BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0100.p (824 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312823|ref|YP_003776915.1| exporter of the RND superfamily... 495 e-141 gi|300313226|ref|YP_003777318.1| protein exporter of the Resista... 472 e-134 >gi|300312823|ref|YP_003776915.1| exporter of the RND superfamily protein [Herbaspirillum seropedicae SmR1] Length = 789 Score = 495 bits (1275), Expect = e-141, Method: Compositional matrix adjust. Identities = 294/772 (38%), Positives = 436/772 (56%), Gaps = 21/772 (2%) Query: 28 RVERLLFNHRAIVLLICLLVTLVLGTQIPNLSLNANFEKMIPTSHPYVVNFLKYKSDLAG 87 R+E LF R +L + L T VL L ++A FEK +PT H Y+ F +Y+ D+ G Sbjct: 12 RLEASLFRLRIPILALLLAFTAVLSYFALQLRMDAGFEKQMPTHHEYIDTFKQYRDDVLG 71 Query: 88 LGNAVRIAVATKKGTIYSPEYLETLQKINDEVFLLPGVERTNMRSLWTPSTQWVGVTEDG 147 N + I + + G+I++ E L L ++ V LP + R ++SLWTP+T +TE+G Sbjct: 72 -ANRLNIVLRARHGSIWTKEGLTRLSEMTQAVMFLPNISRLGVQSLWTPNTFVNEITEEG 130 Query: 148 MEGGPVIPNNYNGS---PQSLTQVQLNIERSGEIGQLVALDAKSSIIYVPLLDNDSATGK 204 PVIP S P+ + Q++ G +G L++ + S++I + + D A G+ Sbjct: 131 FRADPVIPGTVIPSALTPEIIEQIRNTAINGGFVGTLISRNQDSAMIVAEINEFD-AKGE 189 Query: 205 PLDYAEFSGRLEELRAKYETSDITLHITGFAQVVGNLISGLQQMLFFFVFAILVTALMLY 264 +DY ++ L +LRAKYE +D + I GFA+ +G++ G + ++ F + A+L+TA+ +Y Sbjct: 190 KVDYLAYNKLLNDLRAKYENADFEVQIIGFAKQIGDVADGAEGVVKFCIMALLLTAIAVY 249 Query: 265 WYTRCLRSTLLVVVSSMVAVVWQLGILPLAGFQLDPYSILVPFLVFAIGMSHGAQKMNGI 324 WY R L TLL V S+V++VWQ G L L G+ LDP ++LVPFLVFAIG+SHG Q++N I Sbjct: 250 WYCRSLPFTLLPVACSLVSLVWQFGTLRLLGYGLDPLAVLVPFLVFAIGVSHGVQQINTI 309 Query: 325 LQDIGRGTHRVVAARYTFRRLFLAGLTALLCDAVGFAVLKVIEIKVIQDLAAIASIGVAV 384 ++ I G AAR +F+ L + G+ AL+ V F L +I I ++++LA ASIGV Sbjct: 310 VRGIASGQSCEAAARASFKGLLIPGVLALVTALVSFVTLVMIPIPMVRELAITASIGVGY 369 Query: 385 LVFTNLIMLPIFLSYVGVGQKAARRSIAGELEYSSPAEKHPAWRFFDLFTRR---KWASI 441 + TNL+MLP+ S + VG+ A ++ + R+ + +R + A Sbjct: 370 KIITNLVMLPLAASLLKVGKTYADAAMRAQ---------QARGRWLAVLSRSAQPRVARS 420 Query: 442 TVVAATLMAVGGFIVSLDLQVGDLDPGAPELRAESRYNKDNAFINRYYAAGSDVYAVMVK 501 VV L+ G S D VG L PGAPEL A++RYN+D +I Y G D V++ Sbjct: 421 IVVITLLVLAGSAWYSHDRLVGTLQPGAPELHADARYNRDARWIADNYGNGLDWLTVILA 