 79 Query: 70 VKKEAASRGGSRILHENVKEGDVLHISPPRTNFPLTTEATRHIFIAGGIGITPILSMLRH 129 VK+E SRGGS LHE V+EG + SPP FPL + + +A GIGITP+ +M Sbjct: 80 VKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHAMAAE 139 Query: 130 LQHENRPCELHYFTRTEPDTPFRGELKASLQS-ALHTGLDAERTKEQLANILSSRTDGSH 188 L + R LHYF R+ F EL A Q +LH GLD T+ +A L++ S Sbjct: 140 LATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHLGLDGAETEHAIAAALAAAPRSSP 199 Query: 189 LFFCGPAPFMSAIRALASSLGWPEDSVHYEYFSPPDTAATATV--------DTPFQVYLA 240 L+ CGP+PF+ A R A GW E +H+E F+ P A D+ F++ L Sbjct: 200 LYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAPDSTFELVLQ 259 Query: 241 KSQCTFDVPAGKTILEVLRAGKIPAESSCERGTCGACVVKVLEGTPEHRDSFLSDFQKKS 300 +S V G++I+ + +SC G CG+C VLEG P HRDS LS ++ S Sbjct: 260 RSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDSVLSAAERAS 319 Query: 301 GKKIALCVSRSQSPLLTIDL 320 G++I CVSR L +DL Sbjct: 320 GRRILPCVSRCAGTRLVLDL 339 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 218 bits (556), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 129/318 (40%), Positives = 181/318 (56%), Gaps = 7/318 (2%) Query: 8 VVVREVRQETGDIKSFFLTGVDGAVLPPFKPGDHIDIELQEGTVRQYSLCNGPLETD-AY 66 ++V + ETGDI+SF L DGA LP ++PG HID+ G +RQYSLC GPLE Y Sbjct: 3 LIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLC-GPLEDRCGY 61 Query: 67 HISVKKEAASRGGSRILHENVKEGDVLHISPPRTNFPLTTEATRHIFIAGGIGITPILSM 126 I+VK+E+ASRGGS LH+ VK G L + PR F L A H+ GIG+TP+L+M Sbjct: 62 TIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLAM 121 Query: 127 LRHLQHENRPCELHYFTRTEPDTPFRGELKASLQSA---LHTGLDAERTKEQLANILSSR 183 L E + LHYF R F L+ + +A H + + + L L + Sbjct: 122 AYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEAG 181 Query: 184 TDGSHLFFCGPAPFMSAIRALASSLGWPEDSVHYEYFS-PPDTAATATVDTPFQVYLAKS 242 + +H++ CGPAPFM A+ AS+ E+++H E+F P A A D F+V LA S Sbjct: 182 SKEAHVYTCGPAPFMDAVVKCASAT-RSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLASS 240 Query: 243 QCTFDVPAGKTILEVLRAGKIPAESSCERGTCGACVVKVLEGTPEHRDSFLSDFQKKSGK 302 +P G I+++L I ++SC G CG CVV +LEG P+HRD+ LS +K + Sbjct: 241 GKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND 300 Query: 303 KIALCVSRSQSPLLTIDL 320 +I CVSR++S +L +DL Sbjct: 301 QICTCVSRARSGMLVLDL 318 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 74/242 (30%), Positives = 106/242 (43%), Gaps = 23/242 (9%) Query: 2 SQQRTPVVVREVRQETGDIKSFFLTGVDGAVLPPFKPGDHIDIEL---QEGTVRQYSL-- 56 S R +V R+V E+ +I SF DG L F PG +I + + E RQYSL Sbjct: 153 SGARNFIVSRKV-AESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSA 211 Query: 57 -CNGPLETDAYHISVKKEAASRGGSRILHENVKEGDVLHISPPRTNFPLTTEATRHIFIA 115 + Y ISVK+E + SR LH+ + G V+ + PP F L I+ Sbjct: 212 LLRQAGQPPQYRISVKREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALIS 270 Query: 116 GGIGITPILSMLRHLQHENRPCELHYFTRTEPDTPFRGELK--ASLQSALHTGLDAERTK 173 GG+GITP++SML RP + R FR +L+ A+ L E+ + Sbjct: 271 GGVGITPMMSMLAAALPSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPR 330 Query: 174 EQLA----------NILSSRTDGS---HLFFCGPAPFMSAIRALASSLGWPEDSVHYEYF 220 + A ++L G+ +F GP FM AI+ + LG P YE+F Sbjct: 331 QGDAAPHATGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFF 390 Query: 221 SP 222 P Sbjct: 391 GP 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 951,686 Number of extensions: 36962 Number of successful extensions: 117 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 109 Number of HSP's successfully gapped: 4 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)