480 Query: 502 TPDAECASYDTLMKVDALEWKLRQLNGVDSTVSLAGLNRRVLVGLNEGNPKWFDL---VK 558 TP C D + +R + GV S S A + G NEGNP F + Sbjct: 481 TPANSCDQVRFGRYQDQFDTVMRHVPGVLSVSSFADQMKIYNEGYNEGNPAMFTVPLDAG 540 Query: 559 SQATLNMVTSGAPRGLYNGSCNLLTVYAFLRDHKAETLTRVNDAVNEFSAANNSERAQFL 618 + A L V G RG + CN+L + FL DHKA T+ V AV + + E + Sbjct: 541 NYAAL-AVEIGRVRGFLSKDCNMLASHLFLADHKAATINGVIQAVKRYRDQHKLEGLEIR 599 Query: 619 LAAGPAGIEAATNIVVKKASHEMLLWVYGAVIILCLLTFRSWRAVVCAVLPLVLTSILCE 678 LAAG AG+ AA N V+K+ M+L+VYGA+++L +R WRA++ LPL + + + Sbjct: 600 LAAGNAGVIAAVNDEVEKSELPMMLYVYGAIVLLVFAVYRDWRAIIACCLPLTVATFIGY 659 Query: 679 ALMVKLNMGVKVATLPVIALGVGIGIDYALYVMSITLTKMREGKSLSEAYYDALTFTGKV 738 A M +L +G+ VATLPV+ L VGIG+DYA Y+ + + G ++ EA ++A+ G Sbjct: 660 AFMKQLQIGLTVATLPVMVLAVGIGVDYAFYIYNRLQLHLAAGLAIEEALHNAIQEVGIA 719 Query: 739 VMLTGVTLAAGVATWAFSPIKFQADMGILLAFMFLWNMLGALILLPALAYFL 790 + T +TLA GVATW+FS +KFQADMG LLAFMFL N++ A+ LPA A L Sbjct: 720 TIFTAITLAIGVATWSFSDLKFQADMGKLLAFMFLVNLIMAMTALPAFAVVL 771 >gi|300313226|ref|YP_003777318.1| protein exporter of the Resistance-Nodulation-Cell Division (RND) superfamily protein [Herbaspirillum seropedicae SmR1] Length = 755 Score = 472 bits (1214), Expect = e-134, Method: Compositional matrix adjust. Identities = 284/742 (38%), Positives = 417/742 (56%), Gaps = 19/742 (2%) Query: 57 NLSLNANFEKMIPTSHPYVVNFLKYKSDLAGLGNAVRIAVATKKGTIYSPEYLETLQKIN 116 L ++A FEK IP+ H YV +Y+ +L G N + I + + G+I++ E L L + Sbjct: 7 QLRMDAGFEKQIPSDHEYVATQHQYRDELFG-ANRLNIVLRARHGSIWTKEGLTRLNAMT 65 Query: 117 DEVFLLPGVERTNMRSLWTPSTQWVGVTEDGMEGGPVIPNNYNGSPQSLT-----QVQLN 171 V LP V R ++SLWTP+T +TE+G PVI +P+SLT ++ + Sbjct: 66 QAVMFLPNVNRLGVQSLWTPNTFVNEITEEGFRADPVIAGTV--TPESLTPEVIASIRDS 123 Query: 172 IERSGEIGQLVALDAKSSIIYVPLLDNDSATGKPLDYAEFSGRLEELRAKYETSDITLHI 231 G +G LV+ S++I + + D A GK +DY ++ L +LRAKYE +D + I Sbjct: 124 ANNGGFVGTLVSRKQDSAMIIAEINEFD-ANGKKVDYLAYNKLLNDLRAKYENADFEVQI 182 Query: 232 TGFAQVVGNLISGLQQMLFFFVFAILVTALMLYWYTRCLRSTLLVVVSSMVAVVWQLGIL 291 GFA+ +G++ G Q +L F + A+L+TAL +YWY R L TLL +V S+V++VWQ G L Sbjct: 183 IGFAKQIGDVADGAQGVLKFCIMALLLTALAVYWYCRSLPFTLLPIVCSLVSLVWQFGTL 242 Query: 292 PLAGFQLDPYSILVPFLVFAIGMSHGAQKMNGILQDIGRGTHRVVAARYTFRRLFLAGLT 351 L G+ LDP ++LVPFLVFAIG+SHG Q++N I++ I G AAR +FR L + G+ Sbjct: 243 HLLGYGLDPLAVLVPFLVFAIGVSHGVQQINYIVRGIAAGMGCEEAARASFRGLLVPGVL 302 Query: 352 ALLCDAVGFAVLKVIEIKVIQDLAAIASIGVAVLVFTNLIMLPIFLSYVGVGQKAARRSI 411 AL+ V F L +I I ++++LA ASIGV + TNL+MLP+ S + V + A ++ Sbjct: 303 ALVTAFVSFITLTLIPIPMVKELAITASIGVGYKIITNLVMLPLAASLLKVNKAYADAAM 362 Query: 412 AGELEYSSPAEKHPAW-RFFDLFTRRKWASITVVAATLMAVGGFIVSLDLQVGDLDPGAP 470 + +K W + F + A + VV + S D VG L GAP Sbjct: 363 RAQ-------QKRARWLQVFARAAHPRNARVIVVVTLALFAASVWYSSDRLVGTLQAGAP 415 Query: 471 ELRAESRYNKDNAFINRYYAAGSDVYAVMVKTPDAECASYDTLMKVDALEWKLRQLNGVD 530 ELRA+SRYN D I+ Y G D +V++ TP+ C + + +R + GV Sbjct: 416 ELRADSRYNLDAKSISDNYDTGLDWLSVILATPENSCDKVAFGRYQEQFDAAMRHVPGVM 475 Query: 531 STVSLAGLNRRVLVGLNEGNPKWFDLVKSQATLN--MVTSGAPRGLYNGSCNLLTVYAFL 588 S S A + G NEGNP + + A V G RG + C++L + +L Sbjct: 476 SVSSFAEQMKIYNEGYNEGNPSMYSVPLDPANYAALAVEIGRVRGFLSKDCSMLATHLYL 535 Query: 589 RDHKAETLTRVNDAVNEFSAANNSERAQFLLAAGPAGIEAATNIVVKKASHEMLLWVYGA 648 DHKA T+ + AV ++ AA+ LAAG AG+ AA N V+K+ M+L+VY A Sbjct: 536 TDHKASTINGIIAAVKQYRAAHPLPGLTIRLAAGNAGVIAAVNEEVEKSELPMMLYVYAA 595 Query: 649 VIILCLLTFRSWRAVVCAVLPLVLTSILCEALMVKLNMGVKVATLPVIALGVGIGIDYAL 708 +I+L +R RAVV LPL + + + A M +L +G+ VATLPV+ L VGIG+DYA Sbjct: 596 IIVLVFAVYRDLRAVVACCLPLTVATFIGYAFMKQLQIGLTVATLPVMVLAVGIGVDYAF 655 Query: 709 YVMSITLTKMREGKSLSEAYYDALTFTGKVVMLTGVTLAAGVATWAFSPIKFQADMGILL 768 Y+ S + G++++ A ++ G + T +TLA GVATW+FS +KFQADMG LL Sbjct: 656 YIYSRLQMHLAAGEAIAVALEKSIQEIGVATIFTAITLAIGVATWSFSQLKFQADMGKLL 715 Query: 769 AFMFLWNMLGALILLPALAYFL 790 AFMF+ N++ A+ LPA A L Sbjct: 716 AFMFIVNLIMAMTALPAFAVVL 737 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,344,557 Number of extensions: 94359 Number of successful extensions: 346 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 2 Length of query: 824 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 731 Effective length of database: 1,179,515 Effective search space: 862225465 Effective search space used: 862225465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)